http://2010.igem.org/wiki/index.php?title=Team:TzuChiU_Formosa/Safety&feed=atom&action=history
Team:TzuChiU Formosa/Safety - Revision history
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Sumi at 07:14, 4 December 2010
2010-12-04T07:14:45Z
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Sumi
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Sumi at 03:36, 28 October 2010
2010-10-28T03:36:01Z
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Sumi
http://2010.igem.org/wiki/index.php?title=Team:TzuChiU_Formosa/Safety&diff=205149&oldid=prev
Nichun at 02:15, 28 October 2010
2010-10-28T02:15:18Z
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Nichun
http://2010.igem.org/wiki/index.php?title=Team:TzuChiU_Formosa/Safety&diff=203301&oldid=prev
Nichun at 01:02, 28 October 2010
2010-10-28T01:02:19Z
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Nichun
http://2010.igem.org/wiki/index.php?title=Team:TzuChiU_Formosa/Safety&diff=178384&oldid=prev
Nichun at 10:07, 27 October 2010
2010-10-27T10:07:22Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h2>Q1. Would any of your project ideas raise safety issues in terms of: <br /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h2<ins class="diffchange diffchange-inline">><strong</ins>>Q1. Would any of your project ideas raise safety issues in terms of: <br /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> researcher safety, public safety, or: environmental safety? </h2></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> researcher safety, public safety, or: environmental safety? <ins class="diffchange diffchange-inline"></strong></ins></h2></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>We only used <em>E. coli</em> DH5α in all our experiments when required. DH5α is not pathogenic to humans nor animals and will not survive in the environment. The inserted sequences encode only plant enzymes which is necessary to made β-carotene and are highly unlikely to confer any harmful phenotype on the disabled <em>E.coli</em> host nor animals. Standard good lab practices were followed strictly in handling all bacterial samples, including wearing protective groves, working in lamina flow, autoclaving contaminated waste, bleaching discarded liquid cultures, and washing hands before and after experiments.</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>We only used <em>E. coli</em> DH5α in all our experiments when required. DH5α is not pathogenic to humans nor animals and will not survive in the environment. The inserted sequences encode only plant enzymes which is necessary to made β-carotene and are highly unlikely to confer any harmful phenotype on the disabled <em>E.coli</em> host nor animals. Standard good lab practices were followed strictly in handling all bacterial samples, including wearing protective groves, working in lamina flow, autoclaving contaminated waste, bleaching discarded liquid cultures, and washing hands before and after experiments.</h3></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h2>Q2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, <br /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h2<ins class="diffchange diffchange-inline">><strong</ins>>Q2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, <br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> did you document these issues in the Registry? <br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> did you document these issues in the Registry? <br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> how did you manage to handle the safety issue? <br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> how did you manage to handle the safety issue? <br /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> how could other teams learn from your experience?</h2></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> how could other teams learn from your experience?<ins class="diffchange diffchange-inline"></strong></ins></h2></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h2>Q3. Is there a local biosafety group, committee, or review board at your institution? <br /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h2<ins class="diffchange diffchange-inline">><strong</ins>>Q3. Is there a local biosafety group, committee, or review board at your institution? <br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> If yes, what does your local biosafety group think about your project? <br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> If yes, what does your local biosafety group think about your project? <br /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> If no, which specific biosafety rules or guidelines do you have to consider in your country?</h2></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> If no, which specific biosafety rules or guidelines do you have to consider in your country?<ins class="diffchange diffchange-inline"></strong></ins></h2></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="<del class="diffchange diffchange-inline">203</del>" valign="top"><blockquote></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="<ins class="diffchange diffchange-inline">156</ins>" valign="top"><blockquote></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Yes. All bacterial or plasmid used have to obtain an approval from University's environmental and biosafety committees. Environmental and biosafety officers made regular random visit to each laboratory to ensure all laboratories are comply with environmental and biosafety regulations. So far our project has received positive responds from the committees.</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Yes. All bacterial or plasmid used have to obtain an approval from University's environmental and biosafety committees. Environmental and biosafety officers made regular random visit to each laboratory to ensure all laboratories are comply with environmental and biosafety regulations. So far our project has received positive responds from the committees.