http://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/System&feed=atom&action=historyTeam:Tokyo Tech/Project/wolf coli/System - Revision history2024-03-29T10:47:25ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/System&diff=208494&oldid=prevODDY at 03:58, 28 October 20102010-10-28T03:58:46Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At low concentration of AHL(Fig. 4-3-6b), Lac IM1 and cI are expressed only at basal levels. This enables the expression of a LacIWT, again resulting in GFP repression.<br> <br> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At low concentration of AHL(Fig. 4-3-6b), Lac IM1 and cI are expressed only at basal levels. This enables the expression of a LacIWT, again resulting in GFP repression.<br> <br> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At intermediate concentration of AHL (Fig. 4-3-6c), this results in moderate levels of cI and LacIM1. However, because the repression efficiency of cI is significantly higher than that of LacIM1, cI effectively shuts off LacI expression while the LacIM1 concentration is below the threshold required to repress GFP production. This difference between the cI and LacIM1 repression efficiencies, in combination with a feed-forward loop that begins with LuxR and culminates in GFP, affords the circuit the desired non-monotonic response to AHL dosages.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At intermediate concentration of AHL (Fig. 4-3-6c), this results in moderate levels of cI and LacIM1. However, because the repression efficiency of cI is significantly higher than that of LacIM1, cI effectively shuts off LacI expression while the LacIM1 concentration is below the threshold required to repress GFP production. This difference between the cI and LacIM1 repression efficiencies, in combination with a feed-forward loop that begins with LuxR and culminates in GFP, affords the circuit the desired non-monotonic response to AHL dosages.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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</table>ODDYhttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/System&diff=208108&oldid=prevVancouver: /* Reference */2010-10-28T03:49:56Z<p><span class="autocomment">Reference</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At low concentration of AHL(Fig. 4-3-6b), Lac IM1 and cI are expressed only at basal levels. This enables the expression of a LacIWT, again resulting in GFP repression.<br> <br> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At low concentration of AHL(Fig. 4-3-6b), Lac IM1 and cI are expressed only at basal levels. This enables the expression of a LacIWT, again resulting in GFP repression.<br> <br> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At intermediate concentration of AHL (Fig. 4-3-6c), this results in moderate levels of cI and LacIM1. However, because the repression efficiency of cI is significantly higher than that of LacIM1, cI effectively shuts off LacI expression while the LacIM1 concentration is below the threshold required to repress GFP production. This difference between the cI and LacIM1 repression efficiencies, in combination with a feed-forward loop that begins with LuxR and culminates in GFP, affords the circuit the desired non-monotonic response to AHL dosages.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At intermediate concentration of AHL (Fig. 4-3-6c), this results in moderate levels of cI and LacIM1. However, because the repression efficiency of cI is significantly higher than that of LacIM1, cI effectively shuts off LacI expression while the LacIM1 concentration is below the threshold required to repress GFP production. This difference between the cI and LacIM1 repression efficiencies, in combination with a feed-forward loop that begins with LuxR and culminates in GFP, affords the circuit the desired non-monotonic response to AHL dosages.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Reference==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Reference==</div></td></tr>
</table>Vancouverhttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/System&diff=208107&oldid=prevXiang: /* Genetic circuit */2010-10-28T03:49:55Z<p><span class="autocomment">Genetic circuit</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''At daytime (Strong light intensity)<del class="diffchange diffchange-inline">''' </del>(Fig. 4-3-3)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''At daytime (Strong light intensity) (Fig. 4-3-3)<ins class="diffchange diffchange-inline">'''<br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. There is no expression from ''OmpC'' promoter because unphosphorylated OmpR doesn’t bind to ''OmpC'' promoter. In low ratio of phosphorylated OmpR, LacIM1 and cI are expressed only at basal levels. This enables the expression of a wild-type LacI, again resulting in repression of the inverter. Thus again Artificial Cooperation System turns on, and two types of