Team:Tokyo Tech/Parts
From 2010.igem.org
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<!-- ここから書き始めて下さい--> | <!-- ここから書き始めて下さい--> | ||
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==New parts which were characterized and worked as designed== | ==New parts which were characterized and worked as designed== | ||
+ | '''Apple Reporter''' | ||
{| style="margin: 1em auto 1em auto" width="600px" | {| style="margin: 1em auto 1em auto" width="600px" | ||
- | |- style="background-color:# | + | |- style="background-color:#4169e1;" |
!Name||Part type||Registry Link | !Name||Part type||Registry Link | ||
- | |- style="background-color:# | + | |- style="background-color:#f5fffa;" |
|MpAAT1||Generator||<partinfo>BBa_K395602</partinfo> | |MpAAT1||Generator||<partinfo>BBa_K395602</partinfo> | ||
- | |- style="background-color:# | + | |- style="background-color:#f5fffa;" |
|rbs-crtW||Translational unit||<partinfo>BBa_K395705</partinfo> | |rbs-crtW||Translational unit||<partinfo>BBa_K395705</partinfo> | ||
- | |- style="background-color:# | + | |- style="background-color:#f5fffa;" |
- | |pBad/araC-crtZW||Generator||<partinfo>BBa_K395706</partinfo> | + | |pBad/araC-crtZW||Generator||<partinfo>BBa_K395706</partinfo> |
|- | |- | ||
|} | |} | ||
+ | '''Artificial Cooperation System''' | ||
{| style="margin: 1em auto 1em auto" width="600px" | {| style="margin: 1em auto 1em auto" width="600px" | ||
- | |- style="background-color:# | + | |- style="background-color:#4169e1;" |
!Name||Part type||Registry Link | !Name||Part type||Registry Link | ||
+ | |- style="background-color:#f5fffa;" | ||
|(PluxR rep weak-GFP) improved||Reporter||<partinfo>BBa_K395105</partinfo> | |(PluxR rep weak-GFP) improved||Reporter||<partinfo>BBa_K395105</partinfo> | ||
- | |- style="background-color:# | + | |- style="background-color:#f5fffa;" |
- | |PluxR act-Cm|| | + | |PluxR act-Cm||signalling||<partinfo>BBa_K395162</partinfo> |
|- | |- | ||
|} | |} | ||
+ | '''"Wolfcoli"zation''' | ||
{| style="margin: 1em auto 1em auto" width="600px" | {| style="margin: 1em auto 1em auto" width="600px" | ||
- | |- style="background-color:# | + | |- style="background-color:#4169e1;" |
!Name||Part type||Registry Link | !Name||Part type||Registry Link | ||
- | |- style="background-color:# | + | |- style="background-color:#f5fffa;" |
- | |PompC(CB)-GFP improved | + | |PompC(CB)-GFP improved||Reporter||<partinfo>BBa_K395305</partinfo> |
- | |- style="background-color:# | + | |- style="background-color:#f5fffa;" |
- | |PompC( | + | |PompC(CS1)-GFP||Reporter||<partinfo>BBa_K395306</partinfo> |
|- | |- | ||
|} | |} | ||
==New parts which were characterized and did not worked as designed== | ==New parts which were characterized and did not worked as designed== | ||
+ | '''"Wolfcoli"zation''' | ||
{| style="margin: 1em auto 1em auto" width="600px" | {| style="margin: 1em auto 1em auto" width="600px" | ||
- | |- style="background-color:# | + | |- style="background-color:#4169e1;" |
!Name||Part type||Registry Link | !Name||Part type||Registry Link | ||
- | |- style="background-color:# | + | |- style="background-color:#f5fffa;" |
|PompC(C)-GFP ||Reporter||<partinfo>BBa_K395304</partinfo> | |PompC(C)-GFP ||Reporter||<partinfo>BBa_K395304</partinfo> | ||
- | |- style="background-color:# | + | |- style="background-color:#f5fffa;" |
|PompC(WT)-GFP ||Reporter||<partinfo>BBa_K395307</partinfo> | |PompC(WT)-GFP ||Reporter||<partinfo>BBa_K395307</partinfo> | ||
