Team:UNIPV-Pavia/Calendar/August/settimana1

(Difference between revisions)

Revision as of 18:55, 2 August 2010

AUGUST: WEEK 1

Miniprep and quantification at Nanodrop of:

These samples were prepared and sent (400ng) to BMR Genomics for sequencing.

The following parts were resuspended from iGEM 2010 Distribution Kit:

both in vector <partinfo>pSB1A2</partinfo>.

Transformation (1ul) of the following parts (resuspended/already miniprepped):

Transformed cells were plated on proper LB+Amp agar plates and grown ON at right temperature:

Check for plates grown ON:

week 1

week 2

@@ Line 46: / Line 46: @@
 |}
-Transformed cells were plated on proper LB+Amp agar plates:
+Transformed cells were plated on proper LB+Amp agar plates and grown ON at right temperature:
 {|border="1" align="center"
 |-
-|'''Part''' || '''Plate resistance'''
+|'''Part''' || '''Plate resistance''' || '''Temperature'''
 |-
-|pAH123 ||rowspan="2"|  Amp 50 ug/ml
+|pAH123 ||rowspan="2"|  Amp 50 ug/ml | 30°C
 |-
-|<partinfo>BBa_J72008</partinfo>
+|<partinfo>BBa_J72008</partinfo> | 30°C
 |-
-|<partinfo>BBa_R0062</partinfo> || rowspan="2"| Amp 100 ug/ml
+|<partinfo>BBa_R0062</partinfo> || rowspan="2"| Amp 100 ug/ml | 37°C
 |-
-|<partinfo>BBa_K081008</partinfo>
+|<partinfo>BBa_K081008</partinfo> | 37°C
 |-
 |}
-Plates were let grow ON at 37°C.
 ==August, 3rd==