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Team:Tsinghua/Notebook/14 July 2010
From 2010.igem.org
(Difference between revisions)
(New page: == Module I, DT and Fan's part: == Measure the concentration of the PCR products. Gene Concentration(μg/ml) OD 260/280 eGFP 95.9 1.73 mCherry 61.2 1...) |
(→Module I, DT and Fan's part:) |
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| Line 4: | Line 4: | ||
Gene Concentration(μg/ml) OD 260/280 | Gene Concentration(μg/ml) OD 260/280 | ||
| - | eGFP | + | eGFP 95.9 1.73 |
| - | mCherry | + | mCherry 61.2 1.74 |
| - | Kan | + | Kan 160.5 1.67 |
| - | Chlr | + | Chlr 167.5 1.74 |
Digest the four fragments with follow system at 37℃ for 10 hours. | Digest the four fragments with follow system at 37℃ for 10 hours. | ||
| Line 13: | Line 13: | ||
Digestion system: | Digestion system: | ||
| - | eGFP: H2O | + | eGFP: |
| - | + | H2O 23.5μl | |
| - | + | 10×buffer 5μl | |
| - | + | eGFP 20μl | |
| - | + | HindIII 1.5μl | |
| + | Total 50μl | ||
| - | mCherry: H2O | + | mCherry: |
| - | + | H2O 11.5μl | |
| - | + | 10×buffer 5μl | |
| - | + | mCherry 32μl | |
| - | + | HindIII 1.5μl | |
| + | Total 50μl | ||
| - | Kan: H2O | + | Kan: |
| - | + | H2O 31μl | |
| - | + | 10×buffer 5μl | |
| - | + | Kan 12.5μl | |
| - | + | KpnI 1.5μl | |
| + | Total 50μl | ||
| + | |||
| + | |||
| + | Chlr: | ||
| + | H2O 31.5μl | ||
| + | 10×buffer 5μl | ||
| + | Chlr 12μl | ||
| + | KpnI 1.5μl | ||
| + | Total 50μl | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
Productions purify and measure the concentration. | Productions purify and measure the concentration. | ||
Gene Concentration(μg/ml) OD 260/280 | Gene Concentration(μg/ml) OD 260/280 | ||
| - | eGFP | + | eGFP 38.4 1.66 |
| - | mCherry | + | mCherry 28.8 1.86 |
| - | Kan | + | Kan 43.7 2.11 |
| - | Chlr | + | Chlr 31.9 1.77 |
Revision as of 09:09, 3 September 2010
Module I, DT and Fan's part:
Measure the concentration of the PCR products.
Gene Concentration(μg/ml) OD 260/280 eGFP 95.9 1.73 mCherry 61.2 1.74 Kan 160.5 1.67 Chlr 167.5 1.74
Digest the four fragments with follow system at 37℃ for 10 hours.
Digestion system:
eGFP:
H2O 23.5μl 10×buffer 5μl eGFP 20μl HindIII 1.5μl Total 50μl
mCherry:
H2O 11.5μl 10×buffer 5μl mCherry 32μl HindIII 1.5μl Total 50μl
Kan:
H2O 31μl 10×buffer 5μl Kan 12.5μl KpnI 1.5μl Total 50μl
Chlr:
H2O 31.5μl 10×buffer 5μl Chlr 12μl KpnI 1.5μl Total 50μl
Productions purify and measure the concentration.
Gene Concentration(μg/ml) OD 260/280 eGFP 38.4 1.66 mCherry 28.8 1.86 Kan 43.7 2.11 Chlr 31.9 1.77
