Team:TU Delft/Project/solubility/results

From 2010.igem.org

(Difference between revisions)
Line 6: Line 6:
===Results===
===Results===
 +
For the production of large amounts of protein, 1 L shake flasks cultures were induced with IPTG after the OD measuremnts started to show the start of the exponential phase. The experiment was done 3 seperate times.
 +
 +
====Experiment 1====
 +
This was the first in a large volume and carried out at 37 C. The OD measurements at the induction time point and harvest are shown in Table 1.
 +
 +
{|
 +
|'''#'''
 +
|'''Culture'''
 +
|'''OD600 at induction'''
 +
|'''OD600 at harvest'''
 +
|-
 +
|1
 +
|Control (J13002) 37 C
 +
|0.367
 +
|0.882
 +
|-
 +
|2
 +
|AlnA (K398206) 37 C
 +
|0.434
 +
|0.880
 +
|}
-
For the production of large amounts of protein, 1 L shake flasks cultures were induced with IPTG after the OD600 was starting to increase towards 0.1 (Table 1).
 
{|
{|

Revision as of 10:27, 20 October 2010

Hydrocarbon Solubility Results & Conclusions

Our goal was to enhance the solubility of alkanes in water. Therefore we constructed a new part that expresses the emulsifier protein AlnA after induction by IPTG. By using our own emulsification assay we measured the increase in hydrophobic compounds in the water phase.

Results

For the production of large amounts of protein, 1 L shake flasks cultures were induced with IPTG after the OD measuremnts started to show the start of the exponential phase. The experiment was done 3 seperate times.

Experiment 1

This was the first in a large volume and carried out at 37 C. The OD measurements at the induction time point and harvest are shown in Table 1.

# Culture OD600 at induction OD600 at harvest
1 Control (J13002) 37 C 0.367 0.882
2 AlnA (K398206) 37 C 0.434 0.880


# Culture OD600 at induction OD600 at harvest
1 Control (J13002) 30 C 0.073 0.451
2 Control (J13002) 37 C 0.088 0.810
3 AlnA (K398206) 30 C 0.060 0.393
4 AlnA (K398206) 37 C 0.067 0.624

Conclusions

jadie jadie jadie