</h3></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h2>Q4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </h2></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h2<ins class="diffchange diffchange-inline">><strong</ins>>Q4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? <ins class="diffchange diffchange-inline"></strong></ins></h2></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Perhaps parts, devices or systems can be divided into two categories such as prokaryotes and eukaryotes and regulate under standard environmental and biosafety regulations.</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3>Perhaps parts, devices or systems can be divided into two categories such as prokaryotes and eukaryotes and regulate under standard environmental and biosafety regulations.</h3></div></td></tr>
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Nichun
http://2010.igem.org/wiki/index.php?title=Team:TzuChiU_Formosa/Safety&diff=178299&oldid=prev
Nichun at 10:02, 27 October 2010
2010-10-27T10:02:03Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 10:02, 27 October 2010</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><blockquote>&<del class="diffchange diffchange-inline">nbsp</del>;</blockquote></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><blockquote></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <h2>Safety Q</ins>&<ins class="diffchange diffchange-inline">amp</ins>;<ins class="diffchange diffchange-inline">A</h2></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <h3>For iGEM 2010 we are asked to detail how we approached any issues of biological safety </h3></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <h3>associated with their projects (<a href="https://2010.igem.org/Team:TzuChiU_Formosa/Nutrient_synthesizer">see here</a>). Here are the questions and our answers:</h3></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></blockquote></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="26" valign="top" bgcolor="#FFDDAC"><h2><del class="diffchange diffchange-inline">Researcher </del> safety</h2></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="26" valign="top" bgcolor="#FFDDAC"><ins class="diffchange diffchange-inline"><blockquote></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><h2><ins class="diffchange diffchange-inline">Q1. Would any of your project ideas raise </ins> safety <ins class="diffchange diffchange-inline">issues in terms of: <br /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> researcher safety, public safety, or: environmental safety? </ins></h2<ins class="diffchange diffchange-inline">></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </blockquote</ins>></td></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="169" valign="top"><<del class="diffchange diffchange-inline">h3</del>><<del class="diffchange diffchange-inline">strong</del>><del class="diffchange diffchange-inline">In our experiment, we </del>used <em>E. coli</em><del class="diffchange diffchange-inline">&nbsp;</del>DH5α <del class="diffchange diffchange-inline"> as </del>our <del class="diffchange diffchange-inline">model to drive our experimental design </del>DH5α <del class="diffchange diffchange-inline">was a well know </del><em>E. coli</em> <del class="diffchange diffchange-inline">strain to be harmless</del>. <del class="diffchange diffchange-inline">In addition, the genes </del> <del class="diffchange diffchange-inline">we constructed </del>were all <del class="diffchange diffchange-inline">harmless to human being. A well trained researcher would be safe with basic </del> <del class="diffchange diffchange-inline">protection</del>. </<del class="diffchange diffchange-inline">strong</del>></<del class="diffchange diffchange-inline">h3</del>></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="169" valign="top"><<ins class="diffchange diffchange-inline">blockquote</ins>></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><<ins class="diffchange diffchange-inline">h3</ins>><ins class="diffchange diffchange-inline">We only </ins>used <em>E. coli</em> DH5α <ins class="diffchange diffchange-inline">in all </ins>our <ins class="diffchange diffchange-inline">experiments when required. </ins>DH5α <ins class="diffchange diffchange-inline"> is not pathogenic to humans nor animals and will not survive in the environment. The inserted sequences encode only plant enzymes which is necessary to made β-carotene and are highly unlikely to confer any harmful phenotype on the disabled </ins><em>E.coli</em> <ins class="diffchange diffchange-inline">host nor animals</ins>. <ins class="diffchange diffchange-inline">Standard </ins> <ins class="diffchange diffchange-inline">good lab practices </ins>were <ins class="diffchange diffchange-inline">followed strictly in handling </ins>all <ins class="diffchange diffchange-inline">bacterial samples, including wearing protective groves, working in lamina flow, autoclaving contaminated waste, bleaching discarded liquid cultures, and washing hands </ins> <ins class="diffchange diffchange-inline">before and after experiments</ins>.</<ins class="diffchange diffchange-inline">h3</ins>></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></<ins class="diffchange diffchange-inline">blockquote></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <p>&nbsp;</p</ins>></td></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td bgcolor="#FFDDAD"><h2><del class="diffchange diffchange-inline">Public </del> safety</h2></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td bgcolor="#FFDDAD"><ins class="diffchange diffchange-inline"><blockquote></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><h2><ins class="diffchange diffchange-inline">Q2. Do any of the new BioBrick parts (or devices) </ins> <ins class="diffchange diffchange-inline">that you made this year raise any </ins>safety <ins class="diffchange diffchange-inline">issues? If yes, <br /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> did you document these issues in the Registry? <br /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> how did you manage to handle the safety issue? <br /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> how could other teams learn from your experience?