From 2010.igem.org
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- | =RBS Characterization=
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- | For our RBS characterization project, 5 different RBS sequences from the [http://partsregistry.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Anderson Anderson RBS family] where placed in front of GFP and measured over 18 hours using a Gen5 fluorenscence and absorbance plate reader
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- | [[Image:26_07_2010_Rbs.png]]
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- | Strength was calculated by taking the mean of the ratio between the expression of known RBS ([http://partsregistry.org/Part:BBa_B0032 B0032]) and expression of Anderson RBS over some time. Expression being the measured fluorescence divided by measured biomass (absorbance, OD).
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- | The measurements where taken from the part of the curve where optimal growth can be assumed, so from 0:40 until 2:30
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- | The result is a simple characterization of the Anderson RBS sequences in relation to the known RBSes.
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- | {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
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- | |'''RBS'''
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- | |'''Strength'''
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- | |-
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- | |[http://partsregistry.org/Part:BBa_J61100 J61100]
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- | |0.047513
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- | |[http://partsregistry.org/Part:BBa_J61101 J61101]
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- | |0.119831
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- | |[http://partsregistry.org/Part:BBa_J61107 J61107]
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- | |0.065454
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- | |[http://partsregistry.org/Part:BBa_J61117 J61117]
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- | |0.038518
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- | |-
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- | |[http://partsregistry.org/Part:BBa_J61127 J61127]
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- | |0.087334
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- | |[http://partsregistry.org/Part:BBa_B0032 B0032]
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- | |0.300000
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- | |}
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- | We've also looked at mRNA folded shapes using mfold to see if there was a common pattern in the Anderson RBS shapes. Unfortunately, this doesn't seem to be the case, as all the tried RBS sequences had very different mfold shapes.
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Latest revision as of 13:58, 14 October 2010