cell would save each other from dying while communicating with each other. [[Image: tokyotech_wolfcoli_daytime.png|center|thumb|400px|Figure 4-3-3.Wolfcoli system at daytime]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. There is no expression from ''OmpC'' promoter because unphosphorylated OmpR doesn’t bind to ''OmpC'' promoter. In low ratio of phosphorylated OmpR, LacIM1 and cI are expressed only at basal levels. This enables the expression of a wild-type LacI, again resulting in repression of the inverter. Thus again Artificial Cooperation System turns on, and two types of cell would save each other from dying while communicating with each other. [[Image: tokyotech_wolfcoli_daytime.png|center|thumb|400px|Figure 4-3-3.Wolfcoli system at daytime]]</div></td></tr>
</table>Xianghttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/System&diff=208081&oldid=prevVancouver: /* Band-detect network */2010-10-28T03:49:08Z<p><span class="autocomment">Band-detect network</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Previously, USTC(2008) attempted to build this circuit and registered parts for this circuit[3].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Previously, USTC(2008) attempted to build this circuit and registered parts for this circuit[3].</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">In </del>Basu’s group paper, LuxR, an AHL-dependent transcriptional regulator was used.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">According to </ins>Basu’s group paper, LuxR, an AHL-dependent transcriptional regulator was used.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>LuxR activates the expression of cI and LacIM1. LacIM1 shows weaker repression than LacIWT because it has a lower affinity to lac promoter (Fig. 4-3-5).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>LuxR activates the expression of cI and LacIM1. LacIM1 shows weaker repression than LacIWT because it has a lower affinity to lac promoter (Fig. 4-3-5).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At low concentration of AHL(Fig. 4-3-6b), Lac IM1 and cI are expressed only at basal levels. This enables the expression of a LacIWT, again resulting in GFP repression.<br> <br> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At low concentration of AHL(Fig. 4-3-6b), Lac IM1 and cI are expressed only at basal levels. This enables the expression of a LacIWT, again resulting in GFP repression.<br> <br> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At intermediate concentration of AHL (Fig. 4-3-6c), this results in moderate levels of cI and LacIM1. However, because the repression efficiency of cI is significantly higher than that of LacIM1, cI effectively shuts off LacI expression while the LacIM1 concentration is below the threshold required to repress GFP production. This difference between the cI and LacIM1 repression efficiencies, in combination with a feed-forward loop that begins with LuxR and culminates in GFP, affords the circuit the desired non-monotonic response to AHL dosages.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At intermediate concentration of AHL (Fig. 4-3-6c), this results in moderate levels of cI and LacIM1. However, because the repression efficiency of cI is significantly higher than that of LacIM1, cI effectively shuts off LacI expression while the LacIM1 concentration is below the threshold required to repress GFP production. This difference between the cI and LacIM1 repression efficiencies, in combination with a feed-forward loop that begins with LuxR and culminates in GFP, affords the circuit the desired non-monotonic response to AHL dosages.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Reference==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Reference==</div></td></tr>
</table>Vancouverhttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/System&diff=208047&oldid=prevXiang at 03:48, 28 October 20102010-10-28T03:48:01Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">・During </del>dark nights (Weak light intensity) (Fig. 4-3-2)<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''During </ins>dark nights (Weak light intensity) (Fig. 4-3-2)<ins class="diffchange diffchange-inline">'''</ins><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. Since unphosphorylated OmpR doesn’t bind to ''OmpC'' promoter, there would be only basal expression level of ''OmpC'' promoter. In this condition LacIM1 and cI are expressed at basal levels. This enables the expression of a wild-type LacI and again results in repression of the inverter. Thus again Artificial Cooperation System turns on, and two types of cell would save each other from dying while communicating with each other. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. Since unphosphorylated OmpR doesn’t bind to ''OmpC'' promoter, there would be only basal expression level of ''OmpC'' promoter. In this condition LacIM1 and cI are expressed at basal levels. This enables the expression of a wild-type LacI and again results in repression of the inverter. Thus again Artificial Cooperation System turns on, and two types of cell would save each other from dying while communicating with each other. