Team:UNAM-Genomics Mexico/Safety

From 2010.igem.org

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<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
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=='''Safety'''==
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==='''Identifying Safety Issues'''===
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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This is a template page. READ THESE INSTRUCTIONS.
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="http://2008.igem.org/Help:Template/Examples">HERE</a>.
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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===='''Probability'''====
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''Could there be an unplanned event or series of events involving your project, resulting in either death, injury, occupational illness, damage to equipment or property, or damage to the environment? How likely is that going to happen?''
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There could always be some sinister series of unfortunate events that leads to disaster, however given the nature of our project, we believe that that little beyond minimal environmental exposure is the scope of catastrophe. Our BioBricks parts are mainly enzymes. These enzymes catalyze reactions that are already found in nature in a wide variety of environmental niches. As such, they are nothing new. Nature has found ways of balancing their actions, so even if they were to be exposed dramatically, the perturbation should be minimal and easily self-correcting.
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{|align="justify"
 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
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|[[Image:UNAM-Genomics_Mexico_logo.png|200px|right|frame]]
 
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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|[[Image:UNAM-Genomics_Mexico_team.png|right|frame|Your team picture]]
 
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|align="center"|[[Team:UNAM-Genomics_Mexico | Team Example]]
 
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''Does your project require the exposure or release of the engineered organism to people or the environment (e.g. as medicine, for bioremediation)?''
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Our project does not require exposure or release to either people or environment. In fact, this project is meant to be tested under extreme biological, chemical and even thermal isolation. One of the points we want to prove is the fact that we can communicate information where there is no possible biological, chemical or even thermal bridge spanning our communicating entities. They will be isolated, both from each other as from the environment.
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!align="center"|[[Team:UNAM-Genomics_Mexico|Home]]
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!align="center"|[[Team:UNAM-Genomics_Mexico/Team|Team]]
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!align="center"|[http://igem.org/Team.cgi?year=2010&team_name=UNAM-Genomics_Mexico Official Team Profile]
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!align="center"|[[Team:UNAM-Genomics_Mexico/Project|Project]]
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!align="center"|[[Team:UNAM-Genomics_Mexico/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:UNAM-Genomics_Mexico/Modeling|Modeling]]
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!align="center"|[[Team:UNAM-Genomics_Mexico/Notebook|Notebook]]
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!align="center"|[[Team:UNAM-Genomics_Mexico/Safety|Safety]]
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===='''Hazard'''====
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==Safety==
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''Could your device, when working properly, represent a hazard to people or the environment?''
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Please use this page to answer the safety questions posed on the [[Safety | safety page]].
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We do not believe our device represents a hazard. If working properly, our devices will stress the host organism without generating any short-term benefit. As such, they are only an added metabolic process to the host. At most, our devices would generate a higher-than-average amount of reactive oxygen species, but not in a significant manner. Perhaps, should the reactions be extreme in number, the light emission could be a hazard to the naked eye...
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''Is your engineered organism infectious? Does it produce a toxic product? Does it interfere with human physiology or the environment?''
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Our engineered organism is not infectious, nor does it produce toxic products. The only dangerous product of our devices are reactive oxygen species, however their net production is not significant. Moreover, human physiology (as well as most other physiologies to our knowledge) is quite capable of dealing with reactive oxygen species so long they do not exceed a certain threshold.
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''What would happen if one or several bioparts change their function or stop working as intended (e.g. through mutation)? How would the whole device or system change its properties and what unintended effects would result thereof?''
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Mutation of our BioBrick parts would most likely result in loss of function. Thus, our organisms would either go blind, or stop glowing. Should the extraordinary happen and gain of function occur, we fathom our organisms would be over-taxed and they would die. Since our parts are only a net drag on the survivability of our organisms, it would require some extreme mutations to render them a selective advantage for the host. However, this risk exists for any exogenous protein in a host.
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''What unintended effects could you foresee after your engineered organism is released to the environment?''
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We can foresee the spread of the BioBrick parts through the environmental bacterial populations via horizontal gene transfer. However, since the bearing plasmid only holds a selective advantage under antibiotic conditions, we expect it to be quickly lost. Moreover, in the event our devices escape the original plasmid and hop into another structure, light communication holds no selective advantage in and of itself within current free-living populations of E. coli. Thus we feel confident our devices would over-tax their hosts and ensure death.
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''Try to think outside the box, what is the absolut worst case scenario for human health or the environment, that you could imagine?''
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The Absolute Worst Case Scenario? As if in a movie by Micheal Bay? Well...<html><br /></html>
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We can image our E. colis forming a structure much like a neural network to process information as a colony instead of as a single cell. Under quite permissive conditions, they might gain a selective advantage by operating in a multi-cellular organization. If grown to 1.2 optical density in a 1 liter beaker, they may very well have more connections than a human brain. Thus, they may pose a danger to Human supremacy of the Planet!!!
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==='''Documentation & Management of Safety Issues'''===
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===='''Parts'''====
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====='''Current'''=====
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Work in progress.
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====='''Emergent'''=====
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Work in progress.
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===='''Devices & Systems'''====
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====='''Current'''=====
 +
 
