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<div class="leftbartitle">Project</div><br />
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<a href="https://2010.igem.org/Team:Tianjin/notebook1"><h1 class="leftbarheadline">Project I</h1></a><br />
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<a href="https://2010.igem.org/Team:Tianjin/notebook2"><h1 class="leftbarheadline">Project II</h1></a><br />
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<h1 class="righttitle">Project II -- <span class="fontred">Y</span>-hunter</h1><br />
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<div class="rightsubtitle">Material</div><br />
<p class="righttext">Most of materials we used is from a kit given by a company, Dualsystems Biotech in Swiss Confederation. The kit contains pDHB1 Bait vector, pPR3-N Prey/Library vector, pDHB1-largeT Control bait, pDSL Control prey, pOst1-Nubl Functional control, NMY51 Yeast reporter strain, DUALhunter library, DSY Trafo kit, HTX galactosidase. In addition, we amplify N-terminal of luciferase and C-terminal of luciferase from a part constructed by one IGEM team through PCR. We also obtain a kind of receptor named maltose-binding protein from genome of E.coli. All the equipments we used during our experiment are provided by Tianjin University. </p><br />
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<div class="rightsubtitle">Reference</div><br />
<p class="righttext">1. Bishop, A. et al.Unnatural ligands for engineered proteins: new tools for chemical genetics. Annu. Rev.Biophys. Biomol. Struct. 29, 577–606 (2000).<br /><br />
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2. Harris, J. L. & Craik, C. S. Engineering enzyme specificity. Curr.Opin.Chem. Biol. 2, 127–132 (1998).<br /><br />
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3. Bolon, D. N. & Mayo, S. L. Enzyme-like proteins by computational design. Proc. Natl Acad. Sci. USA98, 14274–14279 (2001).<br /><br />
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4. Benson, D. E., Haddy, A. E. & Hellinga, H.W. Converting a maltose receptor into a nascent binuclear oxygenase by computational design. Biochemistry 41, 3262–3267 (2002).<br /><br />
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5. Hellinga,H.W. & Marvin, J. S. Protein engineering and the development of generic biosensors. <br /><br />
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6. Zhang J, Campbell RE, Ting AY, Tsien RY: Creating new fluorescent probes for cell biology. Nat Rev Mol Cell Biol 2002,3:906-918.<br /><br />
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7. Michnick SW, Ear PH, Manderson EN, Remy I, Stefan E: Universal strategies in research and drug discovery based on proteinfragment<br />
complementation assays. Nat Rev Drug Discov 2007,6:569-582.<br /><br />
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8.. Remy I, Michnick SW: Application of protein-fragment complementa</p><br />
<div class="rightsubtitle">Schedule</div><br />
<p class="righttext">1. Brainstorm and make the plan. 2010.5-2010.7<br /><br />
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2. Computational design and Mathematical simulation 2010.7-2010.7.25<br /><br />
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3. Homology modeling and molecular docking 2010.7.21-2010.8.10<br /><br />
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4. Wet experiment. 2010.8.11-2010.10.20<br /><br />
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5. Construct biobricks and make summary. 2010.10.20-2010.11<br />
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Miguyue