http://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC&feed=atom&action=historyTeam:Tokyo Tech/Project/wolf coli/New Series of PompC - Revision history2024-03-28T15:19:20ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC&diff=207697&oldid=prevVancouver: /* Result */2010-10-28T03:34:24Z<p><span class="autocomment">Result</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*; Characterization of new series of ''OmpC'' promoters</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*; Characterization of new series of ''OmpC'' promoters</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Tokyotech_ompc_graph.jpg|thumb|center|400px|thumb|Fig.4-1-1 The induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA ]] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Tokyotech_ompc_graph.jpg|thumb|center|400px|thumb|Fig.4-1-1 The induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA ]] </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>After 4 hours of sucrose induction, transcriptional activity of <del class="diffchange diffchange-inline">PompC</del>(CB)-GFP and <del class="diffchange diffchange-inline">PompC</del>(CS1)-GFP increased 2.5-folds and 2.3-folds respectively. However, significant amount of leaky expression was found in <del class="diffchange diffchange-inline">PompC</del>(CS1)-GFP without induction. In contrast, under the same conditions, we found no significant difference of GFP expression in <del class="diffchange diffchange-inline">PompC</del>(C)-GFP. Although, <del class="diffchange diffchange-inline">PompC</del>(WT)-GFP also doesn't show difference in expression of GFP at 4 hours of induction, there was slightly higher GFP expression 1.7-folds occurred in the wild type at 2 hours.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>After 4 hours of sucrose induction, transcriptional activity of <ins class="diffchange diffchange-inline">P''ompC</ins>(CB)<ins class="diffchange diffchange-inline">''</ins>-GFP and <ins class="diffchange diffchange-inline">P''ompC</ins>(CS1)<ins class="diffchange diffchange-inline">''</ins>-GFP increased 2.5-folds and 2.3-folds respectively. However, significant amount of leaky expression was found in <ins class="diffchange diffchange-inline">P''ompC</ins>(CS1)<ins class="diffchange diffchange-inline">''</ins>-GFP without induction. In contrast, under the same conditions, we found no significant difference of GFP expression in <ins class="diffchange diffchange-inline">P''ompC</ins>(C)<ins class="diffchange diffchange-inline">''</ins>-GFP. Although, <ins class="diffchange diffchange-inline">P''ompC</ins>(WT)<ins class="diffchange diffchange-inline">''</ins>-GFP also doesn't show difference in expression of GFP at 4 hours of induction, there was slightly higher GFP expression 1.7-folds occurred in the wild type at 2 hours.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Discussion==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Discussion==</div></td></tr>
</table>Vancouverhttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC&diff=207619&oldid=prevVancouver: /* Discussion */2010-10-28T03:32:47Z<p><span class="autocomment">Discussion</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Discussion==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Discussion==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The transcriptional activity of <del class="diffchange diffchange-inline">PompC</del>(WT)-GFP increased in particular period and declined after it reached the peak of expression. This result shows the relation to the study by the [http://partsregistry.org/Part:BBa_R0082:Experience Edinburgh iGEM 2009]. Moreover, we considereated that we can create varities of P''OmpC'' by combining OmpR binding sites on the upstream of the ''OmpC'' promoters with spacer sequence.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The transcriptional activity of <ins class="diffchange diffchange-inline">P''ompC</ins>(WT)<ins class="diffchange diffchange-inline">''</ins>-GFP increased in particular period and declined after it reached the peak of expression. This result shows the relation to the study by the [http://partsregistry.org/Part:BBa_R0082:Experience Edinburgh iGEM 2009]. Moreover, we considereated that we can create varities of P''OmpC'' by combining OmpR binding sites on the upstream of the ''OmpC'' promoters with spacer sequence.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Conclusion==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Conclusion==</div></td></tr>
</table>Vancouverhttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC&diff=207570&oldid=prevVancouver: /* Result */2010-10-28T03:31:23Z<p><span class="autocomment">Result</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*; Characterization of <del class="diffchange diffchange-inline">the </del>new series of ''OmpC'' promoters</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*; Characterization of new series of ''OmpC'' promoters</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Tokyotech_ompc_graph.jpg|thumb|center|400px|thumb|Fig.4-1-1 The induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA ]] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Tokyotech_ompc_graph.jpg|thumb|center|400px|thumb|Fig.4-1-1 The induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA ]] </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After 4 hours of sucrose induction, transcriptional activity of PompC(CB)-GFP and PompC(CS1)-GFP increased 2.5-folds and 2.3-folds respectively. However, significant amount of leaky expression was found in PompC(CS1)-GFP without induction. In contrast, under the same conditions, we found no significant difference of GFP expression in PompC(C)-GFP. Although, PompC(WT)-GFP also doesn't show difference in expression of GFP at 4 hours of induction, there was slightly higher GFP expression 1.7-folds occurred in the wild type at 2 hours.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After 4 hours of sucrose induction, transcriptional activity of PompC(CB)-GFP and PompC(CS1)-GFP increased 2.5-folds and 2.3-folds respectively. However, significant amount of leaky expression was found in PompC(CS1)-GFP without induction. In contrast, under the same conditions, we found no significant difference of GFP expression in PompC(C)-GFP. Although, PompC(WT)-GFP also doesn't show difference in expression of GFP at 4 hours of induction, there was slightly higher GFP expression 1.7-folds occurred in the wild type at 2 hours.</div></td></tr>
