Team:Tokyo Tech/Parts

From 2010.igem.org

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<!--  ここから書き始めて下さい-->
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==Index==
+
 
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<groupparts>iGEM010 Tokyo_Tech</groupparts>
+
==New parts which were characterized and worked as designed==
==New parts which were characterized and worked as designed==
 +
'''Apple Reporter'''
{| style="margin: 1em auto 1em auto" width="600px"
{| style="margin: 1em auto 1em auto" width="600px"
-
  |- style="background-color:#c2a4e9;"  
+
  |- style="background-color:#4169e1;"  
!Name||Part type||Registry Link
!Name||Part type||Registry Link
-
|- style="background-color:#4169e1;"  
+
|- style="background-color:#f5fffa;"  
|MpAAT1||Generator||<partinfo>BBa_K395602</partinfo>
|MpAAT1||Generator||<partinfo>BBa_K395602</partinfo>
-
|- style="background-color:#4169e1;"  
+
|- style="background-color:#f5fffa;"  
|rbs-crtW||Translational unit||<partinfo>BBa_K395705</partinfo>
|rbs-crtW||Translational unit||<partinfo>BBa_K395705</partinfo>
-
|- style="background-color:#4169e1;"  
+
|- style="background-color:#f5fffa;"  
-
|pBad/araC-crtZW||Generator||<partinfo>BBa_K395706</partinfo>)
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|pBad/araC-crtZW||Generator||<partinfo>BBa_K395706</partinfo>
|-
|-
|}
|}
 +
'''Artificial Cooperation System'''
{| style="margin: 1em auto 1em auto" width="600px"
{| style="margin: 1em auto 1em auto" width="600px"
-
|- style="background-color:#c2a4e9;"  
+
|- style="background-color:#4169e1;"  
!Name||Part type||Registry Link  
!Name||Part type||Registry Link  
 +
|- style="background-color:#f5fffa;"
|(PluxR rep weak-GFP) improved||Reporter||<partinfo>BBa_K395105</partinfo>
|(PluxR rep weak-GFP) improved||Reporter||<partinfo>BBa_K395105</partinfo>
-
|- style="background-color:#4169e1;"  
+
|- style="background-color:#f5fffa;"  
-
|PluxR act-Cm||Coding||<partinfo>BBa_K395162</partinfo>
+
|PluxR act-Cm||signalling||<partinfo>BBa_K395162</partinfo>
|-
|-
|}
|}
 +
'''"Wolfcoli"zation'''
{| style="margin: 1em auto 1em auto" width="600px"
{| style="margin: 1em auto 1em auto" width="600px"
-
  |- style="background-color:#c2a4e9;"  
+
  |- style="background-color:#4169e1;"  
!Name||Part type||Registry Link
!Name||Part type||Registry Link
-
|- style="background-color:#4169e1;"  
+
|- style="background-color:#f5fffa;"  
-
|PompC(CB)-GFP improved)||Reporter||<partinfo>BBa_K395305</partinfo>
+
|PompC(CB)-GFP improved||Reporter||<partinfo>BBa_K395305</partinfo>
-
|- style="background-color:#4169e1;"  
+
|- style="background-color:#f5fffa;"  
-
|PompC(CS-1)-GFP||Reporter||<partinfo>BBa_K395306</partinfo>
+
|PompC(CS1)-GFP||Reporter||<partinfo>BBa_K395306</partinfo>
|-
|-
|}
|}
==New parts which were characterized and did not worked as designed==