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> [[Image: tokyotech_wolfcoli_night.png|center|thumb|400px|Figure 4-3-2.Wolfcoli system at crescent moon night]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> [[Image: tokyotech_wolfcoli_night.png|center|thumb|400px|Figure 4-3-2.Wolfcoli system at crescent moon night]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">・At </del>daytime (Strong light intensity) (Fig. 4-3-3)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''At </ins>daytime (Strong light intensity)<ins class="diffchange diffchange-inline">''' </ins>(Fig. 4-3-3)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. There is no expression from ''OmpC'' promoter because unphosphorylated OmpR doesn’t bind to ''OmpC'' promoter. In low ratio of phosphorylated OmpR, LacIM1 and cI are expressed only at basal levels. This enables the expression of a wild-type LacI, again resulting in repression of the inverter. Thus again Artificial Cooperation System turns on, and two types of cell would save each other from dying while communicating with each other. [[Image: tokyotech_wolfcoli_daytime.png|center|thumb|400px|Figure 4-3-3.Wolfcoli system at daytime]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. There is no expression from ''OmpC'' promoter because unphosphorylated OmpR doesn’t bind to ''OmpC'' promoter. In low ratio of phosphorylated OmpR, LacIM1 and cI are expressed only at basal levels. This enables the expression of a wild-type LacI, again resulting in repression of the inverter. Thus again Artificial Cooperation System turns on, and two types of cell would save each other from dying while communicating with each other. [[Image: tokyotech_wolfcoli_daytime.png|center|thumb|400px|Figure 4-3-3.Wolfcoli system at daytime]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">・At </del>full moon night (Medium light intensity) (Fig. 4-3-4)<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''At </ins>full moon night (Medium light intensity) (Fig. 4-3-4)<ins class="diffchange diffchange-inline">'''</ins><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Medium level of phosphorylated OmpR ratio results in moderate levels of cI and LacIM1. However, because the repression efficiency of cI is significantly higher than that of LacIM1, cI effectively shuts off LacI expression while the LacIM1 concentration is below the threshold required to repress the inverter. This difference between the cI and LacIM1 repression efficiencies drives the inverter to a state of turning on. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Medium level of phosphorylated OmpR ratio results in moderate levels of cI and LacIM1. However, because the repression efficiency of cI is significantly higher than that of LacIM1, cI effectively shuts off LacI expression while the LacIM1 concentration is below the threshold required to repress the inverter. This difference between the cI and LacIM1 repression efficiencies drives the inverter to a state of turning on. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This results in turning off the Artificial Cooperation System. Thus, the two types of cell would not rescue each other in crisis situation since they can’t communicate. Due to these behaviors, we called the designed E.coli“Wolfcoli”.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This results in turning off the Artificial Cooperation System. Thus, the two types of cell would not rescue each other in crisis situation since they can’t communicate. Due to these behaviors, we called the designed E.coli“Wolfcoli”.</div></td></tr>
</table>Xianghttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/System&diff=207911&oldid=prevVancouver: /* Red-light-dependent gene expression network */2010-10-28T03:42:40Z<p><span class="autocomment">Red-light-dependent gene expression network</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Photoreceptors are not found in E. coli. Then, they introduced a light sensor from a cyanobacterium into E. coli. The response regulator of phytochrome does not directly regulate gene expression, so they fused a cyanobacterial photoreceptor from Cph1 to an E. coli intracellular histidine kinase domain and response-regulator from EnvZ–OmpR. Moreover, Cph1–EnvZ chimaeras were then activated by introduction of two phycocyanobilin-biosynthesis genes that convert heme into phycocyanobilin.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Photoreceptors are not found in E. coli. Then, they introduced a light sensor from a cyanobacterium into E. coli. The response regulator of phytochrome does not directly regulate gene expression, so they fused a cyanobacterial photoreceptor from Cph1 to an E. coli intracellular histidine kinase domain and response-regulator from EnvZ–OmpR. Moreover, Cph1–EnvZ chimaeras were then activated by introduction of two phycocyanobilin-biosynthesis genes that convert heme into phycocyanobilin.