|- | |- | ||
Line 53: | Line 57: | ||
==Existing parts which were characterized through our activity== | ==Existing parts which were characterized through our activity== | ||
+ | |||
+ | '''Apple Reporter ''' | ||
{| style="margin: 1em auto 1em auto" width="600px" | {| style="margin: 1em auto 1em auto" width="600px" | ||
- | |- style="background-color:# | + | |- style="background-color:#4169e1;" |
!Name||Part type||Registry Link | !Name||Part type||Registry Link | ||
- | |- style="background-color:# | + | |- style="background-color:#f5fffa;" |
- | | | + | |crtZ (beta-carotene hydroxylase) coding sequence ||Coding||<partinfo>BBa_I742157</partinfo> |
- | + | |- style="background-color:#f5fffa;" | |
- | | | + | |crtZ (beta-carotene hydroxylase) with native rbs ||Translational Unit||<partinfo>BBa_I742158</partinfo> |
- | |- style="background-color:# | + | |
- | | | + | |
|- | |- | ||
|} | |} | ||
+ | |||
+ | ''In order to characterize these parts, following 3 improved parts were constructed.'' | ||
+ | |||
+ | :::::<br>(Name,Parts,registry Number) | ||
+ | :::::<br>(placIQ-crtZ ,Generator,<partinfo>BBa_K395701</partinfo>) | ||
+ | :::::<br>(pBad/araC-crtZ ,Generator,<partinfo>BBa_K395702</partinfo>) | ||
+ | :::::<br>(pBad/araC-crtZ-crtEBIY ,Generator,<partinfo>BBa_K395704</partinfo>) | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | '''“Wolfcoli”zation ''' | ||
{| style="margin: 1em auto 1em auto" width="600px" | {| style="margin: 1em auto 1em auto" width="600px" | ||
- | |- style="background-color:# | + | |- style="background-color:#4169e1;" |
!Name||Part type||Registry Link | !Name||Part type||Registry Link | ||
- | |- style="background-color:#4169e1;" | + | |- style="background-color:#f5fffa;" |
+ | |LacI-Mutant ||Coding||<partinfo>BBa_K082005</partinfo> | ||
+ | |- style="background-color:#f5fffa;" | ||
+ | |LacIM1-B0015||Composite||<partinfo>BBa_K082016</partinfo> | ||
+ | |- style="background-color:#f5fffa;" | ||
+ | |B0034-LacIM1-B0015||Generator||<partinfo>BBa_K082026</partinfo> | ||
+ | |- | ||
+ | |} | ||
+ | |||
+ | ''In order to characterize these parts, following 2 improved parts were constructed.'' | ||
+ | |||
+ | :::::<br>(Name,Parts,registry Number) | ||
+ | :::::<br>(Pbad/araC-lacIM1 ,Composite,<partinfo>BBa_K395401</partinfo>) | ||
+ | :::::<br>(Pbad/araC-lacI ,Composite,<partinfo>BBa_K395402</partinfo>) | ||
+ | |||
+ | |||
+ | '''Artificial Cooperation System''' | ||
+ | {| style="margin: 1em auto 1em auto" width="600px" | ||
+ | |- style="background-color:#4169e1;" | ||
+ | !Name||Part type||Registry Link | ||
+ | |- style="background-color:#f5fffa;" | ||
|PluxR rep||Regulatory||<partinfo>BBa_R0061</partinfo> | |PluxR rep||Regulatory||<partinfo>BBa_R0061</partinfo> | ||
- | |||
- | |||
|- | |- | ||
|} | |} | ||
+ | ''In order to characterize this part, following improved part was constructed.'' | ||
+ | :::::<br>(Name,Parts,registry Number) | ||
+ | :::::<br>(GFP reporter repressed by LuxR and 3OC6HSL ,Reporter,<partinfo>BBa_K395101</partinfo>) | ||
{| style="margin: 1em auto 1em auto" width="600px" | {| style="margin: 1em auto 1em auto" width="600px" | ||
- | |- style="background-color:# | + | |- style="background-color:#4169e1;" |
!Name||Part type||Registry Link | !Name||Part type||Registry Link | ||
- | |- style="background-color:# | + | |- style="background-color:#f5fffa;" |