</ins></h2<ins class="diffchange diffchange-inline">></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </blockquote</ins>></td></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="168" valign="top"><<del class="diffchange diffchange-inline">h3</del>><<del class="diffchange diffchange-inline">strong</del>><del class="diffchange diffchange-inline"><em>E</del>. <del class="diffchange diffchange-inline"> coli</del></<del class="diffchange diffchange-inline">em</del>> <del class="diffchange diffchange-inline">strain transfected with constructed genes would not be lysed and produced harmful products under normal situation. </del></<del class="diffchange diffchange-inline">strong></h3</del>></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="168" valign="top"><<ins class="diffchange diffchange-inline">blockquote</ins>></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><<ins class="diffchange diffchange-inline">h3</ins>><ins class="diffchange diffchange-inline">No</ins>.</<ins class="diffchange diffchange-inline">h3</ins>></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></<ins class="diffchange diffchange-inline">blockquote</ins>></td></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="28" valign="top" bgcolor="#FFDDAD"><h2><del class="diffchange diffchange-inline">Environmental </del> <del class="diffchange diffchange-inline">safety </del>?</h2></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="28" valign="top" bgcolor="#FFDDAD"><ins class="diffchange diffchange-inline"><blockquote></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><h2><ins class="diffchange diffchange-inline">Q3. Is there a local biosafety group, committee, </ins> <ins class="diffchange diffchange-inline">or review board at your institution? <br /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> If yes, what does your local biosafety group think about your project? <br /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> If no, which specific biosafety rules or guidelines do you have to consider in your country</ins>?</h2<ins class="diffchange diffchange-inline">></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </blockquote</ins>></td></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="203" valign="top"><<del class="diffchange diffchange-inline">h3</del>><<del class="diffchange diffchange-inline">strong</del>><del class="diffchange diffchange-inline">Our </del> <del class="diffchange diffchange-inline">device was to create nutrient, </del>and <del class="diffchange diffchange-inline">the structure of the final product was equal </del> to <del class="diffchange diffchange-inline">the nature compound, so it will not damage environment </del>with <del class="diffchange diffchange-inline">the nutrient </del> <del class="diffchange diffchange-inline">that already present in nature</del>. <del class="diffchange diffchange-inline">The enzyme we produced, CrtEBIY, Holin, </del> <del class="diffchange diffchange-inline">Lysozyme, were present in </del>the <del class="diffchange diffchange-inline">environment, and need to be produced by induction , Therefore, it can not be made in the natural environment</del>.</<del class="diffchange diffchange-inline">strong</del>></<del class="diffchange diffchange-inline">h3</del>></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="203" valign="top"><<ins class="diffchange diffchange-inline">blockquote</ins>></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><<ins class="diffchange diffchange-inline">h3</ins>><ins class="diffchange diffchange-inline">Yes. All bacterial or plasmid used have to </ins> <ins class="diffchange diffchange-inline">obtain an approval from University's environmental </ins>and <ins class="diffchange diffchange-inline">biosafety committees. </ins> <ins class="diffchange diffchange-inline">Environmental and biosafety officers made regular random visit </ins>to <ins class="diffchange diffchange-inline">each laboratory to ensure all laboratories are comply </ins>with <ins class="diffchange diffchange-inline">environmental and </ins> <ins class="diffchange diffchange-inline">biosafety regulations</ins>. <ins class="diffchange diffchange-inline">So far our project has received positive responds from </ins> the <ins class="diffchange diffchange-inline">committees</ins>.</<ins class="diffchange diffchange-inline">h3</ins>></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></<ins class="diffchange diffchange-inline">blockquote</ins>></td></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="33" valign="top" bgcolor="#FFDDAD"><<del class="diffchange diffchange-inline">h2</del>><<del class="diffchange diffchange-inline">strong</del>><del class="diffchange diffchange-inline">Protection of Research:</del></<del class="diffchange diffchange-inline">strong</del>></<del class="diffchange diffchange-inline">h2</del>></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="33" valign="top" bgcolor="#FFDDAD"><<ins class="diffchange diffchange-inline">blockquote</ins>></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><<ins class="diffchange diffchange-inline">h2</ins>><ins class="diffchange diffchange-inline">Q4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </ins></<ins class="diffchange diffchange-inline">h2</ins>></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></<ins class="diffchange diffchange-inline">blockquote</ins>></td></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="203" valign="top"><<del class="diffchange diffchange-inline">h3>1. <strong>Every researcher must be well trained before carrying out experiment.