Team:Tsinghua/experiments
From 2010.igem.org
Experiment Records
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Our Experiments were carried out by the following nine groups which are somewhat independent from each other. Here come the Groups and Their Tasks.
Group 1A:Landing pad (LP) construction and Insertion Project with Att Recomination method and Trandition Landing pad
Protocols
Molecular CloningTHU_Protocol_1-1_Isolation_of_plasmid_DNA
THU_Protocol_1-3_Restriction_Enzyme_Digestion
THU_Protocol_1-4_Ligation_of_DNA_Fragments
THU_Protocol_1-10_Electro_Transformation_of_Recombinant_DNA
THU_Protocol_1-15_PCR
Protein Isolation and Identification
Results
Group 2(b)1. PCR: Amplication eGFP, mCherry, Kanamycin resistant gene and Chlr four genes separatly from PIB-eGFP, PBS34, PKD13 and PKD3 plasmids.
2. pUC19 double digestion for eGFP and Chlr, ligate with digested eGFP and Chlr separately. PCR to detect pUC19+eGFP and pUC19+ChlrPCR. All 12 samples are positive.
3. Double digest PE1 and mCherry with SalI and BamHI, double digest PC1 and Kan with KpnI and BamHI, ligate and PCR to detect. No positive results exist among PC1+K.
4. Double digest PEM2 and Kan with KpnI and BamHI as well as PC1. Ligate and using PCR to detect with universal primers. PEM2+K 1 and 5 are positive.
5. Double digest PEMK1 and Chlr with KpnI and EcoRI. Ligate and using PCR to detect with universal primers. PEMK1+Chlr 3-5 are positive.