"
Team:Tsinghua/Notebook/14 July 2010
From 2010.igem.org
(Difference between revisions)
(New page: == Module I, DT and Fan's part: == Measure the concentration of the PCR products. Gene Concentration(μg/ml) OD 260/280 eGFP 95.9 1.73 mCherry 61.2 1...) |
(→Module I, DT and Fan's part:) |
||
| (5 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | == Module I, | + | == Module I, group 2(b)== |
Measure the concentration of the PCR products. | Measure the concentration of the PCR products. | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
Digest the four fragments with follow system at 37℃ for 10 hours. | Digest the four fragments with follow system at 37℃ for 10 hours. | ||
| Line 13: | Line 7: | ||
Digestion system: | Digestion system: | ||
| - | eGFP: H2O 23.5μl | + | eGFP: |
| - | + | H2O 23.5μl | |
| - | + | 10×buffer 5μl | |
| - | + | eGFP 20μl | |
| - | + | HindIII 1.5μl | |
| + | Total 50μl | ||
| + | |||
| + | mCherry: | ||
| + | H2O 11.5μl | ||
| + | 10×buffer 5μl | ||
| + | mCherry 32μl | ||
| + | HindIII 1.5μl | ||
| + | Total 50μl | ||
| + | |||
| + | Kan: | ||
| + | H2O 31μl | ||
| + | 10×buffer 5μl | ||
| + | Kan 12.5μl | ||
| + | KpnI 1.5μl | ||
| + | Total 50μl | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | + | Chlr: | |
| - | + | H2O 31.5μl | |
| - | + | 10×buffer 5μl | |
| - | + | Chlr 12μl | |
| - | + | KpnI 1.5μl | |
| + | Total 50μl | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
Productions purify and measure the concentration. | Productions purify and measure the concentration. | ||
| - | Gene Concentration(μg/ml) OD 260/280 | + | Gene Concentration(μg/ml) OD 260/280 |
| - | + | eGFP 38.4 1.66 | |
| - | + | mCherry 28.8 1.86 | |
| - | + | Kan 43.7 2.11 | |
| - | + | Chlr 31.9 1.77 | |
Latest revision as of 06:50, 25 October 2010
Module I, group 2(b)
Measure the concentration of the PCR products.
Digest the four fragments with follow system at 37℃ for 10 hours.
Digestion system:
eGFP:
H2O 23.5μl 10×buffer 5μl eGFP 20μl HindIII 1.5μl Total 50μl
mCherry:
H2O 11.5μl 10×buffer 5μl mCherry 32μl HindIII 1.5μl Total 50μl
Kan:
H2O 31μl 10×buffer 5μl Kan 12.5μl KpnI 1.5μl Total 50μl
Chlr:
H2O 31.5μl 10×buffer 5μl Chlr 12μl KpnI 1.5μl Total 50μl
Productions purify and measure the concentration.
Gene Concentration(μg/ml) OD 260/280 eGFP 38.4 1.66 mCherry 28.8 1.86 Kan 43.7 2.11 Chlr 31.9 1.77