</strong></h3</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="203" valign="top"><<ins class="diffchange diffchange-inline">blockquote</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <h3><del class="diffchange diffchange-inline"><strong>2. Before join the iGem team</del>, <del class="diffchange diffchange-inline">everyone need </del> <del class="diffchange diffchange-inline">to attend the biosafety training lesson.</strong></h3></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <h3><ins class="diffchange diffchange-inline">Perhaps parts</ins>, <ins class="diffchange diffchange-inline">devices or systems can be divided </ins> <ins class="diffchange diffchange-inline">into two categories such as prokaryotes and eukaryotes and regulate under </ins> <ins class="diffchange diffchange-inline">standard environmental </ins>and <ins class="diffchange diffchange-inline">biosafety regulations</ins>.</h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <h3><strong>3. When performing experiments, all </del> <del class="diffchange diffchange-inline">researchers must wear gloves, clinical masks, laboratory </strong><strong>clothing, </del>and <del class="diffchange diffchange-inline">goggles</del>.<del class="diffchange diffchange-inline"></strong></del></h3<del class="diffchange diffchange-inline">></td></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </<ins class="diffchange diffchange-inline">blockquote</ins>></td></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </tr></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <tr</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline"><td height="33" valign="top" bgcolor="#FFDDAD"><h2><strong>Biosafety:</del></<del class="diffchange diffchange-inline">strong><strong> </strong></h2></td></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </tr></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <tr></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <td height="164" valign="top"><h3><strong>Nutrient synthesizer aims to be applied to human body. Therefore, bio-safety for human practice is highly concerned. Since microbe will secrete biofilm that harm human body. In order to misgivings of microbe safety, we create a system may lysis the microbe by itself that decreased the hazardous of our device. </strong></h3</del>></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td></tr>
</table>
Nichun
http://2010.igem.org/wiki/index.php?title=Team:TzuChiU_Formosa/Safety&diff=168104&oldid=prev
Sumi at 22:45, 26 October 2010
2010-10-26T22:45:28Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 22:45, 26 October 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <td bgcolor="#FFDDAD"><h2>Public safety</h2></td></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <td bgcolor="#FFDDAD"><h2>Public safety</h2></td></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <tr></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <td height="168" valign="top"><h3><strong><em>E. coli</em> strain transfected with constructed genes would not be lysed and produced harmful products under normal situation. </strong></h3></td></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </tr></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <tr></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <td height="28" valign="top" bgcolor="#FFDDAD"><h2>Environmental safety ?</h2></td></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </tr></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <tr></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <td height="203" valign="top"><h3><strong>Our device was to create nutrient, and the structure of the final product was equal to the nature compound, so it will not damage environment with the nutrient that already present in nature. The enzyme we produced, CrtEBIY, Holin, Lysozyme, were present in the environment, and need to be produced by induction , Therefore, it can not be made in the natural environment.</strong></h3></td></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </tr></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <tr></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <td height="33" valign="top" bgcolor="#FFDDAD"><h2><strong>Protection of Research:</strong></h2></td></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </tr></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <tr></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <td height="203" valign="top"><h3>1. <strong>Every researcher must be well trained before carrying out experiment.</strong></h3></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <h3><strong>2. Before join the iGem team, everyone need to attend the biosafety training lesson.</strong></h3></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <h3><strong>3. When performing experiments, all researchers must wear gloves, clinical masks, laboratory </strong><strong>clothing, and goggles.</strong></h3></td></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </tr></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <tr></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <td height="33" valign="top" bgcolor="#FFDDAD"><h2><strong>Biosafety:</strong><strong> </strong></h2></td></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </tr></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <tr></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <td height="164" valign="top"><h3><strong>Nutrient synthesizer aims to be applied to human body. Therefore, bio-safety for human practice is highly concerned. Since microbe will secrete biofilm that harm human body. In order to misgivings of microbe safety, we create a system may lysis the microbe by itself that decreased the hazardous of our device. </strong></h3></td></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </tr></ins></div></td></tr>
