Team:TzuChiU Formosa/Safety

From 2010.igem.org

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  <h2>Safety Q&amp;A</h2>
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  <h3>For iGEM 2010 we are asked to detail how we  approached any issues of biological safety </h3>
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  <h3>associated with their projects (<a href="https://2010.igem.org/Team:TzuChiU_Formosa/Nutrient_synthesizer">see here</a>).  Here are the questions and our answers:</h3>
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     <td height="26" valign="top" bgcolor="#FFDDAC"><h2>Researcher safety</h2></td>
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      <h2>Q1. Would any of your project ideas raise safety issues in terms of: <br />
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        researcher safety, public safety, or: environmental safety? </h2>
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     <td height="169" valign="top"><h3><strong>In  our experiment, we used <em>E. coli</em>&nbsp;DH5α as our model to drive our experimental design DH5α was a well know <em>E. coli</em> strain to be harmless. In addition, the genes we constructed were all harmless to human being. A well trained researcher would be safe with basic protection. </strong></h3></td>
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      <h3>We only used <em>E. coli</em> DH5α in all our experiments when required. DH5α is not pathogenic to humans nor animals and will not survive in the  environment. The inserted sequences encode only plant enzymes which is  necessary to made β-carotene and are highly unlikely to  confer any harmful phenotype on the disabled <em>E.coli</em> host nor animals. Standard good lab practices were followed strictly in handling all bacterial samples,  including wearing protective groves, working in lamina flow, autoclaving  contaminated waste, bleaching discarded liquid cultures, and washing hands before and after experiments.</h3>
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     <td bgcolor="#FFDDAD"><h2>Public safety</h2></td>
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      <h2>Q2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, <br />
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        did you document these issues in the Registry? <br />
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        how did you manage to handle the safety  issue? <br />
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        how could other teams learn from your experience?</h2>
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     <td height="168" valign="top"><h3><strong><em>E. coli</em> strain transfected with constructed genes would not be lysed and produced  harmful products under normal situation. </strong></h3></td>
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      <h3>No.</h3>
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     <td height="28" valign="top" bgcolor="#FFDDAD"><h2>Environmental safety ?</h2></td>
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      <h2>Q3. Is there a local biosafety group, committee, or review board at your institution? <br />
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        If yes, what does your local biosafety group  think about your project? <br />
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        If no, which specific biosafety rules or  guidelines do you have to consider in your country?</h2>
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     <td height="203" valign="top"><h3><strong>Our device was to create nutrient, and the structure of the final product was equal to the nature compound, so it will not damage environment with the nutrient that already present in nature. The enzyme we produced, CrtEBIY, Holin, Lysozyme, were present in the environment, and need to be produced by induction  , Therefore, it can not be made in the natural environment.</strong></h3></td>
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      <h3>Yes. All bacterial or plasmid used have to obtain an approval from University's environmental and biosafety committees. Environmental and biosafety officers made regular random visit to each  laboratory to ensure all laboratories are comply with environmental and biosafety regulations. So far our project has received positive responds from the committees.</h3>
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     <td height="33" valign="top" bgcolor="#FFDDAD"><h2><strong>Protection of Research:</strong></h2></td>
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      <h2>Q4. Do you have any other ideas how to deal with  safety issues that could be useful for future iGEM competitions? How could  parts, devices and systems be made even safer through biosafety engineering? </h2>
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     <td height="203" valign="top"><h3>1. <strong>Every researcher must be well trained  before carrying out experiment.</strong></h3>
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       <h3><strong>2. Before join the iGem team, everyone need to attend the biosafety training lesson.</strong></h3>
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       <h3>Perhaps parts, devices or systems can be divided into two categories such as prokaryotes and eukaryotes and regulate under standard environmental and biosafety regulations.</h3>
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    <h3><strong>3. When performing experiments, all researchers must wear gloves, clinical masks, laboratory </strong><strong>clothing, and goggles.</strong></h3></td>
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     <td height="33" valign="top" bgcolor="#FFDDAD"><h2><strong>Biosafety:</strong><strong> </strong></h2></td>
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    <td height="164" valign="top"><h3><strong>Nutrient synthesizer aims to be applied  to human body. Therefore, bio-safety for human practice is highly concerned. Since  microbe will secrete biofilm that harm human body. In order to misgivings of  microbe safety, we create a system may lysis the microbe by itself that  decreased the hazardous of our device. </strong></h3></td>
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Revision as of 10:02, 27 October 2010

無標題文件

Safety Q&A

For iGEM 2010 we are asked to detail how we approached any issues of biological safety

associated with their projects (see here). Here are the questions and our answers:

Q1. Would any of your project ideas raise safety issues in terms of:
researcher safety, public safety, or: environmental safety?

We only used E. coli DH5α in all our experiments when required. DH5α is not pathogenic to humans nor animals and will not survive in the environment. The inserted sequences encode only plant enzymes which is necessary to made β-carotene and are highly unlikely to confer any harmful phenotype on the disabled E.coli host nor animals. Standard good lab practices were followed strictly in handling all bacterial samples, including wearing protective groves, working in lamina flow, autoclaving contaminated waste, bleaching discarded liquid cultures, and washing hands before and after experiments.

 

Q2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,
did you document these issues in the Registry?
how did you manage to handle the safety issue?
how could other teams learn from your experience?

No.

Q3. Is there a local biosafety group, committee, or review board at your institution?
If yes, what does your local biosafety group think about your project?
If no, which specific biosafety rules or guidelines do you have to consider in your country?

Yes. All bacterial or plasmid used have to obtain an approval from University's environmental and biosafety committees. Environmental and biosafety officers made regular random visit to each laboratory to ensure all laboratories are comply with environmental and biosafety regulations. So far our project has received positive responds from the committees.

Q4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

Perhaps parts, devices or systems can be divided into two categories such as prokaryotes and eukaryotes and regulate under standard environmental and biosafety regulations.