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In weak red light condition, Cph1–EnvZ chimeras are activated. EnvZ is autophosphorylated and passes phosphoryl group intramolecularly to OmpR. Then, phosphorylated OmpR binds to <del class="diffchange diffchange-inline">ompC </del>promoter and activates the transcription of the downstream gene.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In weak red light condition, Cph1–EnvZ chimeras are activated. EnvZ is autophosphorylated and passes phosphoryl group intramolecularly to OmpR. Then, phosphorylated OmpR binds to <ins class="diffchange diffchange-inline">''OmpC'' </ins>promoter and activates the transcription of the downstream gene.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In strong red light condition, Cph1–EnvZ chimaeras are not activated. EnvZ is dephosphorylated, and thus phosphorylation of OmpR doesn’t occur. Then, OmpR can’t bind to <del class="diffchange diffchange-inline">ompC </del>promoter, Therefore, the transcription of the downstream gene doesn’t occur<del class="diffchange diffchange-inline">. </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In strong red light condition, Cph1–EnvZ chimaeras are not activated. EnvZ is dephosphorylated, and thus phosphorylation of OmpR doesn’t occur. Then, OmpR can’t bind to <ins class="diffchange diffchange-inline">''OmpC'' </ins>promoter, Therefore, the transcription of the downstream gene doesn’t occur. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">The important point in this system is, light intensity determines expression of the ratio of phosphorylated OmpR</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The important point in this system is, light intensity determines expression of the ratio of phosphorylated OmpR.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Band-detect network==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Band-detect network==</div></td></tr>
</table>Vancouverhttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/System&diff=207870&oldid=prevXiang at 03:40, 28 October 20102010-10-28T03:40:36Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Genetic circuit==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Genetic circuit==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*;</del>circuit</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''</ins>circuit<ins class="diffchange diffchange-inline">'''</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image: tokyotech_wolfcoli_system_ver5.png|center|thumb|500px|Figure 4-3-1.Wolfcoli system ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image: tokyotech_wolfcoli_system_ver5.png|center|thumb|500px|Figure 4-3-1.Wolfcoli system ]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We aimed to introduce a red-light-dependent gene expression network and the band-detect network into the Artificial Cooperation System. (Fig. 4-3-1)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We aimed to introduce a red-light-dependent gene expression network and the band-detect network into the Artificial Cooperation System. (Fig. 4-3-1)</div></td></tr>
</table>Xianghttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/System&diff=207823&oldid=prevVancouver: /* Genetic circuit */2010-10-28T03:38:01Z<p><span class="autocomment">Genetic circuit</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Genetic circuit==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Genetic circuit==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">・circuit</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*;circuit</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image: tokyotech_wolfcoli_system_ver5.png|center|thumb|500px|Figure 4-3-1.Wolfcoli system ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image: tokyotech_wolfcoli_system_ver5.png|center|thumb|500px|Figure 4-3-1.Wolfcoli system ]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We aimed to introduce a red-light-dependent gene expression network and the band-detect network into the Artificial Cooperation System. (Fig. 4-3-1)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We aimed to introduce a red-light-dependent gene expression network and the band-detect network into the Artificial Cooperation System. (Fig. 4-3-1)</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・During dark nights (Weak light intensity) (Fig. 4-3-2)<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・During dark nights (Weak light intensity) (Fig. 4-3-2)<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. Since unphosphorylated OmpR doesn’t bind to <del class="diffchange diffchange-inline">ompC </del>promoter, there would be only basal expression level of <del class="diffchange diffchange-inline">ompC </del>promoter. In this condition LacIM1 and cI are expressed at basal levels. This enables the expression of a wild-type LacI and again results in repression of the inverter. Thus again Artificial Cooperation System turns on, and two types of cell would save each other from dying while communicating with each other. