Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/23
From 2010.igem.org
E.coli Fiber Project Notebook
|
|
|
2010/8/23 Monday (watachin)
Experiment:PCR
- Member
- Bambi75 and watachin
- Materials
- E.coli K12 colony
- 10µM/l forward Primer 5µl(bcsA,bcsC)
- 10µM/l reverse Primer 5µl(bcsA,bcsC)
- 10×EX taq buffer 10µl
- 2.5mM each dNTP mixture 10µl
- EX taq polymerase 1µl
- milli-Q 71µl
- Procedure
- see protocol3
Experiment:Restriction enzyme digestion
- Member
- Bambi75 and watachin
- Material
- bcsA
- bcsC
- You can see other material at protocol7
- Procedure
- see protocol7