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Team:Tsinghua/Notebook/3 August 2010
From 2010.igem.org
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== Module I, DT and Fan's part: == | == Module I, DT and Fan's part: == | ||
Amplify the plasmids pBMN-PIB, pKD13 and pKD3 with competent cells trans5α. All use 0.1% Amp to select. | Amplify the plasmids pBMN-PIB, pKD13 and pKD3 with competent cells trans5α. All use 0.1% Amp to select. | ||
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== Module I, YX and ZY's part: == | == Module I, YX and ZY's part: == | ||
We digested former PCR products with DraIII for over 17 hours. After agarose electrophoresis, we extracted pure products from the gel and ligated with Takara Ligation Solution I. | We digested former PCR products with DraIII for over 17 hours. After agarose electrophoresis, we extracted pure products from the gel and ligated with Takara Ligation Solution I. | ||
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| + | Besides, colonies with T vector grew on the plates. We picked several colonies and cultured them in 3ml LB media in the shaker. | ||
Revision as of 13:51, 7 September 2010
Module I, DT and Fan's part:
Amplify the plasmids pBMN-PIB, pKD13 and pKD3 with competent cells trans5α. All use 0.1% Amp to select.
Module I, YX and ZY's part:
We digested former PCR products with DraIII for over 17 hours. After agarose electrophoresis, we extracted pure products from the gel and ligated with Takara Ligation Solution I.
Besides, colonies with T vector grew on the plates. We picked several colonies and cultured them in 3ml LB media in the shaker.
