Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/12

From 2010.igem.org

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===4:Plasmid extraction from E-coli===
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taka
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Takahiro
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<Sample><br />

Revision as of 10:42, 16 October 2010

Contents

2010/10/12

1:DNA extraction

<Member>
Mariko

<Sample>

  • 1-2
  • 10-2
  • 2

<Protocol>
See Protocol 4

2:DNA Digestion

<Member>
Mariko

<Sample, Materials>
・PCR product

  • 1-2

・Digest enzyme

  • AvrⅡ

<Protocol>
See Prptpcpl 9

3:PCR

<Member>
nito

<Sample>

  • 9
  • 1-2-3
  • 4-5

<Protocol>
See Protocol 2

4:Plasmid extraction from E-coli

<Member>
Takahiro

<Sample>

  • 1-2
  • 1-3
  • 1-5
  • 1-6

<Protocol>
See Protocol 6

5:DNA Ligation

<Member>
nito

<Sample>

  • 1-2
  • 10-2


<Protocol>
See Protocol 10

3:DNA Digestion

<Member>
nito


<Sample, Materials>
・PCR productions

  • 9H
  • 1-2
  • 10-2

・Digest enzyme

  • AvrⅡ
  • NheⅠ
  • SpeⅠ


<Protocol>
See Protocol 9