Team:The Citadel-Charleston/Project

From 2010.igem.org

(Difference between revisions)
(Prototype team page)
(Overall project)
 
Line 47: Line 47:
== '''Overall project''' ==
== '''Overall project''' ==
-
Your abstract
+
The 2010 Citadel-Charleston Team is interested in the production of satiety peptides by intestinal prokaryotes.  Our project is to engineer an ''E.coli'' chassis to express PYY(3-36), a peptide naturally produced by eukaryotic ileum and colon cells and responsible for the regulation of hunger.  Synthesis of this peptide by local microflora would provide a potentially valuable tool for controlling appetite.
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
 +
We are implementing an AHL-AiiA quorum sensing system coupled with the expression of a poison peptide to strictly regulate colony population size and establish an upper limit to the total amount of PYY generated.  We are also exploring mechanisms for the secretion of the protein into the extracellular space, for the prevention of mutation and genetic transfer, and for the time-based release of our protein.  Our goal is to provide a proof-of-principle for the basic components of a chassis designed to regulate appetite from within the gut and to demonstrate that a safe and effective ''in vivo'' delivery system for neurologically relevant peptides is a compelling direction for research and development.
== Project Details==
== Project Details==

Latest revision as of 19:12, 14 July 2010


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.


You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.

Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)

Team Example


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety



Contents

Overall project

The 2010 Citadel-Charleston Team is interested in the production of satiety peptides by intestinal prokaryotes. Our project is to engineer an E.coli chassis to express PYY(3-36), a peptide naturally produced by eukaryotic ileum and colon cells and responsible for the regulation of hunger. Synthesis of this peptide by local microflora would provide a potentially valuable tool for controlling appetite.

We are implementing an AHL-AiiA quorum sensing system coupled with the expression of a poison peptide to strictly regulate colony population size and establish an upper limit to the total amount of PYY generated. We are also exploring mechanisms for the secretion of the protein into the extracellular space, for the prevention of mutation and genetic transfer, and for the time-based release of our protein. Our goal is to provide a proof-of-principle for the basic components of a chassis designed to regulate appetite from within the gut and to demonstrate that a safe and effective in vivo delivery system for neurologically relevant peptides is a compelling direction for research and development.

Project Details

Part 2

The Experiments

Part 3

Results