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Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/03
From 2010.igem.org
E.coli Fiber Project Notebook
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2010/10/03 Sunday (Naoto)
Experiment:PCR of bcsA(A.xylinum)
- member
- naoto and watachin
- marerial
- bcsA(A.xylinum)
- If you want to know other materials, see protocol3
- procedure
- see protocol3
Experiment:Electrophoresis of bcsC,D(A.xylinum),bcsA,B,C,EFG,Q(E.coli),and pSB13
- member
- naoto and watachin
- marerial
- bcsC,D(A.xylinum),bcsA,B,C,EFG,Q(E.coli),pSB13
- If you want to know other materials, see protocol4
- procedure
- see protocol4
Experiment:Miniprep of BBa_K145001
- member
- naoto and watachin
- marerial
- Preculture of E.coli having BBa_K145001
- If you want to know other materials, see protocol9
- procedure
- see protocol9
Experiment:Ligation of pSB1C3 and bcsC,D(A.xylinum),bcsA(E.coli)
- member
- naoto and watachin
- marerial
- pSB1C3
- bcsC,D(A.xylinum),bcsA(E.coli)
- If you want to know other materials, see protocol7
- procedure
- see protocol4
Experiment:Transformation of parts of bcsC,D(A.xylinum),bcsA(E.coli)
- member
- naoto and watachin
- marerial
- parts of bcsC,D(A.xylinum),bcsA(E.coli)
- If you want to know other materials, see protocol8
- procedure
- see protocol8
Experiment:Electrophoresis of bcsA(A.xylinum)
- member
- naoto and watachin
- marerial
- bcsA(A.xylinum)
- If you want to know other materials, see protocol4
- procedure
- see protocol4
- result
- Band of bcsA could be seen clearly
Experiment:Purification of bcsA(A.xylinum)
- member
- naoto and watachin
- marerial
- bcsA(A.xylinum) in agarose gel
- If you want to know other materials, see protocol5
- procedure
- see protocol4 protocol4
