Team:TU Delft/Project/sensing/characterization

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(Difference between revisions)
(Characterization of the Sensing Parts)
(Characterized Strains)
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Strains:  
Strains:  
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* PalkS / AlkS: E.coli K12/310C
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* P(CaiF)-E0240: E.coli K12/331A
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* PalkS / AlkS / PalkB: E.coli K12/311C
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* Negative control: ''E.coli K12'' carrying J13002 on the empty plasmid pSB1A2.
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* P(CaiF) / AlkS / PalkB: E.coli K12/312C
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* Negative control: ''E.coli K12''
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The characterization process of the hydrocarbon sensing sub-project will entail output measurements of fluorescence at varying octane concentrations (0.1% – 1%) of the above mentioned strains. These measurements will be performed using a 96-well plate reader. Growth curves will be followed by OD-600 alongside the fluorescence measurements. The obtained fluorescence and growth measurements will be analyzed and modeled accordingly.
 
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Revision as of 13:21, 25 October 2010

CharacterizationResultsParts

Characterization of the Sensing Parts

Characterized Strains

Strains:

  • P(CaiF)-E0240: E.coli K12/331A
  • Negative control: E.coli K12 carrying J13002 on the empty plasmid pSB1A2.


CharacterizationResultsParts