- | | | + | |PluxR act||Regulatory||<partinfo>BBa_R0062</partinfo> |
- | + | ||
- | + | ||
|- | |- | ||
|} | |} | ||
+ | ''In order to characterize this part, following part was constructed.'' | ||
+ | :::::<br>(Name,Parts,registry Number) | ||
+ | :::::<br>(GFP reporter activated by LuxR and 3OC6HSL,Reporter,<partinfo>BBa_K395100</partinfo>) | ||
+ | ==All of the parts we registered== | ||
+ | <groupparts>iGEM010 Tokyo_Tech</groupparts> | ||
- | |||
<!-- ココより下はいじっちゃやーよ--> | <!-- ココより下はいじっちゃやーよ--> |
Latest revision as of 22:58, 27 October 2010
New parts which were characterized and worked as designed
Apple Reporter
Name | Part type | Registry Link |
---|---|---|
MpAAT1 | Generator | <partinfo>BBa_K395602</partinfo> |
rbs-crtW | Translational unit | <partinfo>BBa_K395705</partinfo> |
pBad/araC-crtZW | Generator | <partinfo>BBa_K395706</partinfo> |
Artificial Cooperation System
Name | Part type | Registry Link |
---|---|---|
(PluxR rep weak-GFP) improved | Reporter | <partinfo>BBa_K395105</partinfo> |
PluxR act-Cm | signalling | <partinfo>BBa_K395162</partinfo> |
"Wolfcoli"zation
Name | Part type | Registry Link |
---|---|---|
PompC(CB)-GFP improved | Reporter | <partinfo>BBa_K395305</partinfo> |
PompC(CS1)-GFP | Reporter | <partinfo>BBa_K395306</partinfo> |
New parts which were characterized and did not worked as designed
"Wolfcoli"zation
Name | Part type | Registry Link |
---|---|---|
PompC(C)-GFP | Reporter | <partinfo>BBa_K395304</partinfo> |
PompC(WT)-GFP | Reporter | <partinfo>BBa_K395307</partinfo> |
Existing parts which were characterized through our activity
Apple Reporter
Name | Part type | Registry Link |
---|---|---|
crtZ (beta-carotene hydroxylase) coding sequence | Coding | <partinfo>BBa_I742157</partinfo> |
crtZ (beta-carotene hydroxylase) with native rbs | Translational Unit | <partinfo>BBa_I742158</partinfo> |
In order to characterize these parts, following 3 improved parts were constructed.
(Name,Parts,registry Number)
(placIQ-crtZ ,Generator,<partinfo>BBa_K395701</partinfo>)
(pBad/araC-crtZ ,Generator,<partinfo>BBa_K395702</partinfo>)
(pBad/araC-crtZ-crtEBIY ,Generator,<partinfo>BBa_K395704</partinfo>)
“Wolfcoli”zation
Name | Part type | Registry Link |
---|---|---|
LacI-Mutant | Coding | <partinfo>BBa_K082005</partinfo> |
LacIM1-B0015 | Composite | <partinfo>BBa_K082016</partinfo> |
B0034-LacIM1-B0015 | Generator | <partinfo>BBa_K082026</partinfo> |
In order to characterize these parts, following 2 improved parts were constructed.
(Name,Parts,registry Number)
(Pbad/araC-lacIM1 ,Composite,<partinfo>BBa_K395401</partinfo>)
(Pbad/araC-lacI ,Composite,<partinfo>BBa_K395402</partinfo>)
Artificial Cooperation System
Name | Part type | Registry Link |
---|---|---|
PluxR rep | Regulatory | <partinfo>BBa_R0061</partinfo> |
In order to characterize this part, following improved part was constructed.
(Name,Parts,registry Number)
(GFP reporter repressed by LuxR and 3OC6HSL ,Reporter,<partinfo>BBa_K395101</partinfo>)
Name | Part type | Registry Link |
---|---|---|
PluxR act | Regulatory | <partinfo>BBa_R0062</partinfo> |
In order to characterize this part, following part was constructed.
(Name,Parts,registry Number)
(GFP reporter activated by LuxR and 3OC6HSL,Reporter,<partinfo>BBa_K395100</partinfo>)
All of the parts we registered
<groupparts>iGEM010 Tokyo_Tech</groupparts>
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