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Team:Tsinghua/Notebook/1 August 2010
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Windwingstar (Talk | contribs) (New page: Module One 1: PCR for Tet, Landing Pad added. Pyrobest Enzyme) |
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| - | Module One 1: | + | |
| + | == Module One 1: == | ||
PCR for Tet, Landing Pad added. | PCR for Tet, Landing Pad added. | ||
Pyrobest Enzyme | Pyrobest Enzyme | ||
| + | |||
| + | |||
| + | == Module I, Group 2c == | ||
| + | We are informed that the volume of PCR system somehow has an effect on the result. Usually, 25ul or 20ul is a better option than 50ul system. To further modify the condition for PCR, we compared the result of 25ul and 50ul system. | ||
| + | ---- | ||
| + | |||
| + | |||
| + | PCR system (pfu): | ||
| + | H2O 13ul | ||
| + | 5×buffer 5ul | ||
| + | dNTP 2.5μl | ||
| + | primer1 1μl | ||
| + | primer2 0.5μl | ||
| + | template 1μl | ||
| + | FastPFU 2μl | ||
| + | Total 25μl | ||
| + | |||
| + | ---- | ||
| + | PCR system (pfu): | ||
| + | H2O 26ul | ||
| + | 5×buffer 10ul | ||
| + | dNTP 5μl | ||
| + | primer1 2μl | ||
| + | primer2 1μl | ||
| + | template 2μl | ||
| + | FastPFU 4μl | ||
| + | Total 50μl | ||
| + | |||
| + | ---- | ||
| + | Result: No specific target bands are seen in the gel photograph. | ||
Latest revision as of 16:57, 26 October 2010
Module One 1:
PCR for Tet, Landing Pad added. Pyrobest Enzyme
Module I, Group 2c
We are informed that the volume of PCR system somehow has an effect on the result. Usually, 25ul or 20ul is a better option than 50ul system. To further modify the condition for PCR, we compared the result of 25ul and 50ul system.
PCR system (pfu):
H2O 13ul 5×buffer 5ul dNTP 2.5μl primer1 1μl primer2 0.5μl template 1μl FastPFU 2μl Total 25μl
PCR system (pfu):
H2O 26ul 5×buffer 10ul dNTP 5μl primer1 2μl primer2 1μl template 2μl FastPFU 4μl Total 50μl
Result: No specific target bands are seen in the gel photograph.
