Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/03

From 2010.igem.org


E.coli Fiber Project Notebook

August 2010
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2010/10/03 Sunday (Naoto)

Experiment:PCR of bcsA(A.xylinum)

member
naoto and watachin
marerial
  • bcsA(A.xylinum)
If you want to know other materials, see protocol3
procedure
see protocol3


Experiment:Electrophoresis of bcsC,D(A.xylinum),bcsA,B,C,EFG,Q(E.coli),and pSB13

member
naoto and watachin
marerial
  • bcsC,D(A.xylinum),bcsA,B,C,EFG,Q(E.coli),pSB13
If you want to know other materials, see protocol4


procedure
see protocol4


Experiment:Miniprep of BBa_K145001

member
naoto and watachin
marerial
  • Preculture of E.coli having BBa_K145001
If you want to know other materials, see protocol9


procedure
see protocol9


Experiment:Ligation of pSB1C3 and bcsC,D(A.xylinum),bcsA(E.coli)

member
naoto and watachin
marerial
  • pSB1C3
  • bcsC,D(A.xylinum),bcsA(E.coli)
If you want to know other materials, see protocol7


procedure
see protocol4


Experiment:Transformation of parts of bcsC,D(A.xylinum),bcsA(E.coli)

member
naoto and watachin
marerial
  • parts of bcsC,D(A.xylinum),bcsA(E.coli)
If you want to know other materials, see protocol8


procedure
see protocol8


Experiment:Electrophoresis of bcsA(A.xylinum)

member
naoto and watachin
marerial
  • bcsA(A.xylinum)
If you want to know other materials, see protocol4


procedure
see protocol4


result
Band of bcsA could be seen clearly

Experiment:Purification of bcsA(A.xylinum)

member
naoto and watachin
marerial
  • bcsA(A.xylinum) in agarose gel
If you want to know other materials, see protocol5


procedure
see protocol4 protocol4