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Work in progress.
 +
 
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====='''Emergent'''=====
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Work in progress.
 +
 
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===='''Cell Chassis Enhancement'''====
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====='''Current'''=====
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Work in progress.
 +
 
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====='''Emergent'''=====
 +
 
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Work in progress.
 +
 
 +
 
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The Safety page is this one: [[Safety | safety page]].

Revision as of 23:10, 15 July 2010

Contents

Safety

Identifying Safety Issues

Probability

Could there be an unplanned event or series of events involving your project, resulting in either death, injury, occupational illness, damage to equipment or property, or damage to the environment? How likely is that going to happen?

There could always be some sinister series of unfortunate events that leads to disaster, however given the nature of our project, we believe that that little beyond minimal environmental exposure is the scope of catastrophe. Our BioBricks parts are mainly enzymes. These enzymes catalyze reactions that are already found in nature in a wide variety of environmental niches. As such, they are nothing new. Nature has found ways of balancing their actions, so even if they were to be exposed dramatically, the perturbation should be minimal and easily self-correcting.


Does your project require the exposure or release of the engineered organism to people or the environment (e.g. as medicine, for bioremediation)?

Our project does not require exposure or release to either people or environment. In fact, this project is meant to be tested under extreme biological, chemical and even thermal isolation. One of the points we want to prove is the fact that we can communicate information where there is no possible biological, chemical or even thermal bridge spanning our communicating entities. They will be isolated, both from each other as from the environment.

Hazard

Could your device, when working properly, represent a hazard to people or the environment?

We do not believe our device represents a hazard. If working properly, our devices will stress the host organism without generating any short-term benefit. As such, they are only an added metabolic process to the host. At most, our devices would generate a higher-than-average amount of reactive oxygen species, but not in a significant manner. Perhaps, should the reactions be extreme in number, the light emission could be a hazard to the naked eye...


Is your engineered organism infectious? Does it produce a toxic product? Does it interfere with human physiology or the environment?

Our engineered organism is not infectious, nor does it produce toxic products. The only dangerous product of our devices are reactive oxygen species, however their net production is not significant. Moreover, human physiology (as well as most other physiologies to our knowledge) is quite capable of dealing with reactive oxygen species so long they do not exceed a certain threshold.


What would happen if one or several bioparts change their function or stop working as intended (e.g. through mutation)? How would the whole device or system change its properties and what unintended effects would result thereof?

Mutation of our BioBrick parts would most likely result in loss of function. Thus, our organisms would either go blind, or stop glowing. Should the extraordinary happen and gain of function occur, we fathom our organisms would be over-taxed and they would die. Since our parts are only a net drag on the survivability of our organisms, it would require some extreme mutations to render them a selective advantage for the host. However, this risk exists for any exogenous protein in a host.


What unintended effects could you foresee after your engineered organism is released to the environment?

We can foresee the spread of the BioBrick parts through the environmental bacterial populations via horizontal gene transfer. However, since the bearing plasmid only holds a selective advantage under antibiotic conditions, we expect it to be quickly lost. Moreover, in the event our devices escape the original plasmid and hop into another structure, light communication holds no selective advantage in and of itself within current free-living populations of E. coli. Thus we feel confident our devices would over-tax their hosts and ensure death.


Try to think outside the box, what is the absolut worst case scenario for human health or the environment, that you could imagine?

The Absolute Worst Case Scenario? As if in a movie by Micheal Bay? Well...
We can image our E. colis forming a structure much like a neural network to process information as a colony instead of as a single cell. Under quite permissive conditions, they might gain a selective advantage by operating in a multi-cellular organization. If grown to 1.2 optical density in a 1 liter beaker, they may very well have more connections than a human brain. Thus, they may pose a danger to Human supremacy of the Planet!!!

Documentation & Management of Safety Issues

Parts

Current

Work in progress.

Emergent

Work in progress.

Devices & Systems

Current

Work in progress.

Emergent

Work in progress.

Cell Chassis Enhancement

Current

Work in progress.

Emergent

Work in progress.


The Safety page is this one: safety page.