Team:TU Delft/protocols/ligation


Revision as of 19:18, 15 July 2010 by Ebrinkman (Talk | contribs)


Ligations (pasting plasmid DNA) were performed at the appropriate temperature with the appropriate buffer in the appropriate concentration, according to the supplier.

Reaction for one sample:

DNA insert x μL
DNA vector x μL
T4 Ligation buffer (10×) 2.0 μL (for 1×)
T4 Ligase 1.0 μL
H2O x μL
20.0 μL (final contration ~20 ng/μL)

Transform circa half of the ligation mix. Incubate at 16 °C o/n