Team:TU Delft/protocols/provided biobricks


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Use of Provided Biobricks

From the iGEM organization each team has received a distribution kit containing selected biobricks from the previous years. These biobricks are provided in three 384 well plates containing dried DNA with which competent cells can transformed. The biobricks are supplied as the DNA in a plasmid with a certain antibiotic resistance, which is denoted by a letter in the plasmid name . All the possible antibiotic resistances that are used within the Biobrick standard are ampicillin (A), chloramphenicol (C), erythromycin (E), gentamycin (G), kanamycin (K), neomycin (N), nalidixic acid (Na), rifampicin (R), spectinomycin (S), streptomycin (St), tetracycline (T), trimethoprim (Tm) and zeocin (Z).

Before use, the dried DNA must be resuspended :

1. Punch a hole with a pipette tip through the foil cover into the corresponding well of the desired biobrick part

2. Add 10 μL of diH2O (deionized water)

3. Pipette up and down several times, let sit for a few minutes