Team:TU Delft/protocols/ligation


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Ligations (pasting plasmid DNA) were performed at the appropriate temperature with the appropriate buffer in the appropriate concentration, according to the supplier.

Reaction for one sample:

DNA insert x μL
DNA vector x μL (for 1×)
T4 Ligation buffer (10×) 2.5 μL (for 1×)
T4 Ligase x μL
H2O x μL
25,0 μL (final contration ~100 ng/μL)

Transform circa half of the ligation mix. Incubate at 16 °C o/n