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</table>
Sumi
http://2010.igem.org/wiki/index.php?title=Team:TzuChiU_Formosa/Safety&diff=168090&oldid=prev
Sumi at 22:44, 26 October 2010
2010-10-26T22:44:49Z
<p></p>
<table style="background-color: white; color:black;">
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<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 22:44, 26 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 28:</td>
<td colspan="2" class="diff-lineno">Line 28:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <td bgcolor="#FFDDAD"><h2>Public safety</h2></td></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <td bgcolor="#FFDDAD"><h2>Public safety</h2></td></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <tr></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <td height="168" valign="top"><h3><strong><em>E. coli</em> strain transfected with constructed genes would not be lysed and produced harmful products under normal situation. </strong></h3></td></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </tr></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <tr></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <td height="28" valign="top" bgcolor="#FFDDAD"><h2>Environmental safety ?</h2></td></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </tr></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <tr></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <td height="203" valign="top"><h3><strong>Our device was to create nutrient, and the structure of the final product was equal to the nature compound, so it will not damage environment with the nutrient that already present in nature. The enzyme we produced, CrtEBIY, Holin, Lysozyme, were present in the environment, and need to be produced by induction , Therefore, it can not be made in the natural environment.</strong></h3></td></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </tr></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <tr></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <td height="33" valign="top" bgcolor="#FFDDAD"><h2><strong>Protection of Research:</strong></h2></td></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </tr></del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h3><strong>Every researcher must be well trained before carrying out experiment. </strong></h3></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h3><strong>Before join the iGem team, everyone need to attend the biosafety training lesson.</strong></h3></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <h3><strong>When performing experiments, all researchers must wear gloves, clinical masks, laboratory clothing, and goggles.</strong></h3></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </ol></td></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </tr></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <tr></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <td height="33" valign="top" bgcolor="#FFDDAD"><h2><strong>Biosafety:</strong><strong> </strong></h2></td></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </tr></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <tr></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <td height="164" valign="top"><h3><strong>Nutrient synthesizer aims to be applied to human body. Therefore, bio-safety for human practice is highly concerned. Since microbe will secrete biofilm that harm human body. In order to misgivings of microbe safety, we create a system may lysis the microbe by itself that decreased the hazardous of our device. </strong></h3></td></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </tr></del></div></td><td colspan="2"> </td></tr>
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Sumi
http://2010.igem.org/wiki/index.php?title=Team:TzuChiU_Formosa/Safety&diff=168050&oldid=prev
Sumi at 22:42, 26 October 2010
2010-10-26T22:42:00Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="26" valign="top" bgcolor="#<del class="diffchange diffchange-inline">FFDDAD</del>"><<del class="diffchange diffchange-inline">h3</del>>Researcher safety</<del class="diffchange diffchange-inline">h3</del>></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="26" valign="top" bgcolor="#<ins class="diffchange diffchange-inline">FFDDAC</ins>"><<ins class="diffchange diffchange-inline">h2</ins>>Researcher safety</<ins class="diffchange diffchange-inline">h2</ins>></td></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="169" valign="top"><<del class="diffchange diffchange-inline">p</del>><strong>In our experiment, we used <em>E. coli</em>&nbsp;DH5α as our model to drive our experimental design DH5α was a well know E<del class="diffchange diffchange-inline">-</del>coli strain to be harmless. In addition, the genes we constructed were all harmless to human being. A well trained researcher would be safe with basic protection. </strong></<del class="diffchange diffchange-inline">p</del>></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="169" valign="top"><<ins class="diffchange diffchange-inline">h3</ins>><strong>In our experiment, we used <em>E. coli</em>&nbsp;DH5α as our model to drive our experimental design DH5α was a well know <ins class="diffchange diffchange-inline"><em></ins>E<ins class="diffchange diffchange-inline">. </ins>coli<ins class="diffchange diffchange-inline"></em> </ins>strain to be harmless. In addition, the genes we constructed were all harmless to human being. A well trained researcher would be safe with basic protection. </strong></<ins class="diffchange diffchange-inline">h3</ins>></td></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td bgcolor="#FFDDAD"><<del class="diffchange diffchange-inline">h3</del>>Public safety</<del