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. Since unphosphorylated OmpR doesn’t bind to <ins class="diffchange diffchange-inline">''OmpC'' </ins>promoter, there would be only basal expression level of <ins class="diffchange diffchange-inline">''OmpC'' </ins>promoter. In this condition LacIM1 and cI are expressed at basal levels. This enables the expression of a wild-type LacI and again results in repression of the inverter. Thus again Artificial Cooperation System turns on, and two types of cell would save each other from dying while communicating with each other. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> [[Image: tokyotech_wolfcoli_night.png|center|thumb|400px|Figure 4-3-2.Wolfcoli system at crescent moon night]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> [[Image: tokyotech_wolfcoli_night.png|center|thumb|400px|Figure 4-3-2.Wolfcoli system at crescent moon night]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At daytime (Strong light intensity) (Fig. 4-3-3)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At daytime (Strong light intensity) (Fig. 4-3-3)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. There is no expression from <del class="diffchange diffchange-inline">ompC </del>promoter because unphosphorylated OmpR doesn’t bind to <del class="diffchange diffchange-inline">ompC </del>promoter. In low ratio of phosphorylated OmpR, LacIM1 and cI are expressed only at basal levels. This enables the expression of a wild-type LacI, again resulting in repression of the inverter. Thus again Artificial Cooperation System turns on, and two types of cell would save each other from dying while communicating with each other. [[Image: tokyotech_wolfcoli_daytime.png|center|thumb|400px|Figure 4-3-3.Wolfcoli system at daytime]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. There is no expression from <ins class="diffchange diffchange-inline">''OmpC'' </ins>promoter because unphosphorylated OmpR doesn’t bind to <ins class="diffchange diffchange-inline">''OmpC'' </ins>promoter. In low ratio of phosphorylated OmpR, LacIM1 and cI are expressed only at basal levels. This enables the expression of a wild-type LacI, again resulting in repression of the inverter. Thus again Artificial Cooperation System turns on, and two types of cell would save each other from dying while communicating with each other. [[Image: tokyotech_wolfcoli_daytime.png|center|thumb|400px|Figure 4-3-3.Wolfcoli system at daytime]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
</table>Vancouverhttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/System&diff=205466&oldid=prevODDY: /* Genetic circuit */2010-10-28T02:25:35Z<p><span class="autocomment">Genetic circuit</span></p>
<table style="background-color: white; color:black;">
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Genetic circuit==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Genetic circuit==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・circuit</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・circuit</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image: <del class="diffchange diffchange-inline">tokyotech_wolfcoli_system</del>.png|center|thumb|500px|Figure 4-3-1.Wolfcoli system ]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image: <ins class="diffchange diffchange-inline">tokyotech_wolfcoli_system_ver5</ins>.png|center|thumb|500px|Figure 4-3-1.Wolfcoli system ]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We aimed to introduce a red-light-dependent gene expression network and the band-detect network into the Artificial Cooperation System. (Fig. 4-3-1)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We aimed to introduce a red-light-dependent gene expression network and the band-detect network into the Artificial Cooperation System. (Fig. 4-3-1)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>These two systems were integrated and linked together by applying OmpR. OmpR usually works as a regulative factor of red-light sensing system, and we utilized this to regulate the band-detect network. OmpR is a transcriptional regulator, which activates the expression of cI and LacIM1 in this system</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>These two systems were integrated and linked together by applying OmpR. OmpR usually works as a regulative factor of red-light sensing system, and we utilized this to regulate the band-detect network. OmpR is a transcriptional regulator, which activates the expression of cI and LacIM1 in this system</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>・During dark nights (Weak light intensity) (Fig. 4-3-2)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>・During dark nights (Weak light intensity) (Fig. 4-3-2)<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. Since unphosphorylated OmpR doesn’t bind to ompC promoter, there would be only basal expression level of ompC promoter. In this condition LacIM1 and cI are expressed at basal levels. This enables the expression of a wild-type LacI and again results in repression of the inverter. Thus again Artificial Cooperation System turns on, and two types of cell would save each other from dying while communicating with each other. