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Team:Tokyo Tech/Project/wolf coli
From 2010.igem.org
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==Wolf coli== | ==Wolf coli== | ||
==Overview of new series of ''OmpC'' promoters== | ==Overview of new series of ''OmpC'' promoters== | ||
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We constructed BioBrick parts of the new series of osmoregulative promoters which are derivatives of the wild type OmpC promoter. The new series of promoters are PompC(C), PompC(CB) and PompC(CS1). In order to measure the strength of each promoter we used GFP as a reporter. We found that expression of GFP in OmpC(CB) and OmpC(CS1) promoters increased in high osmolarity medium comparing with the expression in low osmolarity medium. In contrast, under same conditions, there is no significant difference of GFP expression in OmpC(C) and OmpC(WT) promoters. | We constructed BioBrick parts of the new series of osmoregulative promoters which are derivatives of the wild type OmpC promoter. The new series of promoters are PompC(C), PompC(CB) and PompC(CS1). In order to measure the strength of each promoter we used GFP as a reporter. We found that expression of GFP in OmpC(CB) and OmpC(CS1) promoters increased in high osmolarity medium comparing with the expression in low osmolarity medium. In contrast, under same conditions, there is no significant difference of GFP expression in OmpC(C) and OmpC(WT) promoters. | ||
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| + | =New BioBrick Parts= | ||
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| + | We succeeded in designing 2 news osmoregulative promoters, OmpC(CB) and OmpC(CS1), which can also be utilized in light sensitive system. | ||
| + | [[Image:Van_OmpC_graph.jpg|frame|200px|left|Figure 1. The induction of new'' OmpC'' series in high osmolarity medium ]] | ||
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| + | =Introduction= | ||
| + | #=What is OmpC promoter?=# | ||
| + | #=The new series of OmpC promoters# | ||
| + | =Material and Method= | ||
| + | #=Construction of plasmid=# | ||
| + | |||
| + | |||
Revision as of 08:17, 25 October 2010
Contents |
Wolf coli
Overview of new series of OmpC promoters
We constructed BioBrick parts of the new series of osmoregulative promoters which are derivatives of the wild type OmpC promoter. The new series of promoters are PompC(C), PompC(CB) and PompC(CS1). In order to measure the strength of each promoter we used GFP as a reporter. We found that expression of GFP in OmpC(CB) and OmpC(CS1) promoters increased in high osmolarity medium comparing with the expression in low osmolarity medium. In contrast, under same conditions, there is no significant difference of GFP expression in OmpC(C) and OmpC(WT) promoters.
New BioBrick Parts
We succeeded in designing 2 news osmoregulative promoters, OmpC(CB) and OmpC(CS1), which can also be utilized in light sensitive system.
Figure 1. The induction of new OmpC series in high osmolarity medium
Introduction
- =What is OmpC promoter?=#
- =The new series of OmpC promoters#
Material and Method
- =Construction of plasmid=#
