Tsinghua University

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English

Our Project

Tsinghua iGEM 2010 Project is focused on developing a new Antibody Production Method using Synthetic Biology. The Aim is to Simulate Antibody Selection and Production Technology with Bacteria.

Traditional method of antibody production such as Hybridoma Technology is expensive and time consuming. In Hybridoma Technology, generation of B-Cell library is accomplished inside experimental animal, mouse for example; using Flow Cytometry to select a specific kind of antibody; then hybridoma would be prepared to harvest antibody.

In this project, we expect to achieve all these processes cheap and efficiently in E.coli Cell. The whole project can be divided into two modules:

MODULE I: Generation of Antibody Library

MODULE II: Selection of Specific Antibody

中文版

2010年清华大学iGEM项目的重点是开发新的生产方法使用抗体合成生物学。其目的是模拟抗体的选择和生产工艺细菌。 传统方法生产的抗体杂交瘤细胞等技术是昂贵和费时。在杂交瘤技术，对B -细胞库的一代内完成实验动物，比如老鼠;使用流式细胞仪来选择一个特定类型的抗体，然后将准备杂交收获抗体。 在这个项目中，我们期待实现所有这些进程在大肠杆菌中高效廉价细胞。整个项目可分为两个模块：