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. Since unphosphorylated OmpR doesn’t bind to ompC promoter, there would be only basal expression level of ompC promoter. In this condition LacIM1 and cI are expressed at basal levels. This enables the expression of a wild-type LacI and again results in repression of the inverter. Thus again Artificial Cooperation System turns on, and two types of cell would save each other from dying while communicating with each other. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> [[Image: tokyotech_wolfcoli_night.png|center|thumb|400px|Figure 4-3-2.Wolfcoli system at crescent moon night]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> [[Image: tokyotech_wolfcoli_night.png|center|thumb|400px|Figure 4-3-2.Wolfcoli system at crescent moon night]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At daytime (Strong light intensity) (Fig. 4-3-3)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At daytime (Strong light intensity) (Fig. 4-3-3)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">・At daytime (Strong light intensity) (Fig. 4-3-3) </del>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. There is no expression from ompC promoter because unphosphorylated OmpR doesn’t bind to ompC promoter. In low ratio of phosphorylated OmpR, LacIM1 and cI are expressed only at basal levels. This enables the expression of a wild-type LacI, again resulting in repression of the inverter. Thus again Artificial Cooperation System turns on, and two types of cell would save each other from dying while communicating with each other. [[Image: tokyotech_wolfcoli_daytime.png|center|thumb|400px|Figure 4-3-3.Wolfcoli system at daytime]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. There is no expression from ompC promoter because unphosphorylated OmpR doesn’t bind to ompC promoter. In low ratio of phosphorylated OmpR, LacIM1 and cI are expressed only at basal levels. This enables the expression of a wild-type LacI, again resulting in repression of the inverter. Thus again Artificial Cooperation System turns on, and two types of cell would save each other from dying while communicating with each other. [[Image: tokyotech_wolfcoli_daytime.png|center|thumb|400px|Figure 4-3-3.Wolfcoli system at daytime]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>・At full moon night (Medium light intensity) (Fig. 4-3-4)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>・At full moon night (Medium light intensity) (Fig. 4-3-4)<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Medium level of phosphorylated OmpR ratio results in moderate levels of cI and LacIM1. However, because the repression efficiency of cI is significantly higher than that of LacIM1, cI effectively shuts off LacI expression while the LacIM1 concentration is below the threshold required to repress the inverter. This difference between the cI and LacIM1 repression efficiencies drives the inverter to a state of turning on. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Medium level of phosphorylated OmpR ratio results in moderate levels of cI and LacIM1. However, because the repression efficiency of cI is significantly higher than that of LacIM1, cI effectively shuts off LacI expression while the LacIM1 concentration is below the threshold required to repress the inverter. This difference between the cI and LacIM1 repression efficiencies drives the inverter to a state of turning on. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This results in turning off the Artificial Cooperation System. Thus, the two types of cell would not rescue each other in crisis situation since they can’t communicate. Due to these behaviors, we called the designed E.coli“Wolfcoli”.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This results in turning off the Artificial Cooperation System. Thus, the two types of cell would not rescue each other in crisis situation since they can’t communicate. Due to these behaviors, we called the designed E.coli“Wolfcoli”.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> [[Image: <del class="diffchange diffchange-inline">tokyotech_wolfcoli_fullmoon night</del>.png|center|thumb|400px|Figure 4-3-4.Wolfcoli system at full moon night]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> [[Image: <ins class="diffchange diffchange-inline">tokyotech_wolfcoli_fullmoon_ver5</ins>.png|center|thumb|400px|Figure 4-3-4.Wolfcoli system at full moon night]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Red-light-dependent gene expression network==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Red-light-dependent gene expression network==</div></td></tr>
