http://2010.igem.org/wiki/index.php?title=Team:UNAM-Genomics_Mexico/fr/Project&feed=atom&action=historyTeam:UNAM-Genomics Mexico/fr/Project - Revision history2024-03-29T07:56:37ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:UNAM-Genomics_Mexico/fr/Project&diff=28791&oldid=prevHmedina at 02:20, 21 July 20102010-07-21T02:20:24Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Le troisième système de réception reposera sur un système semblable à celui du EnvZ-OmpR. Ce nouveau dispositif utilise une protéine sensible à la lumière, appelée CcaS, qui possède de l'activité kinase lorsqu'elle reçoit de la lumière verte. En conséquence, un facteur de transcription appelé CcaR est phosphorilé. On constate alors la transcription des gènes en dessous du promoteur associé.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Le troisième système de réception reposera sur un système semblable à celui du EnvZ-OmpR. Ce nouveau dispositif utilise une protéine sensible à la lumière, appelée CcaS, qui possède de l'activité kinase lorsqu'elle reçoit de la lumière verte. En conséquence, un facteur de transcription appelé CcaR est phosphorilé. On constate alors la transcription des gènes en dessous du promoteur associé.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===L'Émission ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===L'Émission ===</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>En ce qui concerne les émissions bleue et verte, on considère l'opéron Lux de Vibrio fischeri la meilleur option. On veut profiter de deux protéines déjà au Registry: la lumazine pour générer une lumiére plus bleue, et LuxY pour une lumiére plus verte.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>En ce qui concerne les émissions bleue et verte, on considère l'opéron Lux de Vibrio fischeri la meilleur option. On veut profiter de deux protéines déjà au Registry: la lumazine pour générer une lumiére plus bleue, et LuxY pour une lumiére plus verte.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Les Applications ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Les Applications ===</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>On sait bien que les bactéries qui brillent dans le noir ne sont pas des nouveautés. Cependant, des bactéries qui communiquent avec de la lumière son quelque châssis d'assez rare. On conçoit diverses preuves de principe, parmi lesquelles il y a un oscillateur, un bio-câble, et une plateforme à téléphonie portable.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>On sait bien que les bactéries qui brillent dans le noir ne sont pas des nouveautés. Cependant, des bactéries qui communiquent avec de la lumière son quelque châssis d'assez rare. On conçoit diverses preuves de principe, parmi lesquelles il y a un oscillateur, un bio-câble, et une plateforme à téléphonie portable.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Les Résultats ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Les Résultats ==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(Pas encore... mais vérifiez bientôt de retour!)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(Pas encore... mais vérifiez bientôt de retour!)</div></td></tr>
</table>Hmedinahttp://2010.igem.org/wiki/index.php?title=Team:UNAM-Genomics_Mexico/fr/Project&diff=28790&oldid=prevHmedina: /* Results */2010-07-21T02:19:58Z<p><span class="autocomment">Results</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>On sait bien que les bactéries qui brillent dans le noir ne sont pas des nouveautés. Cependant, des bactéries qui communiquent avec de la lumière son quelque châssis d'assez rare. On conçoit diverses preuves de principe, parmi lesquelles il y a un oscillateur, un bio-câble, et une plateforme à téléphonie portable.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>On sait bien que les bactéries qui brillent dans le noir ne sont pas des nouveautés. Cependant, des bactéries qui communiquent avec de la lumière son quelque châssis d'assez rare. On conçoit diverses preuves de principe, parmi lesquelles il y a un oscillateur, un bio-câble, et une plateforme à téléphonie portable.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>== <del class="diffchange diffchange-inline">Results </del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>== <ins class="diffchange diffchange-inline">Les Résultats </ins>==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>(<del class="diffchange diffchange-inline">None yet</del>... <del class="diffchange diffchange-inline">but check back soon</del>!)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>(<ins class="diffchange diffchange-inline">Pas encore</ins>... <ins class="diffchange diffchange-inline">mais vérifiez bientôt de retour</ins>!)</div></td></tr>
</table>Hmedinahttp://2010.igem.org/wiki/index.php?title=Team:UNAM-Genomics_Mexico/fr/Project&diff=28789&oldid=prevHmedina: /* Application */2010-07-21T02:19:13Z<p><span class="autocomment">Application</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>En ce qui concerne les émissions bleue et verte, on considère l'opéron Lux de Vibrio fischeri la meilleur option. On veut profiter de deux protéines déjà au Registry: la lumazine pour générer une lumiére plus bleue, et LuxY pour une lumiére plus verte.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>En ce qui concerne les émissions bleue et verte, on considère l'opéron Lux de Vibrio fischeri la meilleur option. On veut profiter de deux protéines déjà au Registry: la lumazine pour générer une lumiére plus bleue, et LuxY pour une lumiére plus verte.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=== <del class="diffchange diffchange-inline">Application </del>===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=== <ins class="diffchange diffchange-inline">Les Applications </ins>===</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">While glowing bacteria are nothing new</del>, <del class="diffchange diffchange-inline">photo-communicating bacteria are something quite </del>rare. <del class="diffchange diffchange-inline">We plan on using this advantage on several applications as </del>a <del class="diffchange diffchange-inline">proof-of-principle. This may include an oscillator</del>, <del class="diffchange diffchange-inline">a </del>bio-<del class="diffchange diffchange-inline">cable</del>, <del class="diffchange diffchange-inline">cell-phone-using bacteria, among others..