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From 2010.igem.org

Contents 1 2010/10/12 1.1 1：DNA extraction 1.2 2：DNA Digestion 1.3 3:PCR 1.4 4:Plasmid extraction from E-coli 1.5 5：DNA Ligation 1.6 3：DNA Digestion

2010/10/12

1：DNA extraction

＜Member＞
Mariko

＜Sample＞

• 1-2
• 10-2
• 2

＜Protocol＞
See Protocol 4

2：DNA Digestion

＜Member＞
Mariko

＜Sample, Materials＞
・PCR product

• 1-2

・Digest enzyme

• AvrⅡ

＜Protocol＞
See Prptpcpl 9

3:PCR

<Member>
nito

<Sample>

• 9
• 1-2-3
• 4-5

<Protocol>
See Protocol 2

4:Plasmid extraction from E-coli

<Member>
Takahiro

<Sample>

• 1-2
• 1-3
• 1-5
• 1-6

<Protocol>
See Protocol 6

5：DNA Ligation

＜Member＞
nito

＜Sample＞

• 1-2
• 10-2

＜Protocol＞
See Protocol 10

3：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

• 9H
• 1-2
• 10-2

・Digest enzyme

• AvrⅡ
• NheⅠ
• SpeⅠ

＜Protocol＞
See Protocol 9