</table>ODDYhttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/System&diff=205240&oldid=prevODDY at 02:18, 28 October 20102010-10-28T02:18:08Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:18, 28 October 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image: tokyotech_wolfcoli_system.png|center|thumb|500px|Figure 4-3-1.Wolfcoli system ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image: tokyotech_wolfcoli_system.png|center|thumb|500px|Figure 4-3-1.Wolfcoli system ]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We aimed to introduce a red-light-dependent gene expression network and the band-detect network into the Artificial Cooperation System. (Fig. 4-3-1)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We aimed to introduce a red-light-dependent gene expression network and the band-detect network into the Artificial Cooperation System. (Fig. 4-3-1)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Two networks </del>were <del class="diffchange diffchange-inline">united </del>by applying OmpR. OmpR usually works as regulative factor of red-light sensing system, and we utilized this to regulate the band-detect network. OmpR is a transcriptional regulator, which activates the expression of <del class="diffchange diffchange-inline">lambda repressor (</del>cI<del class="diffchange diffchange-inline">) </del>and <del class="diffchange diffchange-inline">Lac repressor (</del>LacIM1<del class="diffchange diffchange-inline">, a product of a codon-modified lacI) </del>in <del class="diffchange diffchange-inline">Wolfcoli </del>system</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> These two systems </ins>were <ins class="diffchange diffchange-inline">integrated and linked together </ins>by applying OmpR. OmpR usually works as <ins class="diffchange diffchange-inline">a </ins>regulative factor of red-light sensing system, and we utilized this to regulate the band-detect network. OmpR is a transcriptional regulator, which activates the expression of cI and LacIM1 in <ins class="diffchange diffchange-inline">this </ins>system</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">・At night </del>(Weak light intensity) (Fig. 4-3-2)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">・During dark nights </ins>(Weak light intensity) (Fig. 4-3-2)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Light drives </ins>the <ins class="diffchange diffchange-inline">sensor to a state in which phosphorylation </ins>of OmpR <ins class="diffchange diffchange-inline">is inhibited. Since unphosphorylated OmpR doesn’t bind to ompC promoter, there would be only basal expression level of </ins>ompC promoter. <ins class="diffchange diffchange-inline"> In this condition LacIM1 and cI are expressed at basal </ins>levels<ins class="diffchange diffchange-inline">. This enables the expression </ins>of <ins class="diffchange diffchange-inline">a wild-type LacI </ins>and <ins class="diffchange diffchange-inline">again results in </ins>repression of the inverter. <ins class="diffchange diffchange-inline">Thus again </ins>Artificial Cooperation System turns on, and two types of cell <ins class="diffchange diffchange-inline">would save each other from dying while communicating with </ins>each other. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">In </del>the <del class="diffchange diffchange-inline">dark, high ratio </del>of <del class="diffchange diffchange-inline">phosphorylated </del>OmpR <del class="diffchange diffchange-inline">activates </del>ompC promoter. <del class="diffchange diffchange-inline">This results in high cytoplasmic </del>levels of <del class="diffchange diffchange-inline">cI and LacIM1 </del>and repression of the inverter. <del class="diffchange diffchange-inline"> Then, </del>Artificial Cooperation System turns on, and <del class="diffchange diffchange-inline">two cells are able to communicate. Then, </del>two types of cell <del class="diffchange diffchange-inline">help </del>each other. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>[[Image: tokyotech_wolfcoli_night.png|center|thumb|400px|Figure 4-3-2.Wolfcoli system at crescent moon night]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image: tokyotech_wolfcoli_night.png|center|thumb|400px|Figure 4-3-2.Wolfcoli system at crescent moon night]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At daytime (Strong light intensity) (Fig. 4-3-3)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At daytime (Strong light intensity) (Fig. 4-3-3)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. There is no expression from ompC promoter because unphosphorylated OmpR doesn’t bind to ompC promoter. <del class="diffchange diffchange-inline"> </del>In low ratio of phosphorylated OmpR, LacIM1 and cI are expressed only at basal levels. <del class="diffchange diffchange-inline"> </del>This enables the expression of a wild-type LacI, again resulting in repression of the inverter. <del class="diffchange diffchange-inline"> Then, </del>Artificial Cooperation System <del class="diffchange diffchange-inline">also </del>turns on, and <del class="diffchange diffchange-inline">two cells are able to communicate, too. Then, </del>two types of cell <del class="diffchange diffchange-inline">also help </del>each other. