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Team:TU Delft/protocols/provided biobricks
From 2010.igem.org
(New page: == Use of Provided Biobricks== From the iGEM organization each team has received a distribution kit containing selected biobricks from the previous years. These biobricks are provided in ...) |
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From the iGEM organization each team has received a distribution kit containing selected biobricks from the previous years. These biobricks are provided in three 384 well plates containing dried DNA with which competent cells can transformed. | From the iGEM organization each team has received a distribution kit containing selected biobricks from the previous years. These biobricks are provided in three 384 well plates containing dried DNA with which competent cells can transformed. | ||
The biobricks are supplied as the DNA in a plasmid with a certain antibiotic resistance, which is denoted by a letter in the plasmid name . All the possible antibiotic resistances that are used within the Biobrick standard are ampicillin (A), chloramphenicol (C), erythromycin (E), gentamycin (G), kanamycin (K), neomycin (N), nalidixic acid (Na), rifampicin (R), spectinomycin (S), streptomycin (St), tetracycline (T), trimethoprim (Tm) and zeocin (Z). | The biobricks are supplied as the DNA in a plasmid with a certain antibiotic resistance, which is denoted by a letter in the plasmid name . All the possible antibiotic resistances that are used within the Biobrick standard are ampicillin (A), chloramphenicol (C), erythromycin (E), gentamycin (G), kanamycin (K), neomycin (N), nalidixic acid (Na), rifampicin (R), spectinomycin (S), streptomycin (St), tetracycline (T), trimethoprim (Tm) and zeocin (Z). | ||
| - | Before use, the dried DNA must be resuspended : | + | Before use, the dried DNA must be resuspended. This protocol is based on Part registry |
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| + | ''Materials:'' | ||
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| + | - BioBrick distribution plates | ||
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| + | - milliQ | ||
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| + | ''Protocol:'' | ||
1. Punch a hole with a pipette tip through the foil cover into the corresponding well of the desired biobrick part | 1. Punch a hole with a pipette tip through the foil cover into the corresponding well of the desired biobrick part | ||
| - | 2. Add 10 μL of | + | 2. Add 10 μL of milliQ |
3. Pipette up and down several times, let sit for a few minutes | 3. Pipette up and down several times, let sit for a few minutes | ||
Latest revision as of 09:19, 12 September 2010
Use of Provided Biobricks
From the iGEM organization each team has received a distribution kit containing selected biobricks from the previous years. These biobricks are provided in three 384 well plates containing dried DNA with which competent cells can transformed. The biobricks are supplied as the DNA in a plasmid with a certain antibiotic resistance, which is denoted by a letter in the plasmid name . All the possible antibiotic resistances that are used within the Biobrick standard are ampicillin (A), chloramphenicol (C), erythromycin (E), gentamycin (G), kanamycin (K), neomycin (N), nalidixic acid (Na), rifampicin (R), spectinomycin (S), streptomycin (St), tetracycline (T), trimethoprim (Tm) and zeocin (Z).
Before use, the dried DNA must be resuspended. This protocol is based on Part registry
Materials:
- BioBrick distribution plates
- milliQ
Protocol:
1. Punch a hole with a pipette tip through the foil cover into the corresponding well of the desired biobrick part
2. Add 10 μL of milliQ
3. Pipette up and down several times, let sit for a few minutes