</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">On sait bien que les bactéries qui brillent dans le noir ne sont pas des nouveautés. Cependant</ins>, <ins class="diffchange diffchange-inline">des bactéries qui communiquent avec de la lumière son quelque châssis d'assez </ins>rare. <ins class="diffchange diffchange-inline">On conçoit diverses preuves de principe, parmi lesquelles il y </ins>a <ins class="diffchange diffchange-inline">un oscillateur</ins>, <ins class="diffchange diffchange-inline">un </ins>bio-<ins class="diffchange diffchange-inline">câble</ins>, <ins class="diffchange diffchange-inline">et une plateforme à téléphonie portable</ins>.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Results ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Results ==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(None yet... but check back soon!)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(None yet... but check back soon!)</div></td></tr>
</table>Hmedinahttp://2010.igem.org/wiki/index.php?title=Team:UNAM-Genomics_Mexico/fr/Project&diff=28786&oldid=prevHmedina: /* Emission */2010-07-21T02:13:20Z<p><span class="autocomment">Emission</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Le troisième système de réception reposera sur un système semblable à celui du EnvZ-OmpR. Ce nouveau dispositif utilise une protéine sensible à la lumière, appelée CcaS, qui possède de l'activité kinase lorsqu'elle reçoit de la lumière verte. En conséquence, un facteur de transcription appelé CcaR est phosphorilé. On constate alors la transcription des gènes en dessous du promoteur associé.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Le troisième système de réception reposera sur un système semblable à celui du EnvZ-OmpR. Ce nouveau dispositif utilise une protéine sensible à la lumière, appelée CcaS, qui possède de l'activité kinase lorsqu'elle reçoit de la lumière verte. En conséquence, un facteur de transcription appelé CcaR est phosphorilé. On constate alors la transcription des gènes en dessous du promoteur associé.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=== <del class="diffchange diffchange-inline">Emission </del>===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>===<ins class="diffchange diffchange-inline">L'Émission </ins>===</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">We plan on using a mutated </del>version <del class="diffchange diffchange-inline">of </del>Photinus pyralis <del class="diffchange diffchange-inline">luciferase to generate red light</del>. <del class="diffchange diffchange-inline">In addition</del>, <del class="diffchange diffchange-inline">we will be using a newfound </del>enzyme <del class="diffchange diffchange-inline">called </del>Luciferin Regenerating Enzyme <del class="diffchange diffchange-inline">to recycle said substrate.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">On se propose l'utilisation d'une </ins>version <ins class="diffchange diffchange-inline">mutée de l'enzyme Luciferase de </ins>Photinus pyralis <ins class="diffchange diffchange-inline">pour générer de la lumière rouge</ins>. <ins class="diffchange diffchange-inline">En plus</ins>, <ins class="diffchange diffchange-inline">on est en train d'acquérir une autre </ins>enzyme <ins class="diffchange diffchange-inline">appelée </ins>Luciferin Regenerating Enzyme <ins class="diffchange diffchange-inline">pour recycler ce substrat</ins>.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">For the blue and green emissions, we will be using the Lux Operon from Vibrio fischeri. We plan on taking advantage of the lumazine BioBrick part to generate a blue-shifted light (around 450nm), as well as the LuxY of strain Y-1 to generate a red-shifted light (around 550nm)</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">En ce qui concerne les émissions bleue et verte, on considère l'opéron Lux de Vibrio fischeri la meilleur option. On veut profiter de deux protéines déjà au Registry: la lumazine pour générer une lumiére plus bleue, et LuxY pour une lumiére plus verte.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Application ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Application ===</div></td></tr>
</table>Hmedinahttp://2010.igem.org/wiki/index.php?title=Team:UNAM-Genomics_Mexico/fr/Project&diff=28784&oldid=prevHmedina: /* Reception */2010-07-21T01:56:24Z<p><span class="autocomment">Reception</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=== <del class="diffchange diffchange-inline">Reception </del>===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=== <ins class="diffchange diffchange-inline">La Réception </ins>===</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">We plan on using a cyanobacteria phytochrome</del>-<del class="diffchange diffchange-inline">like domain coupled to an EnvZ kinase to sense incoming red light and translate it into </del>a <del class="diffchange diffchange-inline">signal detectable by the cell</del>, <del class="diffchange diffchange-inline">in this case phosphorilated </del>OmpR. <del class="diffchange diffchange-inline">This brilliant system was a construction known as </del>[http://partsregistry.org/Coliroid Coliroid] <del class="diffchange diffchange-inline">for the 2004 </del>iGEM <del class="diffchange diffchange-inline">by the University of </del>Texas <del class="diffchange diffchange-inline">at </del>Austin <del class="diffchange diffchange-inline">and </del>UCSF <del class="diffchange diffchange-inline">iGEM team</del>. <del class="diffchange diffchange-inline">This system acts as an </del><IF ! <del class="diffchange diffchange-inline">LIGHT</del>> <del class="diffchange diffchange-inline">logic gate. We are contemplating the use of distinct assemblies to obtain a direct </del><IF LIGHT> <del class="diffchange diffchange-inline">logic gate</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">On se propose l'utilisation des domaines semblables à ceux des phytochromes chez les plantes, mais cette fois</ins>-<ins class="diffchange diffchange-inline">ci chez les cyanobacteries. Ce cyanobacteriochrome sera couplé </ins>a <ins class="diffchange diffchange-inline">une domaine kinase de EnvZ pour détecter de la lumière rouge</ins>, <ins class="diffchange diffchange-inline">et alors déclencher une réponse chimique qui puisse être reconnue par la cellule, c'est-à-dire du </ins>OmpR <ins class="diffchange diffchange-inline">phosphorilé</ins>. <ins class="diffchange diffchange-inline">Ce système à été conçu comme </ins>[http://partsregistry.org/Coliroid Coliroid] <ins class="diffchange diffchange-inline">par l'équipe </ins>iGEM <ins class="diffchange diffchange-inline">2004 de l'Université de </ins>Texas <ins class="diffchange diffchange-inline">en </ins>Austin<ins class="diffchange diffchange-inline">, et l'</ins>UCSF. <ins class="diffchange diffchange-inline">Cependant, ce système possède la fonction d'une porte logique </ins><IF ! <ins class="diffchange diffchange-inline">LIGTH</ins>><ins class="diffchange diffchange-inline">, tandis que nous voulons une porte du type </ins><IF LIGHT><ins class="diffchange diffchange-inline">. On travail encore en ce petit problème..