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Team:UNIPV-Pavia/Calendar/August
From 2010.igem.org
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<partinfo>BBa_R0062</partinfo> and <partinfo>BBa_K081009</partinfo> resuspended from iGEM 2010 Distribution Kit. | <partinfo>BBa_R0062</partinfo> and <partinfo>BBa_K081009</partinfo> resuspended from iGEM 2010 Distribution Kit. | ||
| - | Trasformation of pAH123 | + | Trasformation of pAH123 and <partinfo>BBa_J72008</partinfo> helper into MC1061 and MG1655. |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/August/settimana1 #August, 3rd|August, 3rd]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/August/settimana1 #August, 3rd|August, 3rd]]</td><td align="left"> | ||
46° Bio-Lab - | 46° Bio-Lab - | ||
| + | <partinfo>BBa_R0062</partinfo> and <partinfo>BBa_K081009</partinfo> single colony pick from plates. | ||
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| + | MC1061 and MG1655 colonies transformed with helpers were picked from plates and inoculated and and incubated in proper medium. | ||
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| + | PCR from colony (MG1655 and MC1061) using synthesized primers to check them and to verify a negative control for integration. | ||
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</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/August/settimana1 #August, 4th|August, 4th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/August/settimana1 #August, 4th|August, 4th]]</td><td align="left"> | ||
47° Bio-Lab - | 47° Bio-Lab - | ||
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| + | Glycerol stocks for MC1061 and MG1655 strains transformed with pAH123 or <partinfo>BBa_J72008</partinfo> helper plasmids. Miniprep of MC1061 and MG1655 to check again the presence of helper plasmids. Digestion with SpeI and gel run. We got positive samples but a bad run. So we picked again from plates, this time single colonies. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/August/settimana1 #August, 5th|August, 5th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/August/settimana1 #August, 5th|August, 5th]]</td><td align="left"> | ||
48° Bio-Lab - | 48° Bio-Lab - | ||
| + | Miniprep of newly picked colonies MC1061 and MG1655, digestion with SpeI and gel run. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/August/settimana1 #August, 6th|August, 6th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/August/settimana1 #August, 6th|August, 6th]]</td><td align="left"> | ||
Revision as of 15:49, 5 August 2010
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