Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/12

From 2010.igem.org

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<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
 +
 +
===2:Plasmid extraction from E-coli===
 +
<Member><br />
 +
mizuki
 +
 +
<Sample><br />
 +
*Promotor
 +
*RBS
 +
 +
<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ]
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 +
===5：DNA Ligation ===
 +
＜Member＞<br />
 +
mio,mizuki
 +
 +
＜Sample＞<br />
 +
*1-2 
 +
*4-5
 +
*6A-7
 +
*7-8-10
 +
(after digestion)
 +
 +
＜Protocol＞<br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
 +
 +
==3：DNA Digestion==
 +
＜Member＞<br />
 +
 +
 +
 +
＜Sample, Materials＞<br />
 +
・PCR productions
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*1L(8/25)  8μl
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*2L(8/26)  8μl
 +
*4H(8/26)  16μl
 +
*5H(8/26)  16μl
 +
 +
・Digest enzyme
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*AvrⅡ
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*NheⅠ
 +
*SpeⅠ
 +
 +
 +
＜Protocol＞<br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Protocol 9 ]

Revision as of 10:19, 16 October 2010

Contents

2010/10/12

1：DNA extraction

＜Member＞
Mariko

＜Sample＞

  • 1-2
  • 10-2
  • 2

＜Protocol＞
See Protocol 4

2：DNA Digestion

＜Member＞
Mariko

＜Sample, Materials＞
・PCR product

  • 1-2

・Digest enzyme

  • Avr2

＜Protocol＞
See Prptpcpl 9

3:PCR

<Member>
nito

<Sample>

  • 9
  • 1-2-3
  • 4-5

<Protocol>
See Protocol 2

2:Plasmid extraction from E-coli

<Member>
mizuki

<Sample>

  • Promotor
  • RBS

<Protocol>
See Protocol 6

5：DNA Ligation

＜Member＞
mio,mizuki

＜Sample＞

  • 1-2
  • 4-5
  • 6A-7
  • 7-8-10

(after digestion)

＜Protocol＞
See Protocol 10

3：DNA Digestion

＜Member＞


＜Sample, Materials＞
・PCR productions

  • 1L(8/25) 8μl
  • 2L(8/26) 8μl
  • 4H(8/26) 16μl
  • 5H(8/26) 16μl

・Digest enzyme

  • AvrⅡ
  • NheⅠ
  • SpeⅠ


＜Protocol＞
See Protocol 9

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/10/12"