</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Our second sensing device is based on another </del>BioBrick <del class="diffchange diffchange-inline">Part</del>: [http://partsregistry.org/Part:BBa_K191003 LOVtap]. <del class="diffchange diffchange-inline">This TF dimerizes when struck by blue light, thus activating the </del>transcription <del class="diffchange diffchange-inline">of a determined promoter</del>'<del class="diffchange diffchange-inline">s coding sequence</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Notre deuxième système capteur sera fondé sur une autre parte </ins>BioBrick: [http://partsregistry.org/Part:BBa_K191003 LOVtap]. <ins class="diffchange diffchange-inline">Ce facteur de </ins>transcription <ins class="diffchange diffchange-inline">dimerize lorsqu</ins>'<ins class="diffchange diffchange-inline">il reçoit de la lumière bleue</ins>. <ins class="diffchange diffchange-inline">Il démarre donc la </ins>transcription <ins class="diffchange diffchange-inline">des gènes contenus après un certain promoteur</ins>.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Our final input device is based on a newfound OmpR-like system of Synechocystis. This system uses a sensing protein (CcaS) than shows kinase activity when struck by green light. It then proceeds to phosphorilate a regulatroy TF (CcaR). This TF then starts </del>transcription <del class="diffchange diffchange-inline">of its associated promoter's coding sequence</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Le troisième système de réception reposera sur un système semblable à celui du EnvZ-OmpR. Ce nouveau dispositif utilise une protéine sensible à la lumière, appelée CcaS, qui possède de l'activité kinase lorsqu'elle reçoit de la lumière verte. En conséquence, un facteur de transcription appelé CcaR est phosphorilé. On constate alors la transcription des gènes en dessous du promoteur associé.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Emission ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Emission ===</div></td></tr>
</table>Hmedinahttp://2010.igem.org/wiki/index.php?title=Team:UNAM-Genomics_Mexico/fr/Project&diff=28781&oldid=prevHmedina at 01:25, 21 July 20102010-07-21T01:25:59Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>On définit le phénomène de communication comme le transfert d'information par un moyen spécifique d'une entité émettrice à une entité réceptrice. Les entités biologiques dépendent des temps immémoriaux en messagers chimiques pour transmettre leurs informations. Cela est vrai tant pour des populations d'organismes unicellulaire, que pour des organismes multicellulaires. Parce que ce sont des messagers chimiques, ils sont alors contraints à l'intérieur d'un système chimique. C'est la raison pour laquelle même des messagers de portée longue comme des hormones ne dépassent pas le système chimique qu'est le corps humain.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>On définit le phénomène de communication comme le transfert d'information par un moyen spécifique d'une entité émettrice à une entité réceptrice. Les entités biologiques dépendent des temps immémoriaux en messagers chimiques pour transmettre leurs informations. Cela est vrai tant pour des populations d'organismes unicellulaire, que pour des organismes multicellulaires. Parce que ce sont des messagers chimiques, ils sont alors contraints à l'intérieur d'un système chimique. C'est la raison pour laquelle même des messagers de portée longue comme des hormones ne dépassent pas le système chimique qu'est le corps humain.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Notre objectif <del class="diffchange diffchange-inline">en </del>ce projet <del class="diffchange diffchange-inline">es</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Notre objectif <ins class="diffchange diffchange-inline">dans </ins>ce projet <ins class="diffchange diffchange-inline">s'agit de rendre la barrière chimique obsolète avec l'implémentation d'un système indépendant du strate chimique. Cela nous a conduit à l'utilisation d'un messager non-chimique, c'est-à-dire des photons. Nos moyens de communication reposent donc en lumière; des paquets des photons, ou des quanta énergétiques, vont transporter l'information à communiquer des entités réceptrices aux entités émettrices. Cela nous permet effectivement dépasser diverses barrières isolant nos entités. En conséquence, notre système de communication n'est plus contenu dans un système chimique, mais alors dans un système physique; c'est-à-dire qu'il faut qu'il reste un moyen physique pour que les photons soient transportés. Ce système physique peut donc être quelque chose de si complexe comme un dispositif de relai électronique à microcontrôleurs, comme quelque chose de si simple comme du vide. Or, ce strate physique est remarquablement étanche à la signalisation chimique. Donc, le bruit du fond chimique de notre système ne devra pas perturber significativement le strate des photons. En conséquence, on peut affirmer que l'échange d'information par moyens photoniques sera suffisamment indépendante de l'information endogène codée aux voies chimiques pour que le système marche correctement. Autrement dit, la voie photonique est extrêmement non-envahissante. En plus, nos entités réceptrices sont facilement transformées en entités émettrices. Donc, en utilisant nos cellules comme des châssis processeurs d'information, on arrive à augmenter les possibilités de communication énormément. On peut effectivement rendre nos constructions un système où l'information est:</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* d'abord, codée et émise par un émetteur,</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* puis, reçue et décodée par un récepteur,</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* et finalement, traitée, transformée, et relayé de suite.</ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">In this project</del>, <del class="diffchange diffchange-inline">our goal is to render the chemical barrier deprecated by enabling chemical-free communication. This has been translated to the implementation of a non-chemical messenger, in this case, photons. Our channel is thus light based; packages of photons, or energy quanta, will transport information from senders to receivers, effectively bypassing most chemical barriers in-between. Consequently, our communicating system is no longer contained within a chemical system, but within a physical one, ie: there must remain a physical channel where photons can be transported. This physical channel may range from something as sophisticated as a microcontroler-based electronic relay system, to something as simple as vacuum (or void). However, this physical layer proves very well to be impervious to most chemical signaling. Ergo, the chemical system's signaling would remain unaffected by the physical channel, and vice versa. In consequence, the exchange of information through physical means is sufficiently independent from the information encoded in the system's endogenous chemical pathways. In other words, it is extraordinarily uninvasive. As an added bonus, our receiver entities are easily transformed into emitter entities. Thus, by using our cells as information processing chassis, we can expand the communications layer. We can effectively render our system one where information is:</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Notre ambitieuse implémentation repose sur des composants bien connus</ins>, <ins class="diffchange diffchange-inline">principalement des ensembles bioluminescents de </ins>Photinus pyralis <ins class="diffchange diffchange-inline">et </ins>Vibrio fischeri, <ins class="diffchange diffchange-inline">des récepteurs photoactives comme les </ins>cyanobacteriochromes <ins class="diffchange diffchange-inline">des Cyanobactéries, et des domaines </ins>Light-Oxygen-Voltage <ins class="diffchange diffchange-inline">des certaines protéines chimériques</ins>. <ins class="diffchange diffchange-inline">On veut donc approfondir sur le fait que certaines organismes, des unicellulaires tant que des multicellulaires, possèdent déjà des formes de </ins>photo-communication <ins class="diffchange diffchange-inline">assez primitives</ins>. <ins class="diffchange diffchange-inline">En plus</ins>, <ins class="diffchange diffchange-inline">avec nos constructions l'</ins>information <ins class="diffchange diffchange-inline">sera changée de strate (chimique à photonique et vice versa) à l'intérieur des cellules</ins>. <ins class="diffchange diffchange-inline">En conséquence</ins>, <ins class="diffchange diffchange-inline">les membranes qu'une fois étaient des barrières à la </ins>communication<ins class="diffchange diffchange-inline">, seront transformées en isolants de bruit de fond</ins>. <ins class="diffchange diffchange-inline">Avec le découplage du messager et du strate chimique</ins>, <ins class="diffchange diffchange-inline">on rend possible tout un univers d'usages auparavant impossibles. On imagine des </ins>applications <ins class="diffchange diffchange-inline">en neurobiologie</ins>, <ins class="diffchange diffchange-inline">en couplage cybernétique</ins>, <ins class="diffchange diffchange-inline">et même en télécommunications biologiques</ins>.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*Encoded and sent by an emitter</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*Recieved and decoded by a receiver</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*Plus processed, transformed, and relayed forth</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Our ambicious implementation is based on well known systems, mainly bioluminescent proteins from </del>Photinus pyralis <del class="diffchange diffchange-inline">and </del>Vibrio fischeri, <del class="diffchange diffchange-inline">as well as photoactive receptors like Cyanobacteria </del>cyanobacteriochromes <del class="diffchange diffchange-inline">and </del>Light-Oxygen-Voltage <del class="diffchange diffchange-inline">domain quimeric proteins</del>. <del class="diffchange diffchange-inline">We thus exploit the fact that cells already display primitive </del>photo-communication<del class="diffchange diffchange-inline">, both within multicellular organisms as well as within populations of unicellular ones</del>. <del class="diffchange diffchange-inline">Moreover</del>, <del class="diffchange diffchange-inline">in our system the photonic </del>information <del class="diffchange diffchange-inline">is transformed to and from chemical information within the chemical system that is an individual cell</del>. <del class="diffchange diffchange-inline">Thus</del>, <del class="diffchange diffchange-inline">the chemical barrier that is the membrane has ceased being a barrier to </del>communication <del class="diffchange diffchange-inline">and is now a noise isolator</del>. <del class="diffchange diffchange-inline">By decoupling the messenger from the chemical layer</del>, <del class="diffchange diffchange-inline">we enable a brand new host of </del>applications <del class="diffchange diffchange-inline">that were previously unavailable, ranging in domains from neurobiology</del>, <del class="diffchange diffchange-inline">to cybernetic coupling</del>, <del class="diffchange diffchange-inline">and even to biological telecommunications</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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</table>Hmedinahttp://2010.igem.org/wiki/index.php?title=Team:UNAM-Genomics_Mexico/fr/Project&diff=28752&oldid=prevHmedina: /* Les Détails du Projet */2010-07-21T00:25:52Z<p><span class="autocomment">Les Détails du Projet</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Les Détails du Projet==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Les Détails du Projet==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>On définit le phénomène de communication comme le transfert d'information par un moyen spécifique d'une entité émettrice à une entité réceptrice. Les entités biologiques</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>On définit le phénomène de communication comme le transfert d'information par un moyen spécifique d'une entité émettrice à une entité réceptrice. Les entités biologiques <ins class="diffchange diffchange-inline">dépendent des temps immémoriaux en messagers chimiques pour transmettre leurs informations. Cela est vrai tant pour des populations d'organismes unicellulaire, que pour des organismes multicellulaires. Parce que ce sont des messagers chimiques, ils sont alors contraints à l'intérieur d'un système chimique. C'est la raison pour laquelle même des messagers de portée longue comme des hormones ne dépassent pas le système chimique qu'est le corps humain.