Team:TU Delft/protocols/buffers stock solutions

From 2010.igem.org

Buffers and Stock solutions

Luria-bertani (LB)

For 1 liter dissolve in H2O:

Compound/Ingredient Amount required (1L of medium)
Tryptone 10 g
Yeast extract 5 g
NaCl 10 g
Agar+ 15 g

* Add agar in case a solid medium is required.

Autoclave on a liquid cycle. Once broth reaches room temperature, store in cold room.


M9

For 1 liter dissolve in H2O:

Compound/Ingredient Amount required (1L of medium)
Na2HPO4 6 g
KH2PO4 3 g
NaCl 0.5 g
(NH4)Cl 1 g
Agar* 15 g

* Add agar in case a solid medium is required.

Once broth reaches room temperature, add to the broth in aseptic conditions the following amount of each solution:

Solution Amount(1L of medium)
1 M MgSO4 2 mL
1 M CaCl2 0.1 mL
20% Glucose (w/v)* 10 mL
1M Thiamine-HCl 1 mL

* For M9-modified medium no glucose solution is added, instead 10 mL of filtered hydrocarbon/ hydrocarbon mixture are added per liter of medium. Be careful when adding the hydrocarbon/hydrocarbon mixture.

NOTE: Autoclave the solutions 1 M MgSO4, 1M CaCl2, 20% glucose (w/v) (EXCEPT thiamine-HCl 1M) on a liquid cycle. Filter the solution Thiamine-HCl 1M using a 0.22 μm membrane, store the solution in a cold room when is not required.

Store in cold room if the medium is not used.


Antibiotics

Antibiotic Final concentration in the medium
Ampicillin 100 μg/mL
Kanamycin 50 μg/mL
Chlorampenicol 35 μg/mL
Tetracycline 15 μg/mL

All stock solutions must be kept at -20 ºC and used under aseptic conditions.


10x TBE Buffer

For 1 liter, dissolve in 800 mL H2O:

Compound/Ingredient Amount required (1L of medium)
Tris 54 g
Boric Acid 27.5 g
0.5 M EDTA 4.65 g or 20 mL

Adjust volume to 1 liter with H2O