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image: tokyotech_wolfcoli_daytime.png|center|thumb|400px|Figure 4-3-3.Wolfcoli system at daytime]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">・At daytime (Strong light intensity) (Fig. 4-3-3) </ins>Light drives the sensor to a state in which phosphorylation of OmpR is inhibited. There is no expression from ompC promoter because unphosphorylated OmpR doesn’t bind to ompC promoter. In low ratio of phosphorylated OmpR, LacIM1 and cI are expressed only at basal levels. This enables the expression of a wild-type LacI, again resulting in repression of the inverter. <ins class="diffchange diffchange-inline">Thus again </ins>Artificial Cooperation System turns on, and two types of cell <ins class="diffchange diffchange-inline">would save each other from dying while communicating with </ins>each other. [[Image: tokyotech_wolfcoli_daytime.png|center|thumb|400px|Figure 4-3-3.Wolfcoli system at daytime]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At full moon night (Medium light intensity) (Fig. 4-3-4)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・At full moon night (Medium light intensity) (Fig. 4-3-4)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Medium level of phosphorylated OmpR ratio results in moderate levels of cI and LacIM1. <del class="diffchange diffchange-inline"> </del>However, because the repression efficiency of cI is significantly higher than that of LacIM1, cI effectively shuts off LacI expression while the LacIM1 concentration is below the threshold required to repress the inverter. <del class="diffchange diffchange-inline"> </del>This difference between the cI and LacIM1 repression efficiencies drives the inverter to a state of turning on. <del class="diffchange diffchange-inline"> </del>This results in turning off the Artificial Cooperation System, <del class="diffchange diffchange-inline">and </del>two <del class="diffchange diffchange-inline">cells </del>can’t communicate. <del class="diffchange diffchange-inline">two cells start competing with each other at full moon night . From </del>these <del class="diffchange diffchange-inline">behavior</del>, we <del class="diffchange diffchange-inline">call this “Wolfcoli”</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Medium level of phosphorylated OmpR ratio results in moderate levels of cI and LacIM1. However, because the repression efficiency of cI is significantly higher than that of LacIM1, cI effectively shuts off LacI expression while the LacIM1 concentration is below the threshold required to repress the inverter. This difference between the cI and LacIM1 repression efficiencies drives the inverter to a state of turning on. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image: tokyotech_wolfcoli_fullmoon night.png|center|thumb|400px|Figure 4-3-4.Wolfcoli system at full moon night]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This results in turning off the Artificial Cooperation System<ins class="diffchange diffchange-inline">. Thus</ins>, <ins class="diffchange diffchange-inline">the </ins>two <ins class="diffchange diffchange-inline">types of cell would not rescue each other in crisis situation since they </ins>can’t communicate. <ins class="diffchange diffchange-inline">Due to </ins>these <ins class="diffchange diffchange-inline">behaviors</ins>, we <ins class="diffchange diffchange-inline">called the designed E.coli“Wolfcoli”</ins>.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>[[Image: tokyotech_wolfcoli_fullmoon night.png|center|thumb|400px|Figure 4-3-4.Wolfcoli system at full moon night]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Red-light-dependent gene expression network==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Red-light-dependent gene expression network==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. Basu S et al. A synthetic multicellular system for programmed pattern formation. NATURE 2005;434,1130-1134 <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2. Basu S et al. A synthetic multicellular system for programmed pattern formation. NATURE 2005;434,1130-1134 <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3.[https://2008.igem.org/Team:USTC USTC(2008)]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3.[https://2008.igem.org/Team:USTC USTC(2008)]</div></td></tr>
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</table>ODDY