Team:TU Delft/Project/rbs-characterization/results

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(The Results)
(The Results)
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==The Results==
==The Results==
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In the boxplot below the RBS strengths for the five tested members of the Anderson family are given along with the deviations from the mean. The strengths are given relative to the community RBS BBa_B0034, which has a strength of unity (1). This relationship could be built by our characterizing the community RBS [http://partsregistry.org/Part:BBa_B0032 BBa_B0032] simultaneously, which is known to have a strength of 0.3 relative to [http://partsregistry.org/Part:BBa_B0032 BBa_B0034]. Therefore in the plot below, the mean value of BBa_B0032 is fixed at 0.3.
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In the boxplot below the RBS strengths for the five tested members of the Anderson family are given along with the deviations from the mean. The strengths are given relative to the community RBS BBa_B0034, which has a strength of unity (1). This relationship could be built by our characterizing the community RBS [http://partsregistry.org/Part:BBa_B0032 BBa_B0032] simultaneously, which is known to have a strength (or efficiency) of 0.3 relative to [http://partsregistry.org/Part:BBa_B0032 BBa_B0034]. Therefore in the plot below, the mean value of BBa_B0032 is fixed at 0.3.
[[Image:TUDelft2010_Rbs_strength_boxplot.png]]
[[Image:TUDelft2010_Rbs_strength_boxplot.png]]
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<html><div id="rbs-results"></html>
<html><div id="rbs-results"></html>
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Compared to the B0032, the tested Anderson RBS'es turned out to be surprisingly weak. All 5 of them resulted in less expression than the reference expression.
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==Conclusions==
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We've succeeded in characterizing five memebers of the Anderson ribosome binding site family by simple culturing on a 96 well plate while measuring GFP fluorescence and biomass absorbance. Fitting the obtained data to our improved [http://2010.igem.org/Team:TU_Delft#page=Project/rbs-characterization/characterization protein production model] yielded RBS strengths values with negligible standard deviations.
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Revision as of 10:03, 24 October 2010

CharacterizationResultsParts

The Results

In the boxplot below the RBS strengths for the five tested members of the Anderson family are given along with the deviations from the mean. The strengths are given relative to the community RBS BBa_B0034, which has a strength of unity (1). This relationship could be built by our characterizing the community RBS BBa_B0032 simultaneously, which is known to have a strength (or efficiency) of 0.3 relative to BBa_B0034. Therefore in the plot below, the mean value of BBa_B0032 is fixed at 0.3.

TUDelft2010 Rbs strength boxplot.png

Conclusions

We've succeeded in characterizing five memebers of the Anderson ribosome binding site family by simple culturing on a 96 well plate while measuring GFP fluorescence and biomass absorbance. Fitting the obtained data to our improved protein production model yielded RBS strengths values with negligible standard deviations.

RBS Mean strength Standard deviation
J61100 0.026560 0.005126
J61117 0.123819 0.024725
J61101 0.081210 0.013402
J61107 0.019763 0.003963
J61127 0.069657 0.007422
B0032 0.300000 0.028316

CharacterizationResultsParts