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">The process of transferring information from a sender entity to a receiver one through a determined channel is called communication. Biological entities have relied since time immemorial on chemical messengers to relay information; this holds true for multicellular organisms as well as for populations of unicellular organisms. Being chemical based, these messengers are constrained to a chemical system regardless of the scope of said system, eg: even far reaching messengers such as hormones are bound within the chemical system that is the human body.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Notre objectif en ce projet es</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In this project, our goal is to render the chemical barrier deprecated by enabling chemical-free communication. This has been translated to the implementation of a non-chemical messenger, in this case, photons. Our channel is thus light based; packages of photons, or energy quanta, will transport information from senders to receivers, effectively bypassing most chemical barriers in-between. Consequently, our communicating system is no longer contained within a chemical system, but within a physical one, ie: there must remain a physical channel where photons can be transported. This physical channel may range from something as sophisticated as a microcontroler-based electronic relay system, to something as simple as vacuum (or void). However, this physical layer proves very well to be impervious to most chemical signaling. Ergo, the chemical system's signaling would remain unaffected by the physical channel, and vice versa. In consequence, the exchange of information through physical means is sufficiently independent from the information encoded in the system's endogenous chemical pathways. In other words, it is extraordinarily uninvasive. As an added bonus, our receiver entities are easily transformed into emitter entities. Thus, by using our cells as information processing chassis, we can expand the communications layer. We can effectively render our system one where information is:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In this project, our goal is to render the chemical barrier deprecated by enabling chemical-free communication. This has been translated to the implementation of a non-chemical messenger, in this case, photons. Our channel is thus light based; packages of photons, or energy quanta, will transport information from senders to receivers, effectively bypassing most chemical barriers in-between. Consequently, our communicating system is no longer contained within a chemical system, but within a physical one, ie: there must remain a physical channel where photons can be transported. This physical channel may range from something as sophisticated as a microcontroler-based electronic relay system, to something as simple as vacuum (or void). However, this physical layer proves very well to be impervious to most chemical signaling. Ergo, the chemical system's signaling would remain unaffected by the physical channel, and vice versa. In consequence, the exchange of information through physical means is sufficiently independent from the information encoded in the system's endogenous chemical pathways. In other words, it is extraordinarily uninvasive. As an added bonus, our receiver entities are easily transformed into emitter entities. Thus, by using our cells as information processing chassis, we can expand the communications layer. We can effectively render our system one where information is:</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>While glowing bacteria are nothing new, photo-communicating bacteria are something quite rare. We plan on using this advantage on several applications as a proof-of-principle. This may include an oscillator, a bio-cable, cell-phone-using bacteria, among others...</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>While glowing bacteria are nothing new, photo-communicating bacteria are something quite rare. We plan on using this advantage on several applications as a proof-of-principle. This may include an oscillator, a bio-cable, cell-phone-using bacteria, among others...</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Results ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Results ==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(None yet... but check back soon!)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(None yet... but check back soon!)</div></td></tr>
</table>Hmedinahttp://2010.igem.org/wiki/index.php?title=Team:UNAM-Genomics_Mexico/fr/Project&diff=28706&oldid=prevHmedina at 23:54, 20 July 20102010-07-20T23:54:26Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* D'autre part, une enquête rapide du [http://tess2.uspto.gov/ Système Électronique des Marques de Commerce], du Bureau des Patentes et des Marques de Commerce des États Unis a révèle que le mot WiFi était une marque de commerce en 2006, mais elle est listée comme "Morte" actuellement. C'est pour cela qu'on n'enfreint personne en utilisant WiFi.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* D'autre part, une enquête rapide du [http://tess2.uspto.gov/ Système Électronique des Marques de Commerce], du Bureau des Patentes et des Marques de Commerce des États Unis a révèle que le mot WiFi était une marque de commerce en 2006, mais elle est listée comme "Morte" actuellement. C'est pour cela qu'on n'enfreint personne en utilisant WiFi.