==New parts which were characterized and did not worked as designed==
 +
'''"Wolfcoli"zation'''
{| style="margin: 1em auto 1em auto" width="600px"
{| style="margin: 1em auto 1em auto" width="600px"
-
  |- style="background-color:#c2a4e9;"  
+
  |- style="background-color:#4169e1;"  
!Name||Part type||Registry Link
!Name||Part type||Registry Link
-
|- style="background-color:#4169e1;"  
+
|- style="background-color:#f5fffa;"  
|PompC(C)-GFP ||Reporter||<partinfo>BBa_K395304</partinfo>
|PompC(C)-GFP ||Reporter||<partinfo>BBa_K395304</partinfo>
-
|- style="background-color:#4169e1;"  
+
|- style="background-color:#f5fffa;"  
|PompC(WT)-GFP ||Reporter||<partinfo>BBa_K395307</partinfo>
|PompC(WT)-GFP ||Reporter||<partinfo>BBa_K395307</partinfo>
|-
|-
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==Existing parts which were characterized through our activity==
==Existing parts which were characterized through our activity==
 +
 +
'''Apple Reporter '''
{| style="margin: 1em auto 1em auto" width="600px"
{| style="margin: 1em auto 1em auto" width="600px"
-
  |- style="background-color:#c2a4e9;"  
+
  |- style="background-color:#4169e1;"  
!Name||Part type||Registry Link
!Name||Part type||Registry Link
-
|- style="background-color:#4169e1;"  
+
|- style="background-color:#f5fffa;"  
-
|placIQ-crtZ ||Generator||<partinfo>BBa_K395701</partinfo>
+
|crtZ (beta-carotene hydroxylase) coding sequence ||Coding||<partinfo>BBa_I742157</partinfo>
-
|- style="background-color:#4169e1;"
+
|- style="background-color:#f5fffa;"  
-
|pBad/araC-crtZ ||Generator||<partinfo>BBa_K395702</partinfo>
+
|crtZ (beta-carotene hydroxylase) with native rbs ||Translational Unit||<partinfo>BBa_I742158</partinfo>
-
|- style="background-color:#4169e1;"  
+
-
|pBad/araC-crtZ-crtEBIY ||Generator||<partinfo>BBa_K395704</partinfo>
+
|-
|-
|}
|}
 +
 +
''In order to characterize these parts, following 3 improved parts were constructed.''
 +
 +
:::::<br>(Name,Parts,registry Number)
 +
:::::<br>(placIQ-crtZ ,Generator,<partinfo>BBa_K395701</partinfo>)
 +
:::::<br>(pBad/araC-crtZ ,Generator,<partinfo>BBa_K395702</partinfo>)
 +
:::::<br>(pBad/araC-crtZ-crtEBIY ,Generator,<partinfo>BBa_K395704</partinfo>)
 +
 +
 +
 +
 +
'''“Wolfcoli”zation  '''
{| style="margin: 1em auto 1em auto" width="600px"
{| style="margin: 1em auto 1em auto" width="600px"
-
  |- style="background-color:#c2a4e9;"  
+
  |- style="background-color:#4169e1;"  
!Name||Part type||Registry Link
!Name||Part type||Registry Link
-
|- style="background-color:#4169e1;"  
+
|- style="background-color:#f5fffa;"
 +
|LacI-Mutant ||Coding||<partinfo>BBa_K082005</partinfo>
 +
|- style="background-color:#f5fffa;"
 +
|LacIM1-B0015||Composite||<partinfo>BBa_K082016</partinfo>
 +
|- style="background-color:#f5fffa;"
 +
|B0034-LacIM1-B0015||Generator||<partinfo>BBa_K082026</partinfo>
 +
|-
 +
|}
 +
 
