Project I

Project II

Project II -- Y-hunter

Most of materials we used is from a kit given by a company, Dualsystems Biotech in Swiss Confederation. The kit contains pDHB1 Bait vector, pPR3-N Prey/Library vector, pDHB1-largeT Control bait, pDSL Control prey, pOst1-Nubl Functional control, NMY51 Yeast reporter strain, DUALhunter library, DSY Trafo kit, HTX galactosidase. In addition, we amplify N-terminal of luciferase and C-terminal of luciferase from a part constructed by one IGEM team through PCR. We also obtain a kind of receptor named maltose-binding protein from genome of E.coli. All the equipments we used during our experiment are provided by Tianjin University.

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8.. Remy I, Michnick SW: Application of protein-fragment complementa

1. Brainstorm and make the plan. 2010.5-2010.7

2. Computational design and Mathematical simulation 2010.7-2010.7.25

3. Homology modeling and molecular docking 2010.7.21-2010.8.10

4. Wet experiment. 2010.8.11-2010.10.20

5. Construct biobricks and make summary. 2010.10.20-2010.11