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>== <del class="diffchange diffchange-inline">Project Details</del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>== <ins class="diffchange diffchange-inline">Les Détails du Projet</ins>==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">On définit le phénomène de communication comme le transfert d'information par un moyen spécifique d'une entité émettrice à une entité réceptrice. Les entités biologiques</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The process of transferring information from a sender entity to a receiver one through a determined channel is called communication. Biological entities have relied since time immemorial on chemical messengers to relay information; this holds true for multicellular organisms as well as for populations of unicellular organisms. Being chemical based, these messengers are constrained to a chemical system regardless of the scope of said system, eg: even far reaching messengers such as hormones are bound within the chemical system that is the human body.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The process of transferring information from a sender entity to a receiver one through a determined channel is called communication. Biological entities have relied since time immemorial on chemical messengers to relay information; this holds true for multicellular organisms as well as for populations of unicellular organisms. Being chemical based, these messengers are constrained to a chemical system regardless of the scope of said system, eg: even far reaching messengers such as hormones are bound within the chemical system that is the human body.</div></td></tr>
</table>Hmedinahttp://2010.igem.org/wiki/index.php?title=Team:UNAM-Genomics_Mexico/fr/Project&diff=28691&oldid=prevHmedina: /* Overall project */2010-07-20T23:43:46Z<p><span class="autocomment">Overall project</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>== '''<del class="diffchange diffchange-inline">Overall project</del>''' ==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>== '''<ins class="diffchange diffchange-inline">La Vision Générale</ins>''' ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==='''<del class="diffchange diffchange-inline">The Idea</del>'''===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==='''<ins class="diffchange diffchange-inline">L'Idée</ins>'''===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Synthetic Biology has been enabling changes in all bio-domains</del>, <del class="diffchange diffchange-inline">one such being</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">La Biologie Synthétique ouvre des nouvelles possibilités dans les domaines biologiques</ins>, <ins class="diffchange diffchange-inline">telle la </ins>communication <ins class="diffchange diffchange-inline">cellulaire</ins>. <ins class="diffchange diffchange-inline">Dés son commencement et jusqu'à nous jours, celle-ci reposait sur le principe de la </ins>communication <ins class="diffchange diffchange-inline">avec des messagers exclusivement chimiques</ins>. <ins class="diffchange diffchange-inline">Ces messagers chimiques ont alors une portée limitée: même des messagers de portée assez longue comme les </ins>hormones<ins class="diffchange diffchange-inline">, sont limités dans le système chimique qu'est le corps humain</ins>. <ins class="diffchange diffchange-inline">Mais ce paradigme sera bientôt renversé</ins>.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>communication. <del class="diffchange diffchange-inline">Cellular </del>communication <del class="diffchange diffchange-inline">has relied since time immemorial on</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">chemical messengers to exchange information</del>. <del class="diffchange diffchange-inline">As such, these messengers regardless</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">of their scope, are constrained to a chemical system; even far reaching</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">messengers such as </del>hormones <del class="diffchange diffchange-inline">are bound within the chemical system that is</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">the human body</del>. <del class="diffchange diffchange-inline">But this mode is about to change</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">In this project, our goal is to render the chemical barrier deprecated by</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Notre objectif dans ce projet s'agit de rendre la barrière chimique obsolète avec l'utilisation d'un messager </ins>non-<ins class="diffchange diffchange-inline">chimique, c'est-à-dire des </ins>photons. <ins class="diffchange diffchange-inline">Ceux-ci vont transporter l'</ins>information <ins class="diffchange diffchange-inline">à communiquer entre des cellules spécialement modifiées équipées de deux habilitées: la perception et l'émission de la lumière. On va donc créer un nouvel système de photo</ins>-<ins class="diffchange diffchange-inline">communication </ins>inter-<ins class="diffchange diffchange-inline">cellulaire</ins>.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">using a </del>non-<del class="diffchange diffchange-inline">chemical messenger: </del>photons. <del class="diffchange diffchange-inline">These will transport</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>information <del class="diffchange diffchange-inline">between cells that have been designed to sense and emit light,</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">thus creating a photon</del>-<del class="diffchange diffchange-inline">based </del>inter-<del class="diffchange diffchange-inline">cellular communication system</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">These messengers are produced through bio-luminescent reactions</del>, <del class="diffchange diffchange-inline">and</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Nos messagers seront crées par des réactions de Bioluminescence</ins>, <ins class="diffchange diffchange-inline">et ils seront assez capables de traverses des divers milieux</ins>. <ins class="diffchange diffchange-inline">Logiquement</ins>, <ins class="diffchange diffchange-inline">cela permet la </ins>propagation <ins class="diffchange diffchange-inline">de l'</ins>information <ins class="diffchange diffchange-inline">au-delà des </ins>restrictions <ins class="diffchange diffchange-inline">chimiques, biologiques, et même spatiales</ins>. <ins class="diffchange diffchange-inline">Du fait d'avoir un messager découplé du strate chimique</ins>, <ins class="diffchange diffchange-inline">notre projet rend la communication assez facile entre des systèmes organiques, et des systèmes au silicium</ins>, <ins class="diffchange diffchange-inline">tels les ordinateurs</ins>.