Team:TU Delft/project/rbs characterization
From 2010.igem.org
RBS Characterization
For our RBS characterization project, 5 different RBS sequences from the Anderson RBS family where placed in front of GFP and measured over 18 hours using a Gen5 fluorenscence and absorbance plate reader
Strength was calculated by taking the mean of the ratio between the expression of known RBS (B0032) and expression of Anderson RBS over some time. Expression being the measured fluorescence divided by measured biomass (absorbance, OD).
The measurements where taken from the part of the curve where optimal growth can be assumed, so from 0:40 until 2:30 The result is a simple characterization of the Anderson RBS sequences in relation to the known RBSes.
RBS | Strength |
J61100 | 0.047513 |
J61101 | 0.119831 |
J61107 | 0.065454 |
J61117 | 0.038518 |
J61127 | 0.087334 |
B0032 | 0.300000 |
We've also looked at mRNA folded shapes using mfold to see if there was a common pattern in the Anderson RBS shapes. Unfortunately, this doesn't seem to be the case, as all the tried RBS sequences had very different mfold shapes.