Team:TU Delft/project/rbs characterization
From 2010.igem.org
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(→RBS Characterization) |
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|'''Feature''' | |'''Feature''' | ||
|'''Function''' | |'''Function''' | ||
+ | |- | ||
+ | |[http://partsregistry.org/Part:BBa_B0032 AmpR] | ||
+ | |Ampicillin resistance | ||
|- | |- | ||
|[http://partsregistry.org/Part:BBa_B0015 B0015] | |[http://partsregistry.org/Part:BBa_B0015 B0015] | ||
- | |Transcriptional double terminator | + | |Transcriptional (double) terminator |
|- | |- | ||
|[http://partsregistry.org/Part:BBa_B0062 B0062] | |[http://partsregistry.org/Part:BBa_B0062 B0062] | ||
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|VR primer binding site | |VR primer binding site | ||
|- | |- | ||
- | |[http://partsregistry.org/Part: | + | |[http://partsregistry.org/Part:BBa_J61100 J61100] |
- | | | + | |RBS Anderson family |
+ | |- | ||
+ | |[http://partsregistry.org/Part:BBa_J23100 J23100] | ||
+ | |Promoter | ||
+ | |- | ||
|} | |} | ||
Revision as of 13:23, 10 August 2010
RBS Characterization
For our RBS characterization project, 5 different RBS sequences from the Anderson RBS family (J61100, J61101, J61107, J61117, J61127) and one known RBS (B0032) where placed in front of GFP and measured over 18 hours using a Gen5 fluorenscence and absorbance plate reader. The general map of the construction is shown below, where the RBS is displayed in fucsia.
Feature | Function |
AmpR | Ampicillin resistance |
B0015 | Transcriptional (double) terminator |
B0062 | Transcriptional terminator |
E0040 | GFP |
G00000 | Standard prefix |
G00001 | Standard suffix |
G00100 | VF2 primer binding site |
G00101 | VR primer binding site |
J61100 | RBS Anderson family |
J23100 | Promoter |
RBS | Strength |
J61100 | 0.047513 |
J61101 | 0.119831 |
J61107 | 0.065454 |
J61117 | 0.038518 |
J61127 | 0.087334 |
B0032 | 0.300000 |
We've also looked at mRNA folded shapes using mfold to see if there was a common pattern in the Anderson RBS shapes. This might have been usable in predicting RBS strength for the other untested Anderson RBS sequences. Unfortunately, this didn't seem to be the case, as all the tried RBS sequences had very different mfold shapes.