Team:Tsinghua/experiments

From 2010.igem.org

Revision as of 17:19, 27 October 2010 by Wishyx (Talk | contribs)

Until Jamboree

days

hours

minutes

seconds

Follow us on


Visitor Locations

Join the conversation

Experiment Records

July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31

Our Experiments were carried out by the following nine groups which are somewhat independent from each other. Here come the Groups and Their Tasks.

Group 1A:Landing pad (LP) construction and Insertion with Tranditional Att Recomination method(Promoter Inside)

Group 1B:Landing pad (LP) construction and Insertion with Innovational Att Recomination method(Promoter Outside)

Group 1C:Landing pad (LP) construction and Insertion: Inducement and Recombination

Group 2A:Donor Plasmid Construction: Two Fragments Construction (Three Fragments Ligation)

Group 2B:Donor Plasmid Construction: Traditional Four Fragments Construction

Group 2C:Donor Plasmid Construction: Four Fragments Construction with Dra III

Group 2D:Donor Plasmid Construction: Four Fragments Construction with Novel Methods

Group 3:Helper Plasmid (HP) Insertion, Donor Plasmid Insertion, Recombination Induction & Removal of HP

Group 4:Screening Strategy
</div></div> <a name="prt"></a>

Protocols

Here are our Experimental Protocols in PDF version. You're advised to view in <a href="http://www.adobe.com/acom/" target=blank>Acrobat</a> or <a href="http://get.adobe.com/reader/" target=blank>Adobe Reader</a> which you can download free from the<a href="http://www.adobe.com" target=blank> Home Website of Adobe® Company</a>, for other viewers we can not guarantee the right format of viewing. Those files are encrypted to keep copyrights, if you want to print them out of copy to your documents, to share with others our ideas and methods, you are welcome to ask us for the unencrypted files definitely for free~

Molecular Cloning

<a href="http://2010.igem.org/wiki/images/0/0a/THUProtocol_1-1_Isolatio_of_plasmid_DNA.pdf" target=blank>THUProtocol_1-1_Isolatio_of_plasmid_DNA</a>

<a href="http://2010.igem.org/wiki/images/d/d8/THUProtocol_1-2_DNA_Gel_Extraction.pdf" target=blank>THUProtocol_1-2_DNA_Gel_Extraction</a>

<a href="http://2010.igem.org/wiki/images/5/59/THUProtocol_1-3_Restriction_Enzyme_Digestion.pdf" target=blank>THUProtocol_1-3_Restriction_Enzyme_Digestion</a>

<a href="http://2010.igem.org/wiki/images/b/b7/THUProtocol_1-4_Ligation_of_DNA_Fragments.pdf" target=blank>THUProtocol_1-4_Ligation_of_DNA_Fragments</a>

<a href="http://2010.igem.org/wiki/images/c/c3/THUProtocol_1-5_PCR.pdf" target=blank>THUProtocol_1-5_PCR</a>

<a href="http://2010.igem.org/wiki/images/c/c0/THUProtocol_1-6_Preparation_of_Competent_Cell_for_Electro_Transformation.pdf" target=blank>THUProtocol_1-6_Preparation_of_Competent_Cell_for_Electro_Transformation</a>

<a href="http://2010.igem.org/wiki/images/7/77/THUProtocol_1-7_Chemical_Transformation_of_Recombinant_DNA.pdf" target=blank>THUProtocol_1-7_Chemical_Transformation_of_Recombinant_DNA</a>

<a href="http://2010.igem.org/wiki/images/4/40/THUProtocol_1-8_Electro_Transformation_of_Recombinant_DNA.pdf" target=blank>THUProtocol_1-8_Electro_Transformation_of_Recombinant_DNA</a>

Protein Isolation and Identification
<a href="http://2010.igem.org/wiki/images/f/f0/THUProtocol_2-1_Protein_Isolation_for_Prokaryotes.pdf" target=blank>THUProtocol_2-1_Protein_Isolation_for_Prokaryotes</a>

<a href="http://2010.igem.org/wiki/images/0/08/THUProtocol_2-2_Protein_Identification_SDS-PAGE.pdf" target=blank>THUProtocol_2-2_Protein_Identification_SDS-PAGE</a>

<a href="http://2010.igem.org/wiki/images/c/c3/THUProtocol_1-5_PCR.pdf" target=blank>THUProtocol_1-5_PCR</a>

</div> </div></div> </body> </html>