Team:UC Davis/notebook/onpathway.html

From 2010.igem.org

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<a name="construct"></a><p class="header"><b>Construct:</b></a><p>
<a name="construct"></a><p class="header"><b>Construct:</b></a><p>
<img src="https://static.igem.org/mediawiki/2010/c/cf/Onpiecesx.jpg"><p>
<img src="https://static.igem.org/mediawiki/2010/c/cf/Onpiecesx.jpg"><p>
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<a name="stepone"></a><p class="header"><b>Step One</b></a><p>
<a name="stepone"></a><p class="header"><b>Step One</b></a><p>
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  <tr>
   <td><img src="https://static.igem.org/mediawiki/2010/d/dd/Rbsc0051x.jpg"></td>
   <td><img src="https://static.igem.org/mediawiki/2010/d/dd/Rbsc0051x.jpg"></td>
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  </tr>
  </tr>
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   <td><img src="https://static.igem.org/mediawiki/2010/3/3f/Rbse1010x.jpg"></td>
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<a name="steptwo"></a><p class="header"><b>Step Two</b></a><p>
<a name="steptwo"></a><p class="header"><b>Step Two</b></a><p>
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<td width="350px"><img src="https://static.igem.org/mediawiki/2010/f/f8/Rbse1010f1610.jpg"></td>
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<td>
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  <ul>
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  <li>RBS-RFP and F1610 are ligated together</li>
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  </ul>
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</td>
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</tr>
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</table><p><br />
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<a name="stepthree"></a><p class="header"><b>Step Three</b></a><p>
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   <td><img src="https://static.igem.org/mediawiki/2010/e/e1/Rbsc0051rbse1010.jpg"></td>
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   <td>
   <td>
     <ul>
     <ul>
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     <li>RBS-C0051 and RBS-RFP are ligated together</li>
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     <li>RBS-C0051 and RBS-RFP-F1610 are ligated together</li>
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     <li>This step is where we have discovered bizarre transcription!!!</li>
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     <li><a href="https://2010.igem.org/Team:UC_Davis/notebook/c0051debug.html" class="help">This step is where we have discovered bizarre transcription!!!</a></li>
     </ul>
     </ul>
   </td>
   </td>
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</tr>
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<a name="stepfour"></a><p class="header"><b>Step Four</b></a><p>
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<table border=0 margin="0" width="600px" padding:0 margin:0>
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<tr>
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  <td width="350px"><img src="https://static.igem.org/mediawiki/2010/2/2d/Rbsc0051rbse1010f1610.jpg"></td>
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  <td><ul><li>AFTER getting rid of the C0051 bar code, we have ligated the MODIFIED c0051 to the RBS-RFP-F1610.</li></ul>
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</tr>
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</table>
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<a name="stepfive"></a><p class="header"><b>Step Five</b></a><p>
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<table border=0 margin="0" width="600px" padding:0 margin:0>
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<tr>
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  <td width="350px"><img src="https://static.igem.org/mediawiki/2010/8/8a/Onpiecescomplete.jpg"></td>
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  <td><ul><li>After ligating the piece to the I12006 promoter, we have now completed the ON switch!</li></ul></td>
  </tr>
  </tr>
</table>
</table>
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<li><a href="#stepone" class="help">Step One</a></li>
<li><a href="#stepone" class="help">Step One</a></li>
<li><a href="#steptwo" class="help">Step Two</a></li>
<li><a href="#steptwo" class="help">Step Two</a></li>
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<li><a href="#stepthree" class="help">Step Three</a></li>
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<li><a href="#stepfour" class="help">Step Four</a></li>
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<li><a href="#stepfive" class="help">Step Five</a></li>
</ul> </td>
</ul> </td>
               </tr>
               </tr>

Latest revision as of 03:33, 28 October 2010

ON pathway

Construct:

Step One

  • B0034 is ligated to both C0051 and E1010 (also known as RFP)

Step Two

  • RBS-RFP and F1610 are ligated together


Step Three

Step Four

  • AFTER getting rid of the C0051 bar code, we have ligated the MODIFIED c0051 to the RBS-RFP-F1610.

Step Five

  • After ligating the piece to the I12006 promoter, we have now completed the ON switch!

We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)

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