</table>Vancouverhttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC&diff=207415&oldid=prevVancouver: /* Result */2010-10-28T03:27:58Z<p><span class="autocomment">Result</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Tokyotech_ompc_graph.jpg|thumb|center|400px|thumb|Fig.4-1-1 The induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA ]] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Tokyotech_ompc_graph.jpg|thumb|center|400px|thumb|Fig.4-1-1 The induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA ]] </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After 4 hours of sucrose induction, transcriptional activity of PompC(CB)-GFP and PompC(CS1)-GFP increased 2.5-folds and 2.3-folds respectively. However, significant amount of leaky expression was found in PompC(CS1)-GFP without induction. In contrast, under the same conditions, we found no significant difference of GFP expression in PompC(C)-GFP. Although, PompC(WT)-GFP also doesn't show difference in expression of GFP at 4 hours of induction, there was slightly higher GFP expression 1.7-folds occurred in the wild type at 2 hours.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After 4 hours of sucrose induction, transcriptional activity of PompC(CB)-GFP and PompC(CS1)-GFP increased 2.5-folds and 2.3-folds respectively. However, significant amount of leaky expression was found in PompC(CS1)-GFP without induction. In contrast, under the same conditions, we found no significant difference of GFP expression in PompC(C)-GFP. Although, PompC(WT)-GFP also doesn't show difference in expression of GFP at 4 hours of induction, there was slightly higher GFP expression 1.7-folds occurred in the wild type at 2 hours.</div></td></tr>
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</table>Vancouverhttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC&diff=207392&oldid=prevVancouver: /* Result */2010-10-28T03:27:24Z<p><span class="autocomment">Result</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Tokyotech_ompc_graph.jpg|thumb|<del class="diffchange diffchange-inline">left</del>|400px|thumb|Fig.4-1-1 The induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA ]] </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Tokyotech_ompc_graph.jpg|thumb|<ins class="diffchange diffchange-inline">center</ins>|400px|thumb|Fig.4-1-1 The induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA ]] </div></td></tr>
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</table>Vancouverhttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC&diff=207290&oldid=prevVancouver: /* Result */2010-10-28T03:24:46Z<p><span class="autocomment">Result</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Tokyotech_ompc_graph.jpg|thumb|left|400px|thumb|Fig.4-1-1 The induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA ]] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Tokyotech_ompc_graph.jpg|thumb|left|400px|thumb|Fig.4-1-1 The induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA ]] </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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</table>Vancouverhttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC&diff=207208&oldid=prevVancouver: /* Result */2010-10-28T03:22:42Z<p><span class="autocomment">Result</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Result==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Result==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*;'''<del class="diffchange diffchange-inline">The </del>new series of ''OmpC'' promoters'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*;'''<ins class="diffchange diffchange-inline">Construction of </ins>new series of ''OmpC'' promoters'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:Tokyotech_New_pompC_construction.jpg|center|400px|thumb|Fig.4-1-4. PompC(WT) and the two-component system]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:Tokyotech_New_pompC_construction.jpg|center|400px|thumb|Fig.4-1-4. PompC(WT) and the two-component system]]</div></td></tr>
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</table>Vancouverhttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC&diff=206881&oldid=prevTaichi: /* Result */2010-10-28T03:14:53Z<p><span class="autocomment">Result</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*; Characterization of the new series of ''OmpC'' promoters</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*; Characterization of the new series of ''OmpC'' promoters</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Tokyotech_ompc_graph.jpg|thumb|left|400px|thumb|Fig.4-1-<del class="diffchange diffchange-inline">4 </del>The induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA ]] </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Tokyotech_ompc_graph.jpg|thumb|left|400px|thumb|Fig.4-1-<ins class="diffchange diffchange-inline">1 </ins>The induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA ]] </div></td></tr>
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</table>Taichihttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC&diff=206849&oldid=prevTaichi: /* Result */2010-10-28T03:14:10Z<p><span class="autocomment">Result</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Result==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Result==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*;'''The new series of ''OmpC'' promoters'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*;'''The new series of ''OmpC'' promoters'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[image:Tokyotech_New_pompC_construction.jpg|center|400px|PompC(WT) and the two-component system]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[image:Tokyotech_New_pompC_construction.jpg|center|400px|<ins class="diffchange diffchange-inline">thumb|Fig.4-1-4. </ins>PompC(WT) and the two-component system]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Three new promoters, P''ompC(CS1)'' BBa_395303, P''ompC(C)'' BBa_395301, P''ompC(CB)'' BBa_395302 were designed based on previous paper[1]. The second promoter, P''ompC(CS1)'', comprises only binding site1 fused to the canonical -35 and -10 region. P''ompC(C)'' is a composite of binding site 1 and additional 4 base pairs before the canonical -35 and -10 region. Likewise, P''ompC(CB)'' consists of binding site1 and the additional 10 base pairs before the -35 and -10 consensus sequence.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Three new promoters, P''ompC(CS1)'' BBa_395303, P''ompC(C)'' BBa_395301, P''ompC(CB)'' BBa_395302 were designed based on previous paper[1]. The second promoter, P''ompC(CS1)'', comprises only binding site1 fused to the canonical -35 and -10 region. P''ompC(C)'' is a composite of binding site 1 and additional 4 base pairs before the canonical -35 and -10 region. Likewise, P''ompC(CB)'' consists of binding site1 and the additional 10 base pairs before the -35 and -10 consensus sequence.</div></td></tr>
</table>Taichihttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC&diff=206783&oldid=prevTaichi: /* Result */2010-10-28T03:12:44Z<p><span class="autocomment">Result</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*; Characterization of the new series of ''OmpC'' promoters</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*; Characterization of the new series of ''OmpC'' promoters</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Tokyotech_ompc_graph.jpg|thumb|left|400px|Fig. 4-1-<del class="diffchange diffchange-inline">1 </del>The induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA ]] </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Tokyotech_ompc_graph.jpg|thumb|left|400px<ins class="diffchange diffchange-inline">|thumb</ins>|Fig.4-1-<ins class="diffchange diffchange-inline">4 </ins>The induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA ]] </div></td></tr>
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</table>Taichi