Team:TU Delft/Project/tolerance/results


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Salt & Alkane Tolerance Resuls & Conclusions

Salt tolerance

We tested the growth of our bbc1 construct (biobrick # K398108) as stated in our characterization page.

The growth rates were determined for the exponential fase (using a trendline). The results are visible in the following chart.

TU Delft Salt tolerance.jpg

At 0.2 M some effects of the hightened salt stress start to appear, at this concentration our biobrick (BBa_108) shows a significant increase in growth rate in comparison to our background (BBa_027). At higher salt concentrations the salt stress cuases a multitude of effects and as our biobrick simply counters one, our strain also shows effects of higher salt stress. In conclusion one can see that our biobrick is indeed functional at concentrations greater then 0.3 M Sodium Chloride. The general increase in tolerance varies between 10 and 35% depending on the Sodium Chloride concentration.

Alkane tolerance

Future prospects

We hope that the salt tolerance can be increased further by adding systems (such as ion pumps) to ensure the intracellular threshold and minimize the effects of salt stress even further. As the host organism would then be able to maintain growth at lethal concentrations of both salt and alkanes. This would allow future generations of igemmers to create cultures in a variety of different media, opening a new field of possibilities.