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">are quite capable of traversing multiple environments</del>. <del class="diffchange diffchange-inline">Consecuently</del>, <del class="diffchange diffchange-inline">this enables</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">the </del>propagation <del class="diffchange diffchange-inline">of </del>information <del class="diffchange diffchange-inline">beyond the chemical, biological and even spatial</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>restrictions. <del class="diffchange diffchange-inline">As the messenger is effectively decoupled from the chemical layer</del>,</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">it is a natural step in the communications bridge between organic-based and</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">silicon-based systems</del>, <del class="diffchange diffchange-inline">such as computers</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==='''<del class="diffchange diffchange-inline">The Name</del>'''===</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==='''<ins class="diffchange diffchange-inline">Le Nom</ins>'''===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">As you might imagine, WiFi is a play on the popular IEEE 802</del>.<del class="diffchange diffchange-inline">11 communications standard knows as </del>Wi-Fi. <del class="diffchange diffchange-inline">Since our systems achieves the transfer of information without wires, it is thus wireless</del>. <del class="diffchange diffchange-inline">As for fidelity</del>, <del class="diffchange diffchange-inline">we shall see.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Vous avez sans doute reconnu le jeux de mots à l'origine du nom du projet</ins>. <ins class="diffchange diffchange-inline">Le </ins>Wi-Fi <ins class="diffchange diffchange-inline">est un standard de communication basé sur IEEE 802</ins>.<ins class="diffchange diffchange-inline">11 très connu au monde</ins>. <ins class="diffchange diffchange-inline">Non-officiellement</ins>, on <ins class="diffchange diffchange-inline">pense </ins>Wi-Fi <ins class="diffchange diffchange-inline">est né du Wireless Fidelity. Donc, parce que notre système permet la communication sans l</ins>'<ins class="diffchange diffchange-inline">utilisation des fils, il est alors wireless</ins>. <ins class="diffchange diffchange-inline">En ce qui concerne la fiabilité</ins>, on <ins class="diffchange diffchange-inline">verra</ins>...</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Before you begin wondering </del>on <del class="diffchange diffchange-inline">Copyright issues, let us make two things clear:</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">* Our name is WiFi, not the [http://www.wi-fi.org/ </del>Wi-Fi <del class="diffchange diffchange-inline">Alliance]</del>'<del class="diffchange diffchange-inline">s Wi-Fi (notice the hyphen)</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">* Second</del>, <del class="diffchange diffchange-inline">a quick search </del>on <del class="diffchange diffchange-inline">the US Patents & Trademarks Office [http://tess2</del>.<del class="diffchange diffchange-inline">uspto</del>.<del class="diffchange diffchange-inline">gov/ Trademark Electronic Search System (TESS)] returned that WiFi was a trademark in 2006, but is now listed as "Dead". Therefore, we are not infringing copyright issues by using it in our system</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Or, avant de faire l'incursion au domaine du copyright, il faut clarifier deux aspects:</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Notre nom es WiFi, et non pas le Wi-Fi du [http://www.wi-fi.org/ Wi-Fi Alliance] (notez le trait d'union).</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* D'autre part, une enquête rapide du [http://tess2.uspto.gov/ Système Électronique des Marques de Commerce], du Bureau des Patentes et des Marques de Commerce des États Unis a révèle que le mot WiFi était une marque de commerce en 2006, mais elle est listée comme "Morte" actuellement. C'est pour cela qu'on n'enfreint personne en utilisant WiFi.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Project Details==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Project Details==</div></td></tr>
</table>Hmedinahttp://2010.igem.org/wiki/index.php?title=Team:UNAM-Genomics_Mexico/fr/Project&diff=28624&oldid=prevHmedina: New page: <div id="header">{{Template:Team:UNAM-Genomics_Mexico/Templates/fr/header}}</div> == '''Overall project''' == ==='''The Idea'''=== Synthetic Biology has been enabling changes in all bi...2010-07-20T22:50:33Z<p>New page: <div id="header">{{Template:Team:UNAM-Genomics_Mexico/Templates/fr/header}}</div> == '''Overall project''' == ==='''The Idea'''=== Synthetic Biology has been enabling changes in all bi...</p>
<p><b>New page</b></p><div><div id="header">{{Template:Team:UNAM-Genomics_Mexico/Templates/fr/header}}</div><br />
<br />
== '''Overall project''' ==<br />
<br />
<br />
==='''The Idea'''===<br />
<br />
Synthetic Biology has been enabling changes in all bio-domains, one such being<br />
communication. Cellular communication has relied since time immemorial on<br />
chemical messengers to exchange information. As such, these messengers regardless<br />
of their scope, are constrained to a chemical system; even far reaching<br />
messengers such as hormones are bound within the chemical system that is<br />
the human body. But this mode is about to change.<br />
<br />
In this project, our goal is to render the chemical barrier deprecated by<br />
using a non-chemical messenger: photons. These will transport<br />
information between cells that have been designed to sense and emit light,<br />
thus creating a photon-based inter-cellular communication system.<br />
<br />
These messengers are produced through bio-luminescent reactions, and<br />
are quite capable of traversing multiple environments. Consecuently, this enables<br />
the propagation of information beyond the chemical, biological and even spatial<br />
restrictions. As the messenger is effectively decoupled from the chemical layer,<br />