class="diffchange diffchange-inline">h3</del>></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td bgcolor="#FFDDAD"><<ins class="diffchange diffchange-inline">h2</ins>>Public safety</<ins class="diffchange diffchange-inline">h2</ins>></td></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="168" valign="top"><<del class="diffchange diffchange-inline">p</del>><strong>E. coli strain transfected with constructed genes would not be lysed and produced harmful products under normal situation. </strong></<del class="diffchange diffchange-inline">p</del>></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="168" valign="top"><<ins class="diffchange diffchange-inline">h3</ins>><strong<ins class="diffchange diffchange-inline">><em</ins>>E. coli<ins class="diffchange diffchange-inline"></em> </ins>strain transfected with constructed genes would not be lysed and produced harmful products under normal situation. </strong></<ins class="diffchange diffchange-inline">h3</ins>></td></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="28" valign="top" bgcolor="#FFDDAD"><<del class="diffchange diffchange-inline">h3</del>>Environmental safety ?</<del class="diffchange diffchange-inline">h3</del>></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="28" valign="top" bgcolor="#FFDDAD"><<ins class="diffchange diffchange-inline">h2</ins>>Environmental safety ?</<ins class="diffchange diffchange-inline">h2</ins>></td></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="203" valign="top"><<del class="diffchange diffchange-inline">p</del>><strong>Our device was to create nutrient, and the structure of the final product was equal to the nature compound, so it will not damage environment with the nutrient that already present in nature. The enzyme we produced, CrtEBIY, Holin, Lysozyme, were present in the environment, and need to be produced by induction , Therefore, it can not be made in the natural environment.</strong></<del class="diffchange diffchange-inline">p</del>></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="203" valign="top"><<ins class="diffchange diffchange-inline">h3</ins>><strong>Our device was to create nutrient, and the structure of the final product was equal to the nature compound, so it will not damage environment with the nutrient that already present in nature. The enzyme we produced, CrtEBIY, Holin, Lysozyme, were present in the environment, and need to be produced by induction , Therefore, it can not be made in the natural environment.</strong></<ins class="diffchange diffchange-inline">h3</ins>></td></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="33" valign="top" bgcolor="#FFDDAD"><<del class="diffchange diffchange-inline">h3</del>><strong>Protection of Research:</strong></<del class="diffchange diffchange-inline">h3</del>></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="33" valign="top" bgcolor="#FFDDAD"><<ins class="diffchange diffchange-inline">h2</ins>><strong>Protection of Research:</strong></<ins class="diffchange diffchange-inline">h2</ins>></td></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><strong>Every researcher must be well trained before carrying out experiment. </strong></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li<ins class="diffchange diffchange-inline">></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><strong>Before join the iGem team, everyone need to attend the biosafety training lesson.</strong></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <h3</ins>><strong>Every researcher must be well trained before carrying out experiment. </strong><ins class="diffchange diffchange-inline"></h3></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><strong>When performing experiments, all researchers must wear gloves, clinical masks, laboratory clothing, and goggles</strong></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></li></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <h3</ins>><strong>Before join the iGem team, everyone need to attend the biosafety training lesson.</strong><ins class="diffchange diffchange-inline"></h3></ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="164" valign="top"><<del class="diffchange diffchange-inline">p</del>><strong>Nutrient synthesizer aims to be applied to human body. Therefore, bio-safety for human practice is highly concerned. Since microbe will secrete biofilm that harm human body. In order to misgivings of microbe safety, we create a system may lysis the microbe by itself that decreased the hazardous of our device. </strong></<del class="diffchange diffchange-inline">p</del>></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="164" valign="top"><<ins class="diffchange diffchange-inline">h3</ins>><strong>Nutrient synthesizer aims to be applied to human body. Therefore, bio-safety for human practice is highly concerned. Since microbe will secrete biofilm that harm human body. In order to misgivings of microbe safety, we create a system may lysis the microbe by itself that decreased the hazardous of our device. </strong></<ins class="diffchange diffchange-inline">h3</ins>></td></div></td></tr>
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Sumi
http://2010.igem.org/wiki/index.php?title=Team:TzuChiU_Formosa/Safety&diff=166036&oldid=prev
Sumi at 20:34, 26 October 2010
2010-10-26T20:34:59Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td height="<del class="diffchange diffchange-inline">39</del>" valign="top" bgcolor="#<del class="diffchange diffchange-inline">ff9900</del>"><h3><strong>Biosafety:</strong><strong> </strong></h3></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td height="<ins class="diffchange diffchange-inline">33</ins>" valign="top" bgcolor="#<ins class="diffchange diffchange-inline">FFDDAD</ins>"><h3><strong>Biosafety:</strong><strong> </strong></h3></td></div></td></tr>
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