"
Team:Tsinghua/Notebook/30 July 2010
From 2010.igem.org
(Difference between revisions)
(New page: == Module I, Group 2c == To get target fragments with higher concentration, PCR and gel purification is conducted. PCR system and program is the same as what I used yesterday. ---- Resul...) |
(→Module I, Group 2c) |
||
| Line 3: | Line 3: | ||
PCR system and program is the same as what I used yesterday. | PCR system and program is the same as what I used yesterday. | ||
---- | ---- | ||
| - | Result: to my disappoint, after gel purification,target fragments disappeared! The concentration of fragments is less than 10ng/ul, which is far from enough for following digestion and ligation. Besides, | + | Result: to my disappoint, after gel purification,target fragments disappeared! The concentration of fragments is less than 10ng/ul, which is far from enough for following digestion and ligation. Besides, dimerization of primers is severe. |
Latest revision as of 07:36, 25 October 2010
Module I, Group 2c
To get target fragments with higher concentration, PCR and gel purification is conducted. PCR system and program is the same as what I used yesterday.
Result: to my disappoint, after gel purification,target fragments disappeared! The concentration of fragments is less than 10ng/ul, which is far from enough for following digestion and ligation. Besides, dimerization of primers is severe.