 +
''In order to characterize these parts, following 2 improved parts were constructed.''
 +
 
 +
:::::<br>(Name,Parts,registry Number)
 +
:::::<br>(Pbad/araC-lacIM1 ,Composite,<partinfo>BBa_K395401</partinfo>)
 +
:::::<br>(Pbad/araC-lacI ,Composite,<partinfo>BBa_K395402</partinfo>)
 +
 
 +
 
 +
'''Artificial Cooperation System'''
 +
{| style="margin: 1em auto 1em auto" width="600px"
 +
|- style="background-color:#4169e1;"
 +
!Name||Part type||Registry Link
 +
|- style="background-color:#f5fffa;"  
|PluxR rep||Regulatory||<partinfo>BBa_R0061</partinfo>
|PluxR rep||Regulatory||<partinfo>BBa_R0061</partinfo>
-
|- style="background-color:#4169e1;"
 
-
|PluxR act||Regulatory||<partinfo>BBa_R0062</partinfo>
 
|-
|-
|}
|}
 +
''In order to characterize this part, following improved part was constructed.''
 +
:::::<br>(Name,Parts,registry Number)
 +
:::::<br>(GFP reporter repressed by LuxR and 3OC6HSL ,Reporter,<partinfo>BBa_K395101</partinfo>)
{| style="margin: 1em auto 1em auto" width="600px"
{| style="margin: 1em auto 1em auto" width="600px"
-
  |- style="background-color:#c2a4e9;"  
+
  |- style="background-color:#4169e1;"  
!Name||Part type||Registry Link
!Name||Part type||Registry Link
-
|- style="background-color:#4169e1;"  
+
|- style="background-color:#f5fffa;"  
-
|Pbad/araC-lacIM1 ||Composite||<partinfo>BBa_K395401</partinfo>
+
|PluxR act||Regulatory||<partinfo>BBa_R0062</partinfo>
-
|- style="background-color:#4169e1;"
+
-
|Pbad/araC-lacI ||Composite||<partinfo>BBa_K395402</partinfo>
+
|-
|-
|}
|}
 +
''In order to characterize this part, following part was constructed.''
 +
:::::<br>(Name,Parts,registry Number)
 +
:::::<br>(GFP reporter activated by LuxR and 3OC6HSL,Reporter,<partinfo>BBa_K395100</partinfo>)
 +
==All of the parts we registered==
 +
<groupparts>iGEM010 Tokyo_Tech</groupparts>
-
<groupparts>iGEM010 Tokyo_Tech</groupparts>
 
<!-- ココより下はいじっちゃやーよ-->
<!-- ココより下はいじっちゃやーよ-->

Latest revision as of 22:58, 27 October 2010

iGEM Tokyo Tech 2010 "E.coli with Humanity"


New parts which were characterized and worked as designed

Apple Reporter

NamePart typeRegistry Link
MpAAT1Generator<partinfo>BBa_K395602</partinfo>
rbs-crtWTranslational unit<partinfo>BBa_K395705</partinfo>
pBad/araC-crtZWGenerator<partinfo>BBa_K395706</partinfo>

Artificial Cooperation System

NamePart typeRegistry Link
(PluxR rep weak-GFP) improvedReporter<partinfo>BBa_K395105</partinfo>
PluxR act-Cmsignalling<partinfo>BBa_K395162</partinfo>

"Wolfcoli"zation

NamePart typeRegistry Link
PompC(CB)-GFP improvedReporter<partinfo>BBa_K395305</partinfo>
PompC(CS1)-GFPReporter<partinfo>BBa_K395306</partinfo>

New parts which were characterized and did not worked as designed

"Wolfcoli"zation

NamePart typeRegistry Link
PompC(C)-GFP Reporter<partinfo>BBa_K395304</partinfo>
PompC(WT)-GFP Reporter<partinfo>BBa_K395307</partinfo>

Existing parts which were characterized through our activity

Apple Reporter

NamePart typeRegistry Link
crtZ (beta-carotene hydroxylase) coding sequence Coding<partinfo>BBa_I742157</partinfo>
crtZ (beta-carotene hydroxylase) with native rbs Translational Unit<partinfo>BBa_I742158</partinfo>

In order to characterize these parts, following 3 improved parts were constructed.


(Name,Parts,registry Number)

(placIQ-crtZ ,Generator,<partinfo>BBa_K395701</partinfo>)

(pBad/araC-crtZ ,Generator,<partinfo>BBa_K395702</partinfo>)

(pBad/araC-crtZ-crtEBIY ,Generator,<partinfo>BBa_K395704</partinfo>)



“Wolfcoli”zation

NamePart typeRegistry Link
LacI-Mutant Coding<partinfo>BBa_K082005</partinfo>
LacIM1-B0015Composite<partinfo>BBa_K082016</partinfo>
B0034-LacIM1-B0015Generator<partinfo>BBa_K082026</partinfo>

In order to characterize these parts, following 2 improved parts were constructed.


(Name,Parts,registry Number)

(Pbad/araC-lacIM1 ,Composite,<partinfo>BBa_K395401</partinfo>)

(Pbad/araC-lacI ,Composite,<partinfo>BBa_K395402</partinfo>)


Artificial Cooperation System

NamePart typeRegistry Link
PluxR repRegulatory<partinfo>BBa_R0061</partinfo>

In order to characterize this part, following improved part was constructed.


(Name,Parts,registry Number)

(GFP reporter repressed by LuxR and 3OC6HSL ,Reporter,<partinfo>BBa_K395101</partinfo>)
NamePart typeRegistry Link
PluxR actRegulatory<partinfo>BBa_R0062</partinfo>

In order to characterize this part, following part was constructed.


(Name,Parts,registry Number)

(GFP reporter activated by LuxR and 3OC6HSL,Reporter,<partinfo>BBa_K395100</partinfo>)

All of the parts we registered

<groupparts>iGEM010 Tokyo_Tech</groupparts>


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