Team:TU Delft/protocols/agarose gel

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(Difference between revisions)
(New page: ==Agarose gel== 1. Dissolve 1 g of agarose in 100 mL 1x TBE buffer for 1% gel (can be stored in the 70 °C stove) 2. Add 5 µL of SYBRSafe (Invitrogen))
 
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==Agarose gel==
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=Agarose gel=
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''Material:''
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- agarose
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- TBE buffer
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 +
- SYBRSafe (Invitrogen)
 +
 
 +
 
 +
''Protocol:''
1. Dissolve 1 g of agarose in 100 mL 1x TBE buffer for 1% gel (can be stored in the 70 °C stove)
1. Dissolve 1 g of agarose in 100 mL 1x TBE buffer for 1% gel (can be stored in the 70 °C stove)
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2. Add 5 µL of SYBRSafe (Invitrogen)
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2. Add 5 µL of SYBRSafe

Latest revision as of 11:09, 12 September 2010

Agarose gel

Material:

- agarose

- TBE buffer

- SYBRSafe (Invitrogen)


Protocol:

1. Dissolve 1 g of agarose in 100 mL 1x TBE buffer for 1% gel (can be stored in the 70 °C stove)

2. Add 5 µL of SYBRSafe