Team:TU Delft/Project/tolerance/parts


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== The Biobricks ==

Genes for solvent and salt tolerance were found during our literature review: BBC1, prefoldin alpha and beta. The original sequences of these genes differ in composition from the E. coli sequences; furthermore they contain undesired restriction sites. We decided to enhance our sequences for a successful expression on E. coli, for that purpose we used jcat website tool.

Once we had the enhanced sequence, we added the standard Biobric prefix and suffix. Finally, we sticked the promoter-RBS sequences that best suit for our CDS.

Solvent tolerance For solvent tolerance the following construct was made: J23100- phPFDα J23100 phPFDβ J23100- phPFDα – J23100 phPFDβ

For salt tolerance the following constructs were made: J23100- bbc1-B0015 (Low expression) J23101-bbc1-B0015 (Low to moderate expression J23119-B0034-bbc1-B0015 (High expression)