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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/28
From 2010.igem.org
E.coli Fiber Project Notebook
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2010/09/28 Tuesday (Naoto)
Experiment:Electrophoresis of insert check PCR
member
naoto and bambi75
material
- PCR production (bcsD(A.xylinum) )
If you want to know other materials,refer to protocol4
procedure
see protocol4
result Bands didn't appear at all
Experiment:Electrophoresis of bcsEFG,Q
member
naoto and bambi75
material
- PCR production (bcsEFG,Q(E.coli) )
If you want to know other materials,refer to protocol4
procedure
see protocol4
result Although many bands appeared,bands of bcsEFG and bcsQ also appeared
Experiment:Electrophoresis of insert check PCR
member
naoto and bambi75
material
- PCR production (bcsD(A.xylinum) )
If you want to know other materials,refer to protocol4
procedure
see protocol4
Experiment:Purification bcsA(E.coli)
member
naoto and bambi75
material
- bcsQ,EFG(E.coli) in agarose gel
If you want to know other materials,refer to protocol5
procedure
follow to protocol5
Experiment:digestion of bcsEFG,Q(E.coli)
member
naoto and bambi75
material
- bcsQ,EFG(E.coli)
If you want to know other materials,refer to protocol6
procedure
follow to protocol6
Experiment:Electrophoresis of bcsEFG,Q
member
naoto and bambi75
material
- PCR production (bcsEFG,Q(E.coli) )
If you want to know other materials,refer to protocol4
procedure
see protocol4
result Both bands of bcsEFG and bcsQ disappeared
Experiment:Transformation
member
naoto and bambi75
material
- parts of bcsA,B,C,D(A.xylinum) and bcsA(E.coli)
If you want to know other materials,refer to protocol8
procedure
see protocol8
