Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/28

From 2010.igem.org

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(New page: {{:Team:Tokyo_Metropolitan/Header}} ==2010/09/28 Tuesday (Naoto)== ===Experiment:Electrophoresis of insert check PCR=== '''member''' naoto and bambi75 '''material''' *PCR production (b...)
 
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{{:Team:Tokyo_Metropolitan/Header}}
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{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
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__NOTOC__
==2010/09/28 Tuesday (Naoto)==
==2010/09/28 Tuesday (Naoto)==
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===Experiment:Electrophoresis of insert check PCR===
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===Experiment:Electrophoresis of Colony PCR===
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'''member'''
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:'''Member'''
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naoto and bambi75
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:naoto and bambi75
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'''material'''
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:'''Material'''
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*PCR production (bcsD(''A.xylinum'') )
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:*PCR production (bcsD(''A.xylinum'') )
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If you want to know other materials,refer to protocol4
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:If you want to know other materials,refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
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'''procedure'''
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:'''Procedure'''
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see protocol4
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol4</a></html>
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'''result'''
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:'''Result'''
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Bands didn't appear at all
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:Bands didn't appear at all
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===Experiment:Electrophoresis of bcsEFG,Q===
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===Experiment:Transformation===
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'''member'''
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:'''Member'''
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naoto and bambi75
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:naoto and bambi75
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'''material'''
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*PCR production (bcsEFG,Q(''E.coli'') )
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If you want to know other materials,refer to protocol4
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'''procedure'''
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see protocol4
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'''result'''
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Although many bands appeared,bands of bcsEFG and bcsQ also appeared
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===Experiment:Electrophoresis of insert check PCR===
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'''member'''
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naoto and bambi75
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'''material'''
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*PCR production (bcsD(''A.xylinum'') )
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-
If you want to know other materials,refer to protocol4
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'''procedure'''
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see protocol4
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===Experiment:Purification bcsA(''E.coli'')===
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'''member'''
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naoto and bambi75
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'''material'''
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*bcsQ,EFG(''E.coli'') in agarose gel
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If you want to know other materials,refer to protocol5
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'''procedure'''
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follow to protocol5
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===Experiment:digestion of bcsEFG,Q(''E.coli'')===
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'''member'''
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naoto and bambi75
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'''material'''
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*bcsQ,EFG(''E.coli'')
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If you want to know other materials,refer to protocol6
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'''procedure'''
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follow to protocol6
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===Experiment:Electrophoresis of bcsEFG,Q===
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'''member'''
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naoto and bambi75
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+
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'''material'''
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-
 
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*PCR production (bcsEFG,Q(''E.coli'') )
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If you want to know other materials,refer to protocol4
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'''procedure'''
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see protocol4
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'''result'''
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Both bands of bcsEFG and bcsQ disappeared
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===Experiment:Transformation===
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'''member'''
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naoto and bambi75
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:'''Material'''
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'''material'''
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:*parts of bcsA,B,C,D(''A.xylinum'') and bcsA(''E.coli'')
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:If you want to know other materials,refer to <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol9</a></html>
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*parts of bcsA,B,C,D(''A.xylinum'') and bcsA(''E.coli'')
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:'''Procedure'''
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If you want to know other materials,refer to protocol8
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'''procedure'''
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:see <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Electrophoresis">protocol9</a></html>
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see protocol8
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<br/>

Latest revision as of 15:28, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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31

2010/09/28 Tuesday (Naoto)

Experiment:Electrophoresis of Colony PCR

Member
naoto and bambi75
Material
  • PCR production (bcsD(A.xylinum) )
If you want to know other materials,refer to protocol4
Procedure
see protocol4
Result
Bands didn't appear at all

Experiment:Transformation

Member
naoto and bambi75
Material
  • parts of bcsA,B,C,D(A.xylinum) and bcsA(E.coli)
If you want to know other materials,refer to protocol9
Procedure
see protocol9

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