Tsinghua University

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Our Project

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Tsinghua iGEM 2010 Project is focused on developing a new Antibody Production Method using Synthetic Biology. The Aim is to Simulate Antibody Selection and Production Technology with Bacteria.

Traditional method of antibody production such as Hybridoma Technology is expensive and time consuming. In Hybridoma Technology, generation of B-Cell library is accomplished inside experimental animal, mouse for example; using Flow Cytometry to select a specific kind of antibody; then hybridoma would be prepared to harvest antibody.

In this project, we expect to achieve all these processes cheap and efficiently in E.coli Cell. The whole project can be divided into two modules:

MODULE I: Generation of Antibody Library

We pre-modified the genome of E. coli by inserting a sequence with the I-Sel restriction sites. Then, into the plasmid of E. coli we inserted in both ends with two fragments which contain the recombinant sites (antibody variable region V, D, J). After being induced, recombination can be achieved by the crossed sequence. Specifically, as to we experiment, we will use fluorescent protein and resistance genes to testify.

MODULE II: Selection of Specific Antibody

Experimental design includes use of antigen-antibody binding, filtering through the filter called the "filtering and screening" and the application of the property as increased strength of cell adhesion caused by antigen-antibody binding to design the method called the "oscillation screening". The former is equivalent to using filters which is the most common and simple way of separating to achieve the purpose of separate and screen antibodies, while the latter one achieves the using of antibiotic resistance, which is the simplest and most convenient method in microbial operations, to separate and screen antibodies.

See Details @ Project

我们的项目

中文版Back

2010年清华大学iGEM项目的重点是开发新的生产方法使用抗体合成生物学。其目的是模拟抗体的选择和生产工艺细菌。 传统方法生产的抗体，比如杂交瘤细胞等技术，是昂贵和费时。杂交瘤技术，对B-细胞库构建是通过实验动物，比如老鼠;使用流式细胞仪来选择一个特异性抗体，然后用杂交瘤细胞收获抗体。 在这个项目中，我们期待在大肠杆菌细胞中用一种廉价高效的方法实现所有这些过程。整个项目可分为两个模块：

模块 I: 抗体库的构建

我们对大肠杆菌的基因组先进行了预修饰，对其插入了重组序列与I-Sel的酶切位点。然后，我们在质粒里插入两端有重组位点的目的片段（抗体可变区V,D,J），导入大肠杆菌，诱导后利用重组序列的交叉序列即可以重组。我们具体实验验证时，会利用荧光蛋白与抗性基因的筛选。

模块 II: 特异性抗体的筛选

实验设计主要包括利用抗原抗体结合，通过过滤方法进行筛选的“滤纸过滤筛选法”和应用抗原抗体结合后菌体间结合强度增加的性质设计的“振荡筛选法”。前者相当于利用过滤这种最常用和简便的分离方法达到了抗体分离的目的，而后者实现了用抗生素抗性这种微生物操作中最简便的筛选方法分离抗体的目的。

See Details @ Project

О проекте

РусскийBack

Tsinghua iGEM 2010 Project is focused on developing a new Antibody Production Method using Synthetic Biology. The Aim is to Simulate Antibody Selection and Production Technology with Bacteria.

Traditional method of antibody production such as Hybridoma Technology is expensive and time consuming. In Hybridoma Technology, generation of B-Cell library is accomplished inside experimental animal, mouse for example; using Flow Cytometry to select a specific kind of antibody; then hybridoma would be prepared to harvest antibody.

In this project, we expect to achieve all these processes cheap and efficiently in E.coli Cell. The whole project can be divided into two modules:

MODULE I: Generation of Antibody Library

MODULE II: Selection of Specific Antibody

See Details @ Project

Notre Projet

FrançaisBack

Tsinghua iGEM 2010 Project is focused on developing a new Antibody Production Method using Synthetic Biology. The Aim is to Simulate Antibody Selection and Production Technology with Bacteria.

Traditional method of antibody production such as Hybridoma Technology is expensive and time consuming. In Hybridoma Technology, generation of B-Cell library is accomplished inside experimental animal, mouse for example; using Flow Cytometry to select a specific kind of antibody; then hybridoma would be prepared to harvest antibody.

In this project, we expect to achieve all these processes cheap and efficiently in E.coli Cell. The whole project can be divided into two modules:

MODULE I: Generation of Antibody Library

MODULE II: Selection of Specific Antibody

See Details @ Project

Unser Projekt

DeutschBack

Tsinghua iGEM 2010 Project is focused on developing a new Antibody Production Method using Synthetic Biology. The Aim is to Simulate Antibody Selection and Production Technology with Bacteria.

Traditional method of antibody production such as Hybridoma Technology is expensive and time consuming. In Hybridoma Technology, generation of B-Cell library is accomplished inside experimental animal, mouse for example; using Flow Cytometry to select a specific kind of antibody; then hybridoma would be prepared to harvest antibody.

In this project, we expect to achieve all these processes cheap and efficiently in E.coli Cell. The whole project can be divided into two modules:

MODULE I: Generation of Antibody Library

MODULE II: Selection of Specific Antibody

See Details @ Project

Nuestro Proyecto

Español Back

Tsinghua iGEM 2010 Project is focused on developing a new Antibody Production Method using Synthetic Biology. The Aim is to Simulate Antibody Selection and Production Technology with Bacteria.

Traditional method of antibody production such as Hybridoma Technology is expensive and time consuming. In Hybridoma Technology, generation of B-Cell library is accomplished inside experimental animal, mouse for example; using Flow Cytometry to select a specific kind of antibody; then hybridoma would be prepared to harvest antibody.

In this project, we expect to achieve all these processes cheap and efficiently in E.coli Cell. The whole project can be divided into two modules:

MODULE I: Generation of Antibody Library

MODULE II: Selection of Specific Antibody

私たちのプロジェクト

日本語 Back

清華せいさつ2010プロジェクトは、新しい抗体の生産方法は、合成生物学を使用して開発に焦点を当てています。 目的には をシミュレート抗体の選択と生産技術細菌です。

ハイブリドーマ技術などの抗体産生の伝統的な方法は、コストと時間がかかります。ハイブリドーマ技術では、B細胞ライブラリの生成は実験動物内で、たとえばマウスが、抗体の特定の種類を選択するフローサイトメトリーを使用して、次にハイブリドーマは、収穫抗体を準備される実行されます。

このプロジェクトでは、我々は安く、大腸菌細胞を効率的に処理するすべてのこれらを実現する予定です。プロジェクト全体は、2つのモジュールに分割することができます：

モジュール I: 建設ののライブラリ

モジュール II: フィルタの特定の抗体