"
Team:TU Delft/protocols/ligation
From 2010.igem.org
(Difference between revisions)
Ebrinkman (Talk | contribs)
(New page: ==Ligation== Ligations (pasting plasmid DNA) were performed at the appropriate temperature with the appropriate buffer in the appropriate concentration, according to the supplier. React...)
Newer edit →
(New page: ==Ligation== Ligations (pasting plasmid DNA) were performed at the appropriate temperature with the appropriate buffer in the appropriate concentration, according to the supplier. React...)
Newer edit →
Revision as of 13:17, 5 July 2010
Ligation
Ligations (pasting plasmid DNA) were performed at the appropriate temperature with the appropriate buffer in the appropriate concentration, according to the supplier.
Reaction for one sample:
| DNA insert | x μL |
| DNA vector | x μL (for 1×) |
| T4 Ligation buffer (10×) | 2.5 μL (for 1×) |
| T4 Ligase | x μL |
| H2O | x μL |
| 25,0 μL (final contration ~100 ng/μL) |
Transform circa half of the ligation mix. Incubate at 16 °C o/n
