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Team:TU Delft/Tour3
From 2010.igem.org
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<h3>Higher slopes equals happy cells</h3> | <h3>Higher slopes equals happy cells</h3> | ||
| - | <p>The tolerance bricks were characterized by measuring growth curves. Once transformed, the cells survived up to 1 molar of salt and 10% of | + | <p>The tolerance bricks were characterized by measuring growth curves. Once transformed, the cells survived up to 1 molar of salt and 10% of n-hexane.</p><p>Clearly, our cells started to enjoy these conditions.</p> |
<p><a href="https://2010.igem.org/Team:TU_Delft/Project/survival/results">Read more</a></p> | <p><a href="https://2010.igem.org/Team:TU_Delft/Project/survival/results">Read more</a></p> | ||
</div> | </div> | ||
Revision as of 09:22, 27 October 2010
Characterization
After cloning comes characterization. We just love it when a plan comes together!
Octane, octanol, octanal...
Lower peaks on the GC graphs made us very proud. The biobricks reduce alkanes up to C30 to thin air.
Enzyme assays confirm that it is not just evaporation that we are looking at.
Higher slopes equals happy cells
The tolerance bricks were characterized by measuring growth curves. Once transformed, the cells survived up to 1 molar of salt and 10% of n-hexane.
Clearly, our cells started to enjoy these conditions.
Shiny GFP on glucose depletion
The sensing parts were connected to a GFP reporter. Our cells could detect the absence of glucose.
New! Emulsification Assay
We created a new assay for the measurement of emulsification assays. Thanks to the hydrophobic dye Sudan II we could determine the strength of the emulsifier.
