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Team:TU Delft/protocols/freezing bacterial stocks
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(New page: ==Freezing of bacterial stocks== ''Materials:'' - Bacterial culture - LB medium - 80% glycerol - Centrifuge ''Protocol:'' 1. Take 5 mL bacterial cells from the Erlenmeyer of a fre...)
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(New page: ==Freezing of bacterial stocks== ''Materials:'' - Bacterial culture - LB medium - 80% glycerol - Centrifuge ''Protocol:'' 1. Take 5 mL bacterial cells from the Erlenmeyer of a fre...)
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Revision as of 13:07, 5 July 2010
Freezing of bacterial stocks
Materials:
- Bacterial culture
- LB medium
- 80% glycerol
- Centrifuge
Protocol:
1. Take 5 mL bacterial cells from the Erlenmeyer of a freshly grown culture and spin in a 15 mL tube for 10 minutes at 2.000 rpm (Eppendorf centrifuge).
2. Decant the supernatant without disturbing the pellet.
3. Pipet on the pellet 0.5 mL 1% LB medium and 0.5 mL 80% glycerol and mix by vortexing and save in -80 °C freezer.
