"
Team:Tsinghua/experiments
From 2010.igem.org
days
hours
minutes
seconds
Follow us on
Visitor Locations
Join the conversation
Experiment Records
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Our Experiments were carried out by the following nine groups which are somewhat independent from each other. Here come the Groups and Their Tasks.
Group 1A：Landing pad (LP) construction and Insertion with Tranditional Att Recomination method(Promoter Inside)
Group 1B：Landing pad (LP) construction and Insertion with Innovational Att Recomination method(Promoter Outside)
Group 1C：Landing pad (LP) construction and Insertion: Inducement and Recombination
Group 2A：Donor Plasmid Construction: Two Fragments Construction (Three Fragments Ligation)
Group 2B：Donor Plasmid Construction: Traditional Four Fragments Construction
Group 2C：Donor Plasmid Construction: Four Fragments Construction with Dra III
Group 2D：Donor Plasmid Construction: Four Fragments Construction with Novel Methods
Group 3：Helper Plasmid (HP) Insertion, Donor Plasmid Insertion, Recombination Induction & Removal of HP
Group 4：Screening Strategy
</div></div>
<a name="prt"></a>
Protocols
Here are our Experimental Protocols in PDF version. You're advised to view in <a href="http://www.adobe.com/acom/" target=blank>Acrobat</a> or <a href="http://get.adobe.com/reader/" target=blank>Adobe Reader</a> which you can download free from the<a href="http://www.adobe.com" target=blank> Home Website of Adobe® Company</a>, for other viewers we can not guarantee the right format of viewing. Those files are encrypted to keep copyrights, if you want to print them out of copy to your documents, to share with others our ideas and methods, you are welcome to ask us for the unencrypted files definitely for free~
Molecular Cloning
<a href="https://static.igem.org/mediawiki/2010/0/0a/THUProtocol_1-1_Isolatio_of_plasmid_DNA.pdf" target=blank>THUProtocol_1-1_Isolatio_of_plasmid_DNA</a>
<a href="https://static.igem.org/mediawiki/2010/d/d8/THUProtocol_1-2_DNA_Gel_Extraction.pdf" target=blank>THUProtocol_1-2_DNA_Gel_Extraction</a>
<a href="https://static.igem.org/mediawiki/2010/5/59/THUProtocol_1-3_Restriction_Enzyme_Digestion.pdf" target=blank>THUProtocol_1-3_Restriction_Enzyme_Digestion</a>
<a href="https://static.igem.org/mediawiki/2010/b/b7/THUProtocol_1-4_Ligation_of_DNA_Fragments.pdf" target=blank>THUProtocol_1-4_Ligation_of_DNA_Fragments</a>
<a href="https://static.igem.org/mediawiki/2010/c/c3/THUProtocol_1-5_PCR.pdf" target=blank>THUProtocol_1-5_PCR</a>
<a href="https://static.igem.org/mediawiki/2010/c/c0/THUProtocol_1-6_Preparation_of_Competent_Cell_for_Electro_Transformation.pdf" target=blank>THUProtocol_1-6_Preparation_of_Competent_Cell_for_Electro_Transformation</a>
<a href="https://static.igem.org/mediawiki/2010/7/77/THUProtocol_1-7_Chemical_Transformation_of_Recombinant_DNA.pdf" target=blank>THUProtocol_1-7_Chemical_Transformation_of_Recombinant_DNA</a>
<a href="https://static.igem.org/mediawiki/2010/4/40/THUProtocol_1-8_Electro_Transformation_of_Recombinant_DNA.pdf" target=blank>THUProtocol_1-8_Electro_Transformation_of_Recombinant_DNA</a>
Protein Isolation and Identification
<a href="https://static.igem.org/mediawiki/2010/f/f0/THUProtocol_2-1_Protein_Isolation_for_Prokaryotes.pdf" target=blank>THUProtocol_2-1_Protein_Isolation_for_Prokaryotes</a>
<a href="https://static.igem.org/mediawiki/2010/0/08/THUProtocol_2-2_Protein_Identification_SDS-PAGE.pdf" target=blank>THUProtocol_2-2_Protein_Identification_SDS-PAGE</a>
<a href="https://static.igem.org/mediawiki/2010/c/c3/THUProtocol_1-5_PCR.pdf" target=blank>THUProtocol_1-5_PCR</a>
</div> </div></div> </body> </html>
