Team:TU Delft/Project/rbs-characterization/results

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<html><center><img src="https://static.igem.org/mediawiki/2010/0/00/TU_Delft_project_navigation.jpg" usemap="#projectnavigation" border="0" /></center><map id="projectnavigation" name="projectnavigation"><area shape="rect" alt="Characterization" title="" coords="309,3,591,45" href="https://2010.igem.org/Team:TU_Delft#page=Project/rbs-characterization/characterization" target="" /><area shape="rect" alt="Results" title="" coords="609,3,891,44" href="https://2010.igem.org/Team:TU_Delft#page=Project/rbs-characterization/results" target="" /><area shape="rect" alt="Parts" title="" coords="9,3,290,44" href="https://2010.igem.org/Team:TU_Delft#page=Project/rbs-characterization/parts" target="" /></map></html>
 
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==RBS Characterization Results==
 
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==RBS Characterization Results==
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<html><center><img src="https://static.igem.org/mediawiki/2010/0/00/TU_Delft_project_navigation.jpg" usemap="#projectnavigation" border="0" /></center><map id="projectnavigation" name="projectnavigation"><area shape="rect" alt="Characterization" title="" coords="309,3,591,45" href="https://2010.igem.org/Team:TU_Delft#page=Project/rbs-characterization/characterization" target="" /><area shape="rect" alt="Results" title="" coords="609,3,891,44" href="https://2010.igem.org/Team:TU_Delft#page=Project/rbs-characterization/results" target="" /><area shape="rect" alt="Parts" title="" coords="9,3,290,44" href="https://2010.igem.org/Team:TU_Delft#page=Project/rbs-characterization/parts" target="" /></map></html>
The given relative strengths are displayed assuming B0034 as the unit (The standard as used by the partsregistry).  
The given relative strengths are displayed assuming B0034 as the unit (The standard as used by the partsregistry).  
B0032 is defined to have a strength of 0.3. Therefore in the plot below, the mean value of B0032 is fixed on 0.3.
B0032 is defined to have a strength of 0.3. Therefore in the plot below, the mean value of B0032 is fixed on 0.3.

Revision as of 14:13, 22 October 2010

RBS Characterization Results

CharacterizationResultsParts The given relative strengths are displayed assuming B0034 as the unit (The standard as used by the partsregistry). B0032 is defined to have a strength of 0.3. Therefore in the plot below, the mean value of B0032 is fixed on 0.3.


TUDelft2010 Rbs strength boxplot.png

Compared to the B0032, the tested Anderson RBS'es turned out to be surprisingly weak. All 5 of them resulted in less expression than the reference expression.

RBS Mean strength Standard deviation
J61100 0.026560 0.005126
J61117 0.123819 0.024725
J61101 0.081210 0.013402
J61107 0.019763 0.003963
J61127 0.069657 0.007422
B0032 0.300000 0.028316

Continue Reading about the RBS Characterization

CharacterizationResultsParts