it is a natural step in the communications bridge between organic-based and<br />
silicon-based systems, such as computers.<br />
<br />
<br />
==='''The Name'''===<br />
<br />
As you might imagine, WiFi is a play on the popular IEEE 802.11 communications standard knows as Wi-Fi. Since our systems achieves the transfer of information without wires, it is thus wireless. As for fidelity, we shall see.<br />
<br />
Before you begin wondering on Copyright issues, let us make two things clear:<br />
* Our name is WiFi, not the [http://www.wi-fi.org/ Wi-Fi Alliance]'s Wi-Fi (notice the hyphen).<br />
* Second, a quick search on the US Patents & Trademarks Office [http://tess2.uspto.gov/ Trademark Electronic Search System (TESS)] returned that WiFi was a trademark in 2006, but is now listed as "Dead". Therefore, we are not infringing copyright issues by using it in our system.<br />
<br />
<br />
== Project Details==<br />
<br />
The process of transferring information from a sender entity to a receiver one through a determined channel is called communication. Biological entities have relied since time immemorial on chemical messengers to relay information; this holds true for multicellular organisms as well as for populations of unicellular organisms. Being chemical based, these messengers are constrained to a chemical system regardless of the scope of said system, eg: even far reaching messengers such as hormones are bound within the chemical system that is the human body.<br />
<br />
In this project, our goal is to render the chemical barrier deprecated by enabling chemical-free communication. This has been translated to the implementation of a non-chemical messenger, in this case, photons. Our channel is thus light based; packages of photons, or energy quanta, will transport information from senders to receivers, effectively bypassing most chemical barriers in-between. Consequently, our communicating system is no longer contained within a chemical system, but within a physical one, ie: there must remain a physical channel where photons can be transported. This physical channel may range from something as sophisticated as a microcontroler-based electronic relay system, to something as simple as vacuum (or void). However, this physical layer proves very well to be impervious to most chemical signaling. Ergo, the chemical system's signaling would remain unaffected by the physical channel, and vice versa. In consequence, the exchange of information through physical means is sufficiently independent from the information encoded in the system's endogenous chemical pathways. In other words, it is extraordinarily uninvasive. As an added bonus, our receiver entities are easily transformed into emitter entities. Thus, by using our cells as information processing chassis, we can expand the communications layer. We can effectively render our system one where information is:<br />
*Encoded and sent by an emitter<br />
*Recieved and decoded by a receiver<br />
*Plus processed, transformed, and relayed forth<br />
<br />
Our ambicious implementation is based on well known systems, mainly bioluminescent proteins from Photinus pyralis and Vibrio fischeri, as well as photoactive receptors like Cyanobacteria cyanobacteriochromes and Light-Oxygen-Voltage domain quimeric proteins. We thus exploit the fact that cells already display primitive photo-communication, both within multicellular organisms as well as within populations of unicellular ones. Moreover, in our system the photonic information is transformed to and from chemical information within the chemical system that is an individual cell. Thus, the chemical barrier that is the membrane has ceased being a barrier to communication and is now a noise isolator. By decoupling the messenger from the chemical layer, we enable a brand new host of applications that were previously unavailable, ranging in domains from neurobiology, to cybernetic coupling, and even to biological telecommunications.<br />
<br />
<br />
=== Reception ===<br />
<br />
We plan on using a cyanobacteria phytochrome-like domain coupled to an EnvZ kinase to sense incoming red light and translate it into a signal detectable by the cell, in this case phosphorilated OmpR. This brilliant system was a construction known as [http://partsregistry.org/Coliroid Coliroid] for the 2004 iGEM by the University of Texas at Austin and UCSF iGEM team. This system acts as an <IF ! LIGHT> logic gate. We are contemplating the use of distinct assemblies to obtain a direct <IF LIGHT> logic gate.<br />
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Our second sensing device is based on another BioBrick Part: [http://partsregistry.org/Part:BBa_K191003 LOVtap]. This TF dimerizes when struck by blue light, thus activating the transcription of a determined promoter's coding sequence.<br />
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Our final input device is based on a newfound OmpR-like system of Synechocystis. This system uses a sensing protein (CcaS) than shows kinase activity when struck by green light. It then proceeds to phosphorilate a regulatroy TF (CcaR). This TF then starts transcription of its associated promoter's coding sequence.<br />
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=== Emission ===<br />
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We plan on using a mutated version of Photinus pyralis luciferase to generate red light. In addition, we will be using a newfound enzyme called Luciferin Regenerating Enzyme to recycle said substrate.<br />
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For the blue and green emissions, we will be using the Lux Operon from Vibrio fischeri. We plan on taking advantage of the lumazine BioBrick part to generate a blue-shifted light (around 450nm), as well as the LuxY of strain Y-1 to generate a red-shifted light (around 550nm).<br />
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=== Application ===<br />
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While glowing bacteria are nothing new, photo-communicating bacteria are something quite rare. We plan on using this advantage on several applications as a proof-of-principle. This may include an oscillator, a bio-cable, cell-phone-using bacteria, among others...<br />
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== Results ==<br />
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(None yet... but check back soon!)</div>Hmedina